The effect of silymarin on the liver in thermal burn injury

Yıl 2014, Cilt: 18 Sayı: 2, 56 - 61, 16.05.2014

Evren Yemişen Ayşen Yarat Tuğba Tunalı Akbay Hale Toklu Göksel Şener

Öz

Hepatic homeostasis and metabolism are essential for survival
in critically ill and severely burned patients. Silymarin,
the major component of thistle milk has been shown to have
hepatoprotective effect. In this study the effect of silymarin
on the liver of burned rats was investigated. Wistar Albino
rats were exposed to 900C bath for 10 seconds to induce skin
burn. Silymarin either locally (30 mg/kg) applied on 4 cm2
area or locally+systemically (50 mg/kg p.o) was administered
after burn and repeated twice daily. Rats were decapitated
48 h after injury and blood was collected for tumor necrosis
factor-α (TNF- α) and lactate dehydrogenase (LDH)
activity. In liver tissue samples, total protein, tissue factor
activity (TF) and activities of carbonic anhydrase (CA), glutathione-
S-transferase (GST), catalase (CAT), superoxide
dismutase (SOD), alkalen and acid phosphatase (ALP, ACP
respectively) enzymes were determined. In addition polyacyrlamid
gel electrophoresis was carried on liver tissue samples.
Burn caused a significant increase in blood TNF- α and
LDH levels. Total protein levels and ALP activity increased
and SOD activity decreased in the liver tissue at 48h
after burn. Both local and systemic silymarin treatment
significantly reversed this parameters except ALP. Silymarin
treatment significantly increased ALP activity. Local
silymarin treatment increased, CAT and ACP activities and
decreased TF activity compared to control and burn group;
and increased CA activity compared to systemic+local silymarin
treatment. Addition of systemic silymarin treatment
to local silymarin treatment reversed these effects of local
treatment to control group levels. Non significant differences
were found between protein bands obtained in electrophoresis.
Minor liver damage was obvious 48 h after thermal
skin burn. Both local and systemic silymarin treatment
were effective to reverse the effects of burn induced liver
injury.
Key Words: Burn, liver enzymes, liver tissue factor activity,
SDS-polyacrylamide gel electrophoresis, silymarin

Anahtar Kelimeler

Burn, liver enzymes, liver tissue factor activity, SDS-polyacrylamide gel electrophoresis, silymarin

ORIGINAL RESEARCH

Öz

Karaciğer yanık hastaları için hayati önem taşır. Meryamana dikeninin ana maddesi olan silmarinin karaciğer üzerindeki koruyucu etkisi gösterilmiştir. Bu çalışmada termal deri yanıklı sıçanlarda silmarinin karaciğer üzerine etkisi araştırıldı. WistarAlbino türü sıçanlar 90oC deki su ile 10 saniye temas ettirilerek deri yanığı oluşturuldu. Silmarin hem lokal (30 mg/kg) olarak hemde lokal + oral yolla (50 mg/kg) yanık oluşturulduktan sonra 2 gün (48 saat) süre ile günde 2 kez uygulandı. 48 saat sonunda sıçanlar dekapite edildi ve kan örneklerinde tümör nekroz faktör (TNF-α) ve laktat dehidrogenaz (LDH) aktivitesi incelendi, karaciğer dokusunda protein, doku faktörü (DF) aktivitesi, karbonik anhidraz (CA), glutatyon-S-transferaz (GST), katalaz (CAT), süperoksid dismutaz (SOD), alkalen fosfataz (ALP), asit fosfataz (ACP) gibi enzim aktiviteleri tayin edildi. Ayrıca SDS

Anahtar Kelimeler

Karaciğer doku faktörü aktivitesi, Karaciğer enzimleri, SDS-poliakrilamid jel elektroforezi, Silmarin, Yanık

Kaynakça

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