Asetamiprid ve d-Tübokürarin'in Kurbağa Sinir Dokusu Üzerine Etkilerinin İncelenmesi (I: Oksidatif Potansiyel)

Abstract

Bu çalışmada, asetamiprid ve d-tübokürarin’in Rana ridibunda  siyatik siniri üzerine oksidatif stres etkisinin incelenmesi  amaçlanmıştır. Bu amaçla kurulan deneylerde, 40 adet kurbağa  siyatik sinir preparatı kullanılmıştır. Kurbağalar, spinal hale  getirildikten sonra siyatik sinirler izole edilmiştir. Sinirlere,  120 dakika boyunca 1×10^-3, 1×10^-4, 1×10-5 ve 1×10^-6 M olmak  üzere dört farklı konsantrasyondaki asetamiprid çözeltileri  uygulanmıştır. Bunun yanı sıra siyatik sinir dokuları, 1x10^-3  M asetamiprid ile 1x10^-2 M d-tübokürarin karışımı, 1x10^-5  M asetamiprid ile 1x10^-4 M d-tübokürarin karışımı ve 1x10^-6  M asetamiprid ile 1x10^-5 M d-tübokürarin karışımında 120  dakika bekletilmiştir. Kontrol grubundaki sinir dokuları, 120  dakika süresince Ringer çözeltisinde bekletilmiştir. Tüm deney  gruplarında agonist ve antagonist etkileri eşit sayıda denek  üzerinde çalışılmıştır (N=5). Kurbağalarda oksidatif stresi  değerlendirmek için, katalaz (CAT) ve asetilkolinesteraz (AChE)  aktiviteleri ile malondialdehit (MDA) düzeyleri ölçülmüştür. 

Yapılan biyomarkır analizleri sonucunda, 1x10^-3 M  asetamiprid’in siyatik sinirde, biyokimyasal parametrelerden  CAT ve AChE aktivitesini kontrol grubuna göre önemli  biçimde azalttığı gözlenmiştir (P<0.05). Buna karşılık aynı  konsantrasyondaki insektisitin, sinir dokuda MDA seviyesini  kontrol grubuna göre önemli biçimde arttırdığı tespit edilmiştir  (p<0.05). 

Asetamiprid’in siyatik sinir dokusunda, biyokimyasal  parametrelerden CAT ile AChE’yi anlamlı şekilde düşürmesi,  MDA’yı ise önemli biçimde yükseltmesi, bu insektisitin yüksek  konsantrasyonda periferik sinirler üzerine oksidatif strese bağlı  hasar meydana getirdiğini göstermektedir.  

Keywords

• Asetamiprid,d-tübokürarin,sinir doku,oksidatif stres,kurbağa

Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential)

Abstract

The aim of this study is to investigate the oxidative stress effect  of acetamiprid and d-tubocurarine on the sciatic nerve of Rana  ridibunda by using biochemical methods. For this purpose, 40  frog sciatic nerve preparations were used in the experiments  established. Frog sciatic nerves were isolated after making  them spinal. Four different concentrations of acetamiprid  solution (1×10^-3, 1×10^-4, 1×10^-5 and 1×10^-6 M) were applied on  nerves for 120 minutes. In addition, the sciatic nerve tissues  were maintained for 120 minutes in the mixture of 1x10^-3 M  acetamiprid and 1x10-2 M d-tubocurarine, the mixture of 1x10^-5 M acetamiprid and 1x10^-4 M d-tubocurarine, the mixture of  1x10^-6 M acetamiprid and 1x10^-5 M d-tubocurarine. Nerve  tissues in the control group were maintained in Ringer’s solution  for 120 minutes. The agonist and antagonist effects were studied  in the experimental group and equal number of subjects (N =  5). Activity of catalase (CAT) and acetylcholinesterase (AChE)  as well as malondialdehyde (MDA) levels were measured to  evaluate oxidative stress in frogs. 

The results of the biomarker analysis revealed that the  application of 1x10^-3 M acetamiprid on sciatic nerve significantly  reduced the CAT and AChE activity compared to that of control  group (p<0.05). In contrast, it was determined that, the same  concentration of the insecticide significantly increased the level  of MDA on nerve tissue compared to that of control group  (p<0.05). 

The significant reduction of CAT and AChE and significant  increase of MDA in the sciatic nerve due to acetamiprid indicate  that this insecticide, depending upon the oxidative stress, causes  damage on peripheral nerves in high concentrations.  

Keywords

• Acetamiprid,d-tubocurarine,nerve tissue,oxidative stress,frog

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