Metal raf üretim fabrikasında çalışan işçilerde kan ve idrar oksidatif stres parametreleri

Naci Polat; Ahmet Kılınç; Suha Yalçın

doi:10.5472/MMJ.2012.02454.2

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Yıl 2013, Cilt: 26 Sayı: 1, 25 - 29, 30.09.2015

Naci Polat Ahmet Kılınç Suha Yalçın

https://doi.org/10.5472/MMJ.2012.02454.2

Öz

Objectives: We have investigated the level of antioxidants (glutathione (GSH), GSH-reductase, GSH-peroxidase, GSHtransferase, catalase (CAT) and superoxide dismutase (SOD)), lipid peroxidation products (plasma, erythrocyte and urinary malondialdehyde (MDA)) and urinary DNA damage product 8-hydroxydeoxyguanosine (8-OH-2’-dG) in metal-shelf factory workers and compared the levels to those of a control group working in a different work area.Methods: Twenty four workers working in a metal shelf production factory and sixteen apparently healthy male volunteers without environmental and work place exposure were included in the study. Blood and urine samples were examined for oxidative stress parameters. Measurement of 8-OH-2’-dG was by high performance liquid chromatography (HPLC), while other parameters were measured by colorimetric methods.Results: GSH-transferase, CAT, SOD activities and plasma, erythrocyte and urinary MDA levels of metal shelf production factory workers were higher than for the control group, whereas, GSH-peroxidase and GSH in workers were lower than for controls. Moreover, while GSH-reductase activity was lower, 8-OH-2’-dG level was higher than the control group but the difference was not statistically significant.Conclusion: As a result of increased industrialization, air pollution and hazardous working conditions there is a deterioration of the balance between oxidant and antioxidant levels which affects the structure of macromolecules such as DNA, lipids and proteins

Anahtar Kelimeler

Antioxidants, Metal-shelf factory workers, Lipid peroxidation, Oxidative stress, Free radicals

