Resveratrol supplementation protects against chronic nicotine-induced oxidative damage and organ dysfunction in the rat urogenital system

Abstract

ABSTRACT: The protective effect of resveratrol against nicotine induced oxidative damage
on urogenital tissues was evaluated by biochemical, histological and functional studies.
Wistar Albino rats were injected with either nicotine hydrogen bitartarate (0.6 mg/kg/day, ip)
or saline. Resveratrol (10 mg/kg, po) was administered along with saline or nicotine injections
for 28 days. After decapitation, the urinary bladder, corpus cavernosum and kidney
tissues were excised. Corpus cavernosum and bladder tissues were used for in vitro contractility
studies, or stored at -80 ºC along with kidney tissue for the measurement of malondialdehyde
(MDA), glutathione (GSH), and luminol-lucigenin chemiluminescence (CL) levels.
Tissue samples were also examined histologically. Chronic nicotine administration caused a
significant decrease in GSH levels and increases in MDA levels, and luminol-lucigenin CL in
kidney, urinary bladder and corpus cavernosum tissues, suggesting oxidative organ damage,
which was also verified histologically. In serum samples increased blood urea nitrogen
(BUN), creatinine, proinflammatory cytokines (TNF-

Keywords

• resveratrol, nicotine, kidney, urinary bladder, corpus cavernosum, oxidative damage

Resveratrol supplementation protects against chronic nicotine-induced oxidative damage and organ dysfunction in the rat urogenital system

Abstract

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