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Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae)

Year 2014, Volume: 18 Issue: 3, 135 - 138, 12.09.2014

Abstract

The aim of present study was to investigate the anti-diarrheal

activity of Ficus microcarpa against experimentally induced

diarrhea in rats. Albino rats were used for the experiment.

Anti-diarrheal activity was determined by castor oil induced

diarrhea. The extract of the bark administered orally at a

dose 300 mg/kg and 600 mg/kg. The extract of Ficus

microcarpa produced a marked anti-diarrheal effect in rats.

Both doses of extract significantly decreased (P<0.05) the

total number of wet feces produced by administration of

castor oil (4.31 at the dose of 300 mg/kg and 7.16 at the dose

of 600 mg/kg) as compared to the castor oil treated control

group (20.75). The Ficus microcarpa extract was found to

possess an anti-enteropooling activity. Oral administration of

castor oil produced a significant increase (P<0.05) in the

intestinal fluid (3.14 ml) as compared to the normal rat (1.29

ml). The extract when given orally one hour before castor

oil, significantly inhibited (P<0.05) the enteropooling; 1.48

ml (300mg/kg) and 1.68 ml (600mg/kg). The results revealed

that the extract inhibited the small intestinal motility of the

charcoal marker in the rats by 23.92-31.12% whereas the

inhibition was noted to be 61.2% in the case of treatment by

loperamide. The results of this investigation conclude that

the extract contains pharmacologically active substances

with anti-diarrheal properties. Further research is to be

carried out to fractionate and purify the extract, in order to

find the molecules responsible for the anti-diarrheal activity.

Keywords: Anti-diarrheal activity, Ficus Microcarpa, Castor

oil, Methanolic extract.

References

  • Mani M, Sachan N, Tandon A, Wahi AK. Anti-diarrheal activity of methanolic extract of root bark of Ailanthus altissima Swingle (Family: Simaroubaceae) on experimental animals. Int J Pharm Sci Res 2010;1:197-202.
  • Park K. Park’s Textbook of preventive and social medicine. Jabalpur, India: M/S Banarsidas Bharat Publishes; 2000;172-5.
  • Mohammed A, Ahmed H, Goji ADT, Okpanachi AO, Ezekiel I, Tanko Y. Preliminary Anti-diarrheal Activity of Hydromethanolic Extract of Aerial Part of Indigofera pulchra in Rodents. Asian J Med Sci; 2009; 1: 22-5.
  • Wagner WL, Herbst DR, Sohmer SH. Manual of the Flowering Plants of Hawaii. 2 nd ed. Honolulu: University of Hawaii and Bishop Museum Press; 1999.
  • Warrier PK, Nambiar VPK, Ramankutty C. Indian Medicinal Plants a Compendium of 500 Species.3 rd ed. Madras: Orient Longman; 1995.
  • Chiang YM, Chang JY, Kuo CC. Cytotoxic triterpenes from the aerial roots of Ficus microcarpa. J Phytochemistry 2005; 66: 495-501.
  • Taira T, Ohdomari A, Nakama N,Shimoji M, Ishihara M. Characterization and antifungal activity of gazyumaru (Ficus microcarpa) latex chitinase: Both the chitin-binding and the antifungal activities of class 1 chitinase are reinforced with increasing ionic strength. Biosci Biotechnol Biochem 2005; 69: 811-8.
  • Nasrin A, Iran R, Amir M. Hepatoprotective Activity of Capparis spinosa Root Bark Against CCl4 Induced Hepatic Damage in Mice. Iranian Journal of Pharmaceutical Research 2007; 6: 285-90.
  • Trease G.E. and W.C. Evans. Textbook of Pharmacognosy. 15th Edn. London: Bailliere Tindal;2006,135-9.
  • Hilaly JE, Israili Z.H, Lyoussi B. Acute and chronic toxicological studies of Ajuga iva in experimental animals. J Ethnopharmacol 2004; 91: 43-50.
  • Doherty SS. Inhibition of arachinodic acid release, mechanism by which glucocorticoids inhibit endotoxin-induced diarrhea. British J Pharmacol 1981; 73: 549-54.
  • Zaval MA, Pera ZS, Perez P, Vargan R, Perz RM. Antidiarrheal activity of Waltheria anorlana, Commelina coelestis and Alternanthera repens. J Ethnopharmacol 1988; 61: 41- 7.
  • Robert A, Nezamis JF, Lancaster C, Hanchar AJ, Klepper MS. Enteropooling assay: a test for diarrhea produced by prostaglandins. Prostaglandins 1976; 11:809-28.
  • Rao VSN, Santos FA, Sabreira TT, Souza MF, Melo CL, Silveria ER. Investigations on the gastroprotective and antidiarrhoeal properties of ternatin, a tetramethoxyflavone from Egletes viscosa. Planta Medica 1997;63:146-9
  • Ammon PJ, Thomas, Philips S. Effects of oleic acid and ricinolic net jejuna water and electrolyte movement. J Clin Invest 1974; 53:374-9. Tripathi KD. Essentials of Medical Pharmacology. New Delhi: Jaypee Brothers Medical Publishers (P); 1994.

