Evaluation of Relationship Between Lymphocyte DNA Damages and Blood Arsenic Levels in Silver Mining Workers using Alkaline Comet Assay

Abstract

The purpose of this study is to investigate the association

between DNA damage and blood arsenic levels in individuals

occupationally exposed to arsenic (As). A hundred-twenty

exposed individuals from silver mining plate workers were

monitored in the way of DNA damage in blood lymphocytes

using comet assay (single cell gel electrophoresis, SSGE). The

DNA damage levels were measured by BAB Bs Comet Assay

system and As levels were analyzed by the atomic absorption

spectrometer (AAS) system. The mean blood arsenic level was

17.03±10.85 μg/L. A statistically significant positive correlations

were found between the arsenic levels and comet parameters

(tail intensity, comet length and tail length) (r=0.360, r=0.334,

r=0.259, respectively, p<0.01). Our results showed that exposure

to arsenic may cause DNA damage in peripheral

Keywords

• DNA damage; blood arsenic levels; Turkish workers; comet assay

Gümüş Madeni İşçilerinde Kan Arsenik Düzeyi ve Lenfosit DNA hasarı Arasındaki İlişkinin Alkali Komet Analizi ile Değerlendirilmesi

Abstract

Bu çalışmadakı amaç, mesleki arseniğe maruz bireylerde

kan arsenik (As) düzeyi ve DNA hasar arasındaki ilişkiyi

araştırmaktır. Gümüş madeni işçilerinden 120 bireyin

lenfositlerindeki DNA hasarı comet yöntemi (tek hücre jel

elektroforezi) kullanılarak belirlendi. DNA hasar düzeyleri BAB

Bs Comet Assay sistemi ile hesaplandı ve As düzeyleri atomic

absorbsiyon spektroskopi (AAS) cihazı ile analiz edildi. Kan As

düzeyi ortalaması 17.03±10.85 μg/L olarak tespit edildi. Kan As

ve comet parametreleri (kuyruk yoğunluğu, comet uzunluğu ve

kuyruk uzunluğu) arasında istatistiksel olarak anlamlı pozitif

korelasyon bulundu (sırasıyla; r=0.360, r=0.334, r=0.259;

p<0.01). Sonuçlarımız, Türk Eti Maden gümüş işçilerinde

arsenik maruziyetinin periferik lenfosit DNA hasarına sebep

olduğunu gösterdi.

Keywords

• DNA hasarı,kan arsenik düzeyi,Türk işçiler,komet yöntemİ

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