Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population

Abstract

Glucose transporter 1 (GLUT1) is located in basolateral side of cells and mediates the unidirectional transport of glucose as non-insulin dependent glucose transporter protein. It is responsible for transportation of glucose especially to cancer cells and also transports arsenic compounds that is related with diabetes mellitus. The present study aimed to detect the allele and genotype frequencies of GLUT1 2841A>T single nucleotide polymorphism (SNP) in Turkish population. Blood samples were collected from unrelated 100 male and 110 female. Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) technique was used to analyze the blood samples. The distribution of 2841A>T SNP was 55.2%, 38.1% and 6.7% for AA, AT and TT genotype, respectively. The frequency of the A allele was found as 74.3% and those of the T allele as 25.7%. The statistical evaluation represented that the allele frequencies was accordant with Hardy- Weinberg equilibrium (χ2 =0.002, p=0.97). This was the first study examining the frequencies of GLUT1 2841A>T polymorphism in Turkish population. In our opinion, these results can be used in epidemiological studies both in Turkiye and other countries.

Keywords

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Abstract

Glukoz transporteri (Glut1) hücrenin bazolateralinde yer almaktadır ve insulinden bağımsız olarak glukozun tek yönlü taşınımını sağlar. Özellikle kanser hücrelerine glukoz taşımasından sorumlu olan Glut1 ayrıca, diyabetle ilişkili olarak arsenik bileşiklerini de taşınmaktadır. Söz konusu çalışma, türk popülasyonundaki Glut1 (a2841t) tek nükleotit polimorfizminin allel ve genotip frekansını belirlemeyi amaçlamaktadır. Birbirinden bağımsız 100 erkek

Keywords

• Glukoz transporter 1, türk popülasyonu, polimorfizm, HpyCH4

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