Cellular toxicity and biological activities of honey bee (Apis mellifera L.) venom

Abstract

Bee venom (BV) has been suggested as an apitherapy tool

to be considered for various diseases including cancer.

However, the mechanisms action of BV and its toxicity on

tumorigenic and nontumorigenic cells are poorly understood.

Here, we investigated the antiproliferative, cytotoxic and

antibacterial activities of honey bee (Apis mellifera L.) venom

on nontumorigenic cells, several tumor cell lines and multidrug

resistant human pathogens (MDRP) such as Extended Spectrum

Beta-Lactamases producing Escherichia coli and Vancomycinresistant

Enterococcus Enterococcus faecium. BV treatment

showed significant antiproliferative, cytotoxic and antibacterial

activities. Our results suggest that BV is highly toxic not only

to cancer cell lines but also to nontumorigenic cell line as well.

We also investigated the mechanism action of BV, which caused

a cleavage of genomic DNA and inhibition of cell migration,

indicating induction of apoptosis. Immunohistochemistry

studies demonstrated that BV decreased the expression of

Bcl-2 and P16. BV showed antimicrobial activity against several

tested-MDRP. Our results indicate that clinic consideration of

BV for the treatment of malignancy needs to be re-evaluated

due to its cytotoxicity against normal cells.

Keywords

• Apis mellifera bee venom,Antiproliferative activity,Antibacterial activity,Cytotoxic activity

Cellular toxicity and biological activities of honey bee (Apis mellifera L.) venom

Abstract

Arı zehri kanser dahil çeşitli hastalıklar için bir apiterapi aracı

olarak önerilmektedir. Ancak, arı zehrinin etki mekanizması

ve tümörojenik ve tümörojenik olmayan hücreler üzerindeki

toksisitesi çok fazla anlaşılamamıştır. Biz burada bal arısı (Apis

mellifera L.) zehrinin tümörojenik olmayan, tümörojenik olan

kanser hücreleri ve Genişlemiş Spektrumlu Beta Laktamaz üreten

Escherichia coli and Vankomisin-dirençli Enterococcus faecium

gibi çoklu dirençli patojenler (MDRP) üzerinde antiproliferatif,

sitotoksik ve antibakteriyel aktivitesini araştırdık. Arı zehri

uygulaması önemli antiproliferatif, sitotoksik ve antibakteriyel

aktivite göstermiştir. Bizim sonuçlarımız arı zehrinin sadece

kanser hücrelerine değil nontümörojenik hücre hattına da

toksik olduğunu göstermektedir. Biz ayrıca etki mekanizmasını

araştırdığımız arı zehrinin apoptozisi işaret eden genomik DNA

kırılmasına ve hücre göçü inhibisyonuna neden olduğunu

gördük. İmmünokimyasal çalışmalar arı zehrinin Bcl-2 ve P16

ifadelerinde azalmaya neden olduğu göstermiştir. Arı zehri test

edilen bazı çoklu dirençli bakterilere karşı antimikrobiyal aktivite

göstermiştir. Bizim sonuçlarımız malignensilerin tedavisi için

arı zehrinin klinik değerlendirilmesinin normal hücrelere karşı

toksik etkisine bağlı olarak yeniden değerlendirilmesine ihtiyaç

olduğunu göstermektedir.

Keywords

• Apis mellifera arı zehiri,Antiproliferatif aktivite,Antibakteriyel etkinlik,sitotoksik aktivite

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