Kaynakça

1. Cheeseman KH, Slater TF.An introduction to free radical biochemistry. Br Med Bull 1993; 49: 481-93.
2. Halliwell B, Gutteridge JMC, (editors).Free radicals in biology and medicine. 3rd ed. Oxford: Oxford Univ Press, 1999.
3. Knight JA. Disease related to oxygen-derived free radicals. Ann Clin Lab Sci 1995; 25: 111-21.
4. Dizdaroglu M, Jaruga P, Birincioglu M, Rodriguez H. Free radicalinduced damage to DNA: Mechanisms and measurement. Free Rad Biol Med 2002; 32: 1102-15.
5. Gutteridge JMC, Halliwell B. Antioxidants: molecules, medicines, and myths. Biochem Biophys Res Commun 2010; 393: 561–4.
6. Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995; 41: 1819–28.
7. Jomova K, Valko M. Advances in metal-induced oxidative stress and human disease. Toxicology 2011; 283: 65-87. http://dx.doi. org/10.1016/j.tox.2011.03.001
8. Sugiyama M. Role of cellular antioxidants in metal-induced damage. Cell Biol Toxicol 1994; 10: 1-22. doi:10.1007/BF00757183
9. Cross CE, Halliwell B, Borish ET, et al. Oxygen radicals and human disease. Ann Intern Med 1987; 107: 526–45.
10. Yagi K. Lipid peroxides in human diseases. Chem Phys Lipids 1987; 45: 337–51. doi:10.1016/0009-3084(87)90071-5
11. Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 1990; 9: 515–40. doi:10.1016/0891- 5849(90)90131-2
12. Yang W, Omaye ST. Air pollutants, oxidative stress and human health. Mutation Res 2009; 674: 45–54.
13. Gupta A, Shukla GS. Enzymatic antioxidants in erythrocytes following heavy metal exposure: Possible role in early diagnosis of poisoning. Bull Environ Contam Toxicol 1997; 58: 198-205. doi:10.1007/ s001289900320
14. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-90.
15. Beutler E. Effect of flavin compounds on glutathione reductase activity in vivo and in vitro studies. J Clin Invest 1969; 48: 1957-66. doi:10.1172/JCI106162
16. Beutler E, (editor). Glutathione peroxidase, Red Cell Metabolism. A manual of biochemical methods. 2nd ed. New York: Grune and Stratton, 1975; 71-3.
17. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249: 7130-9.
18. Chance B. Catalases and peroxidases, Part II. Special Methods: Methods of Biochemical Analysis 1954; 1: 408-24.
19. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500.
20. Stocks J, Offerman EL, Modell CB, Dormandy TL. The susceptibility to autoxidation of human red cell lipids in health and disease. Br J Hematol 1972; 23: 713-24. doi:10.1111/j.1365-2141.1972.tb03486.x
21. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-8. doi:10.1016/0003-2697(79)90738-3
22. Domijian AM, Peracia M. Electrochemical HPLC measurement of 8-oxo-dG. Arh Hig Rada Toksikol 2008; 59: 277-82.
23. De Martinis BS, de Lourdes Pires Bianchi M. Methodology for urinary 8-hydroxy 2’-deoxyguanosine analysis by HPLC with electrochemical detection. Pharmacol Res 2002; 46: 129-31.
24. Schröder P, Krutmann J. Environmental oxidative stress – Environmental sources of ROS. The Handbook of Environmental Chemistry Vol 2 Part O. Berlin, Heidelberg:Springer-Verlag, 2005; 19- 31.
25. Peluso M, Srivatanakul P, Munnia A, et al. Malondialdehydedeoxyguanosine adducts among workers of a Thai industrial estate and nearby residents. Environ Health Perspect 2010; 118: 55-9.
26. Loft S, Poulsen HE, Vistisen K, Knudsen LE. Increased urinary excretion of 8-oxo-2’-deoxyguanosine, a biomarker of oxidative DNA damage, in urban bus drivers. Mutat Res 1999; 441: 11-9.
27. Özgüner MF, Delibaş N, Tahan V, Koyu A, Köylü H. Effects of industrial noise on the blood levels of superoxide dismutase, glutathione peroxidase and malondialdehyde. East J Med 1999; 4: 13- 5.
28. Gayathri MR, Beena VS, Sudha K. Evaluation of lead toxicity and antioxidants in battery workers. Biomed Res 2007; 19: 1-4.
29. Kamal AA, el Khafif M, Koraah S, Massoud A, Caillard JF. Blood superoxide dismutase and plasma malondialdehyde among workers exposed to asbestos. Am J Ind Med 1992; 21: 353-61. doi:10.1002/ ajim.4700210308
30. Sati PC, Khaliq F, Vaney N, Ahmed T, Tripathi AK, Banerjee BD. Pulmonary function and oxidative stress in workers exposed to styrene in plastic factory: occupational hazards in styrene-exposed plastic factory workers. Hum Exp Toxicol 2011; 30: 1743-50. doi: 10.1177/0960327111401436

Metal raf üretim fabrikasında çalışan işçilerde kan ve idrar oksidatif stres parametreleri

Yıl 2013, Cilt: 26 Sayı: 1, 25 - 29, 30.09.2015

Naci Polat Ahmet Kılınç Suha Yalçın

https://doi.org/10.5472/MMJ.2012.02454.2

Öz

Amaç: Bu çalışmada, fabrika ortamında çalışan işçilerin antioksidan
seviyeleri (glutatyon, glutatyon redüktaz, glutatyon peroksidaz,
glutatyon transferaz, katalaz ve süperoksit dismutaz), lipit
peroksidasyon ürünleri (plazma, eritrosit ve idrarda malondialdehit)
ve DNA hasar ürünü 8-hidroksideoksiguanozin (8-OH-2’-dG)
ölçülerek işçilerdeki oksidatif stres düzeylerinin karşılaştırılması
amaçlanmıştır.
Yöntem: Çalışmaya İstanbul sanayi bölgesinde metal raf
üretimi yapan bir fabrikanın üretim kısmında çalışan 24 işçi ile 16
kişiden oluşan bir kontrol grubu alınmış, hastalardan ve kontrol
gruplarından alınan kan ve idrar örneklerinde oksidatif stres
parametreleri incelenmiştir.
Bulgular: İşçilerde glutatyon transferaz, katalaz, süperoksit
dismutaz ve plazma, idrar ve eritrosit malondialdehit düzeyleri
kontrol grubuna göre yüksek bulunurken, glutatyon peroksidaz
ve glutatyon düzeyleri düşük bulunmuştur. Glutatyon redüktaz
ve 8-OH-2’-dG değerleri kontrol grubuna göre sırasıyla düşük
ve yüksek bulunmuş olmakla birlikte değerler arasındaki fark
istatistiksel olarak anlamlı değildir.
Sonuç: Metal raf üretim fabrikasında çalışan işçiler kontrol
grubuna göre serbest radikallerin zararlı etkilerine daha fazla maruz
kalmaktadır.

