Antimicrobial and cytotoxic activities of Tripleurospermum parviflorum (Willd.) Pobed

Abstract

ABSTRACT: The antimicrobial and cytotoxic activities of n-hexane, methanol, ethanol, ethyl
acetate and water extracts of Tripleurospermum parviflorum (Willd.) Pobed. were evaluated
in this study. The antimicrobial activities of the extracts were reported against Escherichia
coli ATCC 29998, Escherichia coli ATCC 25922, Escherichia coli ATCC 11230, Staphylococcus
aureus ATCC 6538P, Staphylococcus aureus ATCC 29213, Enterobacter cloacae ATCC
13047, Enterococcus faecalis ATCC 29212, Pseudomonas aeroginosa ATCC 27853 as bacteria
and Candida albicans ATCC 10239 as yeastlike fungi by disc diffusion method. Cytotoxic
activity of the extracts was tested in vitro against Brine shrimp. All of the extracts showed a
range of activity against the tested bacteria and brine shrimp.
KEY WORDS: Tripleurospermum parviflorum, Asteraceae, cytotoxic activity, antimicrobial activity

Keywords

• Tripleurospermum parviflorum, Asteraceae, cytotoxic activity, antimicrobial activity.

Antimicrobial and cytotoxic activities of

Abstract

Bu çalışmada, Tripleurospermum parviflorum (Willd.) Pobed.’nin n-hegzan, metanol, etanol, etil asetat ve su ekstrelerinin, antimikrobiyal ve sitotoksik aktiviteleri değerlendirildi. Ekstrelerin antimikrobiyal aktiviteleri, bakteri olarak Escherichia coli ATCC 29998, Escherichia coli ATCC 25922, Escherichia coli ATCC 11230, Staphylococcus aureus ATCC6538P, Staphylococcus aureus ATCC 29213, Enterobacter cloacae ATCC 13047, Enterococcus faecalis ATCC 29212, Pseudomonas aeroginosa ATCC 27853 ve mantar olarak Candida albicans ATCC 10239’a karşı disk difüzyon metodu ile tayin edildi. Ekstrelerin sitotoksik aktiviteleri Brine shrimp yöntemiyle değerlendirildi. Ekstrelerin hepsi denenen bakterilere ve Brine shrimp’e karşı anlamlı aktivite gösterdi.

Keywords

• Tripleurospermum parviflorum, Asteraceae, sitotoksik aktivite, antimikrobiyal aktivite.

References

1. Bremer K, Humphries C. Generic Monograph of the Asteraceae - Anthemideae. Bull Nat Hist Mus Bot 1993; 23: 71-177.
2. Yıldırımlı S. The Chorology of the Turkish Species of Asteraceae Family. The Herb J Systemat Bot 1999; 6: 75-123. Cakilcioglu U, Turkoglu I. An Ethnobotanical Survey of Medicinal Plants in Sivrice (Elazığ-Turkey). J Ethnopharmacol 2010; 132: 165-75.
3. Altundag E, Ozturk M. Ethnomedicinal Studies on the Plant Resources of East Anatolia, Turkey. Procedia - Social and Behavioral Sciences 2011; 19: 756-77.
4. Hosseini M, Parvini S, Bakhtiarian A. Anti-inflammatory, Analgesic Activity of Tripleurospermum disciforme Extracts in Rats. Toxicol Mech Method 2007; 17: 575-80. Hooper D, Field H, Dahlgrey BE. Useful Plants and Drugs of Iran and Iraq. In: Field Museum of Natural History, Chicago. 1937, pp. 84-180.
5. Mc Laughlin JL, Chang CJ, Smith DL. Bench-Top Bioassays the Discovery of Bioactive Natural Products: an update. Nat Prod Chem 1991; 9: 383-97.
6. Yasar A, Ucüncü O, Gülec C, İncer H, Ayaz S, Yayla N. GC-Ms Analysis of Chloroform Extracts in Flowers, Stems and Roots of Tripleurospermum callosum. Pharm Biol 2005; 43: 108-12.
7. Wichtl M. Herbal Drugs and Phytopharmaceuticals. Medpharm, Scientific Publishers, Stuttgart. 2004.