Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines

Abstract

Laurocerasus officinalis is a popular black summer fruit and it belongs to Prunus genus and Rosaceae family. L. officinalis has many biological activities, such as anti-inflammatory, antinociceptive, antioxidant, neuroprotective, antidiabetic, among others. The aim of the current study was to determine the antioxidant activity and cytotoxic effects of dimethyl sulfoxide extract of L. officinalis (DEL). Total polyphenolic content and cytotoxic activity of DEL was investigated using Folin-Ciocalteu method and MTT assay, respectively. Total polyphenolic content of DEL was found 33.7±0.13 mg gallic acid equivalent/g fruit powder. DEL exhibited selective cytotoxic effect against four human cancer (lung, colon, liver and cervix) cell lines. The predominant selective cytotoxic effect of DEL was observed against colon cancer cells (IC₅₀=265.2±8.1 µg/mL) compared to normal fibroblast cells. These data demonstrate that L. officinalis extract exhibited selective cytotoxicity towards some cancer cells. To clarify the effective molecules and their mechanisms, further studies are needed to undertaken.

Keywords

• Antioxidant activity,Cherry laurel,Cytotoxic effect,Laurocerasus officinalis,Polyphenolic compounds

Laurocerasus officinalis Ekstraktının İnsan Kanser Hücre Serileri Üzerindeki Sitotoksik Etkisi

Abstract

Laurocerasus officinalis Roem. (Prunus laurocerasus L.), yerel  olarak karayemiş veya taflan olarak da bilinir, Prunus cinsi ve  Rosaceae familyasına mensup, koyu renkli bir yaz meyvesidir.  L. officinalis, anti-inflamatuvar, antinosiseptif, antioksidan,  nöroprotektif ve antidiyabetik gibi çeşitli biyolojik aktiviteler  göstermektedir. Bu çalışmanın amacı, L. officinalis’in dimetil  sülfoksitli ekstraktının antioksidan ve sitotoksik etkilerini  belirlemektir. Ekstraktın toplam polifenol içeriği Folin-  Ciocalteu metodu, sitotoksik etkinliği ise MTT yöntemiyle  belirlendi. Ekstraktın toplam polifenol içeriği 33.7±0.13 mg  gallik asit ekivalanı/g örnek olarak bulundu. Ekstrakt akciğer,  kolon, karaciğer ve serviks kanser hücre serilerinde seçici  bir sitotoksik etki gösterdi. Normal fibroblast hücreleri ile  karşılaştırıldığında en etkili seçici sitotoksik etkinin kolon  kanseri hücrelerinde olduğu belirlendi (IC50=265.2±8.1 μg/mL).  Sonuçlar L. officinalis ekstraktının bazı kanser hücre serilerinde  seçici bir sitotoksik etki gösterebildiğini ortaya koymaktadır.  Ekstrakttaki etken molekülleri ve bunların etki mekanizmalarını  belirleyebilmek için daha ileri çalışmalara ihtiyaç vardır.  

Keywords

• Antioksidan aktivite,Karayemiş,Laurocerasus officinalis,Sitotoksik etki,Polifenolik bileşikler

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