Anahtar Kelimeler

Antioksidan, Metal raf fabrika işçileri, Lipit peroksidasyonu, Oksidatif stres, Serbest radikaller

Kaynakça

1. Cheeseman KH, Slater TF.An introduction to free radical biochemistry. Br Med Bull 1993; 49: 481-93.
2. Halliwell B, Gutteridge JMC, (editors).Free radicals in biology and medicine. 3rd ed. Oxford: Oxford Univ Press, 1999.
3. Knight JA. Disease related to oxygen-derived free radicals. Ann Clin Lab Sci 1995; 25: 111-21.
4. Dizdaroglu M, Jaruga P, Birincioglu M, Rodriguez H. Free radicalinduced damage to DNA: Mechanisms and measurement. Free Rad Biol Med 2002; 32: 1102-15.
5. Gutteridge JMC, Halliwell B. Antioxidants: molecules, medicines, and myths. Biochem Biophys Res Commun 2010; 393: 561–4.
6. Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995; 41: 1819–28.
7. Jomova K, Valko M. Advances in metal-induced oxidative stress and human disease. Toxicology 2011; 283: 65-87. http://dx.doi. org/10.1016/j.tox.2011.03.001
8. Sugiyama M. Role of cellular antioxidants in metal-induced damage. Cell Biol Toxicol 1994; 10: 1-22. doi:10.1007/BF00757183
9. Cross CE, Halliwell B, Borish ET, et al. Oxygen radicals and human disease. Ann Intern Med 1987; 107: 526–45.
10. Yagi K. Lipid peroxides in human diseases. Chem Phys Lipids 1987; 45: 337–51. doi:10.1016/0009-3084(87)90071-5
11. Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 1990; 9: 515–40. doi:10.1016/0891- 5849(90)90131-2
12. Yang W, Omaye ST. Air pollutants, oxidative stress and human health. Mutation Res 2009; 674: 45–54.
13. Gupta A, Shukla GS. Enzymatic antioxidants in erythrocytes following heavy metal exposure: Possible role in early diagnosis of poisoning. Bull Environ Contam Toxicol 1997; 58: 198-205. doi:10.1007/ s001289900320
14. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-90.
15. Beutler E. Effect of flavin compounds on glutathione reductase activity in vivo and in vitro studies. J Clin Invest 1969; 48: 1957-66. doi:10.1172/JCI106162
16. Beutler E, (editor). Glutathione peroxidase, Red Cell Metabolism. A manual of biochemical methods. 2nd ed. New York: Grune and Stratton, 1975; 71-3.
17. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249: 7130-9.
18. Chance B. Catalases and peroxidases, Part II. Special Methods: Methods of Biochemical Analysis 1954; 1: 408-24.
19. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500.
20. Stocks J, Offerman EL, Modell CB, Dormandy TL. The susceptibility to autoxidation of human red cell lipids in health and disease. Br J Hematol 1972; 23: 713-24. doi:10.1111/j.1365-2141.1972.tb03486.x
21. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-8. doi:10.1016/0003-2697(79)90738-3
22. Domijian AM, Peracia M. Electrochemical HPLC measurement of 8-oxo-dG. Arh Hig Rada Toksikol 2008; 59: 277-82.
23. De Martinis BS, de Lourdes Pires Bianchi M. Methodology for urinary 8-hydroxy 2’-deoxyguanosine analysis by HPLC with electrochemical detection. Pharmacol Res 2002; 46: 129-31.
24. Schröder P, Krutmann J. Environmental oxidative stress – Environmental sources of ROS. The Handbook of Environmental Chemistry Vol 2 Part O. Berlin, Heidelberg:Springer-Verlag, 2005; 19- 31.
25. Peluso M, Srivatanakul P, Munnia A, et al. Malondialdehydedeoxyguanosine adducts among workers of a Thai industrial estate and nearby residents. Environ Health Perspect 2010; 118: 55-9.
26. Loft S, Poulsen HE, Vistisen K, Knudsen LE. Increased urinary excretion of 8-oxo-2’-deoxyguanosine, a biomarker of oxidative DNA damage, in urban bus drivers. Mutat Res 1999; 441: 11-9.
27. Özgüner MF, Delibaş N, Tahan V, Koyu A, Köylü H. Effects of industrial noise on the blood levels of superoxide dismutase, glutathione peroxidase and malondialdehyde. East J Med 1999; 4: 13- 5.
28. Gayathri MR, Beena VS, Sudha K. Evaluation of lead toxicity and antioxidants in battery workers. Biomed Res 2007; 19: 1-4.
29. Kamal AA, el Khafif M, Koraah S, Massoud A, Caillard JF. Blood superoxide dismutase and plasma malondialdehyde among workers exposed to asbestos. Am J Ind Med 1992; 21: 353-61. doi:10.1002/ ajim.4700210308
30. Sati PC, Khaliq F, Vaney N, Ahmed T, Tripathi AK, Banerjee BD. Pulmonary function and oxidative stress in workers exposed to styrene in plastic factory: occupational hazards in styrene-exposed plastic factory workers. Hum Exp Toxicol 2011; 30: 1743-50. doi: 10.1177/0960327111401436

Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	Türkçe
Bölüm	Makaleler
Yazarlar	Naci Polat Bu kişi benim Ahmet Kılınç Bu kişi benim Suha Yalçın
Yayımlanma Tarihi	30 Eylül 2015
Yayımlandığı Sayı	Yıl 2013 Cilt: 26 Sayı: 1

Kaynak Göster

APA	Polat, N., Kılınç, A., & Yalçın, S. (2015). Metal raf üretim fabrikasında çalışan işçilerde kan ve idrar oksidatif stres parametreleri. Marmara Medical Journal, 26(1), 25-29. https://doi.org/10.5472/MMJ.2012.02454.2
AMA	Polat N, Kılınç A, Yalçın S. Metal raf üretim fabrikasında çalışan işçilerde kan ve idrar oksidatif stres parametreleri. Marmara Med J. Ekim 2015;26(1):25-29. doi:10.5472/MMJ.2012.02454.2
Chicago	Polat, Naci, Ahmet Kılınç, ve Suha Yalçın. “Metal Raf üretim fabrikasında çalışan işçilerde Kan Ve Idrar Oksidatif Stres Parametreleri”. Marmara Medical Journal 26, sy. 1 (Ekim 2015): 25-29. https://doi.org/10.5472/MMJ.2012.02454.2.
EndNote	Polat N, Kılınç A, Yalçın S (01 Ekim 2015) Metal raf üretim fabrikasında çalışan işçilerde kan ve idrar oksidatif stres parametreleri. Marmara Medical Journal 26 1 25–29.
IEEE	N. Polat, A. Kılınç, ve S. Yalçın, “Metal raf üretim fabrikasında çalışan işçilerde kan ve idrar oksidatif stres parametreleri”, Marmara Med J, c. 26, sy. 1, ss. 25–29, 2015, doi: 10.5472/MMJ.2012.02454.2.
ISNAD	Polat, Naci vd. “Metal Raf üretim fabrikasında çalışan işçilerde Kan Ve Idrar Oksidatif Stres Parametreleri”. Marmara Medical Journal 26/1 (Ekim 2015), 25-29. https://doi.org/10.5472/MMJ.2012.02454.2.
JAMA	Polat N, Kılınç A, Yalçın S. Metal raf üretim fabrikasında çalışan işçilerde kan ve idrar oksidatif stres parametreleri. Marmara Med J. 2015;26:25–29.
MLA	Polat, Naci vd. “Metal Raf üretim fabrikasında çalışan işçilerde Kan Ve Idrar Oksidatif Stres Parametreleri”. Marmara Medical Journal, c. 26, sy. 1, 2015, ss. 25-29, doi:10.5472/MMJ.2012.02454.2.
Vancouver	Polat N, Kılınç A, Yalçın S. Metal raf üretim fabrikasında çalışan işçilerde kan ve idrar oksidatif stres parametreleri. Marmara Med J. 2015;26(1):25-9.

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