Yıl 2019, Cilt 32 , Sayı 3, Sayfalar 137 - 143 2019-10-31

The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor

Didem KART [1]


Objectives: Interspecies interactions in poly-species biofilm are substantial. Our aim is to set up dynamic biofilm models of
Enterococcus faecalis and Proteus mirabilis using Drip Flow Biofilm Reactor (DFR) and to evaluate the effect of these dual population
on anti-biofilms of some antimicrobials.
Materials and Methods: E.faecalis and P.mirabilis biofilms were formed in a DFR. Influences of the dual interactions on their
susceptibilities to antimicrobial agents (disinfectants, antibiotics and probiotic strains) were determined.
Results: Gluteraldehyde and quaternary ammonium compounds (QAC) effectively killed the cells in both biofilms of E.faecalis and
P.mirabilis. However, the efficacy of hydrogen peroxide (H2O2) was dependant on the microbial species present. P. mirabilis was less
susceptible to the ampicillin and ciprofloxacin in co-culture compared to when cultured alone. Here, the influence of the presence
of E.faecalis on P.mirabilis susceptibility was determined. For high concentrations of ciprofloxacin (1024 and 512 μg/ml), the log
reduction in P.mirabilis cells was determined as approximately 4.5 and 3.5 in mono and dual-species biofilms respectively. Compared
to B.lactis, L.acidophilus was found to be more effective both on single and dual species.
Conclusion: The effect of antimicrobial agents on microbial cells in a polymicrobial biofilm may depend on the composition of the
biofilm.
Polymicrobial biofilm, E. faecalis, P. mirabilis, Antimicrobial, Drip flow reactor, Probiotic strains
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Birincil Dil en
Konular Tıp
Bölüm Original Research
Yazarlar

Orcid: 0000-0001-7119-5763
Yazar: Didem KART (Sorumlu Yazar)

Tarihler

Yayımlanma Tarihi : 31 Ekim 2019

Bibtex @araştırma makalesi { marumj637153, journal = {Marmara Medical Journal}, issn = {1019-1941}, eissn = {1309-9469}, address = {}, publisher = {Marmara Üniversitesi}, year = {2019}, volume = {32}, pages = {137 - 143}, doi = {10.5472/marumj.637153}, title = {The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor}, key = {cite}, author = {KART, Didem} }
APA KART, D . (2019). The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor. Marmara Medical Journal , 32 (3) , 137-143 . DOI: 10.5472/marumj.637153
MLA KART, D . "The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor". Marmara Medical Journal 32 (2019 ): 137-143 <https://dergipark.org.tr/tr/pub/marumj/issue/49745/637153>
Chicago KART, D . "The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor". Marmara Medical Journal 32 (2019 ): 137-143
RIS TY - JOUR T1 - The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor AU - Didem KART Y1 - 2019 PY - 2019 N1 - doi: 10.5472/marumj.637153 DO - 10.5472/marumj.637153 T2 - Marmara Medical Journal JF - Journal JO - JOR SP - 137 EP - 143 VL - 32 IS - 3 SN - 1019-1941-1309-9469 M3 - doi: 10.5472/marumj.637153 UR - https://doi.org/10.5472/marumj.637153 Y2 - 2019 ER -
EndNote %0 Marmara Medical Journal The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor %A Didem KART %T The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor %D 2019 %J Marmara Medical Journal %P 1019-1941-1309-9469 %V 32 %N 3 %R doi: 10.5472/marumj.637153 %U 10.5472/marumj.637153
ISNAD KART, Didem . "The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor". Marmara Medical Journal 32 / 3 (Ekim 2019): 137-143 . https://doi.org/10.5472/marumj.637153
AMA KART D . The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor. Marmara Med J. 2019; 32(3): 137-143.
Vancouver KART D . The susceptibility of Proteus mirabilis and Enterococcus faecalis to various antimicrobial agents in polymicrobial biofilms formed using a drip flow reactor. Marmara Medical Journal. 2019; 32(3): 143-137.