Biological activities of aerial parts extracts of endemic Tanacetum argenteum subsp. argenteum

Abstract

The objective of the present study was to investigate the

antimicrobial and anti-proliferative activities of n-hexane,

chloroform, methanol and aqueous methanol extracts obtained

from Tanacetum argenteum subsp. argenteum aerial parts. Antiproliferative

activity was tested in vitro against four human

cancer cell lines (A549: lung adenocarcinoma, Hela; cervix

adenocarcinoma, HT-29: colon adenocarcinoma, MCF-7; breast

adenocarcinoma) using MTT assay. Antimicrobial activity was

assessed by micro-broth dilution technique against Staphylococcus

aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228,

Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212,

Klebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa

ATCC 27853, Proteus mirabilis ATCC 14153, and Candida

albicans ATCC 10231. Hexane extract inhibited proliferation

of HT-29 and MCF-7 by 75 and 74% while chloroform extract

against the same cancer cell lines displayed inhibition of 89

and 73% at the concentration of 30 μg/mL, respectively. Also,

chloroform extract at the same concentration showed significant

anti-proliferative activity against A-549 and HeLa with inhibition

values of 75% and 62%. Chloroform extract exhibited moderate

antibacterial activity against Staphylococcus aureus and S.

epidermidis with the MIC values of 625 μg/mL. Methanol and

aqueous methanol extracts showed weak antimicrobial activity

against Staphylococcus epidermidis and Candida albicans with

MIC values of 1250 μg/mL. The results showed that n-hexane and

chloroform extracts have significant

Keywords

• Asteraceae,antimicrobial activity,anti-proliferative activity,n-hexane extract,chloroform extract

Endemik Tanacetum argenteum subsp. argenteum bitkisinin toprak üstü kısımlarından elde edilen ekstrelerin biyolojik aktiviteleri

Abstract

Bu çalışmanın amacı, Tanacetum argenteum subsp.

argenteum bitkisinin toprak üstü kısımlarından elde edilen

n-hekzan, kloroform, metanol ve sulu metanol ekstrelerinin

antimikrobiyal ve antiproliferatif aktivitelerini araştırmaktır.

Ekstrelerin antiproliferatif aktivitesi dört farklı insan kanser

hücre hatlarına (A549: Akciğer adenokarsinoma, Hela; Serviks

adenokarsinoma, HT-29: Kolon adenokarsinoma, MCF-7;

Meme adenokarsinoma) karşı MTT yöntemiyle test edilmiştir.

Ekstrelerin antimikrobiyal aktivitesi ise Staphylococcus aureus

ATCC 29213, Staphylococcus epidermidis ATCC 12228,

Escherichia coli ATCC 25922, Enterococcus faecalis ATCC

29212, Klebsiella pneumoniae ATCC 4352, Pseudomonas

aeruginosa ATCC 27853, Proteus mirabilis ATCC 14153, ve

Candida albicans ATCC 10231 suşlarına karşı mikro-broth

dilüsyon yöntemiyle araştırılmıştır. Kloroform ekstresi 30 μg/

mL konsantrasyonda HT-29 and MCF-7 hücre hatlarının

gelişimini sırasıyla % 89 ve % 73 oranında durdururken,

n-hekzan ekstresi ise aynı konsantrasyonda bu hücre hatlarının

gelişimini sırasıyla % 75 ve % 74 oranında inhibe etmiştir. Aynı

zamanda kloroform ekstresi A-549 and HeLa hücre hatlarının

gelişimi sırasıyla % 75 ve % 62 oranında durdurarak önemli

bir antiproliferatif aktivite göstermiştir. Kloroform ekstresi,

Staphylococcus aureus and S. epidermidis’e karşı 625 μg/

mL’lik MİK değerleriyle orta derecede antibakteriyel aktivite

göstermiştir. Metanol ve sulu methanol ekstreleri ise 1250

μg/mL’lik MİK değerleriyle Staphylococcus epidermidis ve

Candida albicans suşlarına karşı zayıf antimikrobiyal aktivite

göstermiştir. Bu sonuçlar, n-hekzan ve kloroform ekstresinin bu

çalışmada kullanılan insan kanser hücre dizilerine karşı önemli

bir antikanser aktiviteye sahip olduklarını gösterdi.

Keywords

• Asteraceae,antimikrobiyal aktivite,antiproliferatif aktivite,n-hekzan ekstresi,kloroform ekstresi

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