Investigation of Antioxidant and Enzyme Inhibition Activities and Phenolic Compound of Medicago rigidula (L.) All.

Abstract

The study focused on evaluating of antioxidant capacity,

phenolic compounds and enzyme inhibition activity of

methanolic extract Medicago rigidula. Antioxidant activity

was evaluated using different assays including 2,2-diphenyl-1-

pikrylhydrazyl (DPPH), ferric ion reducing antioxidant power

(FRAP), cupric ion reducing antioxidant capacity (CUPRAC)

and total antioxidant capacity (phosphomolybdenum) test.

Total phenolic and flavonoit contents were also determined.

Enzyme inhibition activities were examined against

anticholinesterase, tyrosinase, alpha-amylase and alphaglucosidase.

Phenolic content of M. rigidula included catechin,

cinnamic, chlorogenic, gallic and syringic acids. Free radical

scavenging activity of methanol extract was 20.30% at 1 mg/

mL concentration. In phosphomolybdenum and FRAP tests

were found 167.27 mg AAE/g and 67.01 mg TE/g, respectively.

Total phenolic and flavonoid contents were determined as 79.61

mg GAE/g and 27.38 μg QE/g, respectively. The results showed

that M. rigidula has a good source of antioxidants and enzyme

inhibition capacity. Thus, it can

Keywords

• Chemical composition,bioactivity,HPLC analysis,Fabaceae

Medicago rigidula (L.) ALL.’nın Antioksidan ve Enzim İnhibisyon Aktiviteleri ve Fenolik Bileşiminin İncelenmesi

Abstract

Çalışma Medicago rigidula (L.) All. metanol ekstresinin

antioksidan kapasitesi, fenolik bileşimi ve enzim inhibisyon

aktivitesinin değerlendirilmesi üzerine odaklanmıştır.

Antioksidan aktivite 2,2-difenill-1-pikrilhidrazil (DPPH),

demir indirgeme antioksidan gücü (FRAP), bakır iyonu

indirgeme gücü (CUPRAC) ve total antioksidan kapasite

(fosfomolibdat) testini içeren farklı yöntemler kullanılarak

değerlendirilmiştir. Aynı zamanda total fenolik ve flavonoit

içerikler de belirlenmiştir. Enzim inhibisyon aktiviteleri

antikolinesteraz, tirozinaz, alfa-amilaz ve alfa-glukozidaza

karşı incelenmiştir. M. rigidula bitkisinin fenolik bileşenlerinin

kateşin, sinnamik asit, klorojenik asit, gallik asit ve sirinjik asit

bileşikleri olduğu saptanmıştır. Metanol ekstresinin serbest

radikal giderici aktivitesi 1 mg/mL konsantrasyonda %20.30’dur.

Fosfomolibdat ve FRAP testlerinde sırası ile 167.27 mg AAE/g

ve 67.01 mg TE/g bulundu. Total fenolik ve flavonoit içerik

sırasıyla 79.61 mg GAE/g ve 27.38 μg QE/g olarak belirlenmiştir.

Sonuçlar M. rigidula’nın iyi bir antioksidan ve enzim inhibisyon

kapasitesine sahip olduğunu gösterdi. Bu nedenle Medicago

rigidula bitkisinin gıda ve ilaç endüstrisinde hammadde olarak

kullanılabileceği düşünülmektedir.

Keywords

• Kimyasal kompozisyon,biyoaktivite,HPLC analizi,Fabaceae

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