ORIGINAL RESEARCH

Year 2014, Volume: 18 Issue: 3, 135 - 138, 12.09.2014

Abstract

Bu çalışmanın amacı, sıçanlarda deneysel olarak oluşturulan diyareye karşı Ficus microcarpa ekstresinin antidiyareik etkinliğini araştırmaktır. Deneylerde Albino sıçanlar kullanıldı. Antidiyareik aktivite hint yağı ile oluşturulan diyare modelinde çalışıldı. 300 mg/kg ve 600 mg/kg dozlarında Ficus microcarpa ekstresi sıçanlarda anlamlı bir anti diyareik etki gösterdi. Toplam ıslak dışkı sayısı hint yağı grubuna (20.75) göre ekstre tedavisi uygulanan gruplarda her iki dozda önemli ölçüde (P <0.05; 300 mg/kg ‘de 4.31 ve 600 mg/kg dozunda 7.16) azaldı. Ficus microcarpa ekstresinin anti- enteropooling etkinliğe sahip

References

  • Mani M, Sachan N, Tandon A, Wahi AK. Anti-diarrheal activity of methanolic extract of root bark of Ailanthus altissima Swingle (Family: Simaroubaceae) on experimental animals. Int J Pharm Sci Res 2010;1:197-202.
  • Park K. Park’s Textbook of preventive and social medicine. Jabalpur, India: M/S Banarsidas Bharat Publishes; 2000;172-5.
  • Mohammed A, Ahmed H, Goji ADT, Okpanachi AO, Ezekiel I, Tanko Y. Preliminary Anti-diarrheal Activity of Hydromethanolic Extract of Aerial Part of Indigofera pulchra in Rodents. Asian J Med Sci; 2009; 1: 22-5.
  • Wagner WL, Herbst DR, Sohmer SH. Manual of the Flowering Plants of Hawaii. 2 nd ed. Honolulu: University of Hawaii and Bishop Museum Press; 1999.
  • Warrier PK, Nambiar VPK, Ramankutty C. Indian Medicinal Plants a Compendium of 500 Species.3 rd ed. Madras: Orient Longman; 1995.
  • Chiang YM, Chang JY, Kuo CC. Cytotoxic triterpenes from the aerial roots of Ficus microcarpa. J Phytochemistry 2005; 66: 495-501.
  • Taira T, Ohdomari A, Nakama N,Shimoji M, Ishihara M. Characterization and antifungal activity of gazyumaru (Ficus microcarpa) latex chitinase: Both the chitin-binding and the antifungal activities of class 1 chitinase are reinforced with increasing ionic strength. Biosci Biotechnol Biochem 2005; 69: 811-8.
  • Nasrin A, Iran R, Amir M. Hepatoprotective Activity of Capparis spinosa Root Bark Against CCl4 Induced Hepatic Damage in Mice. Iranian Journal of Pharmaceutical Research 2007; 6: 285-90.
  • Trease G.E. and W.C. Evans. Textbook of Pharmacognosy. 15th Edn. London: Bailliere Tindal;2006,135-9.
  • Hilaly JE, Israili Z.H, Lyoussi B. Acute and chronic toxicological studies of Ajuga iva in experimental animals. J Ethnopharmacol 2004; 91: 43-50.
  • Doherty SS. Inhibition of arachinodic acid release, mechanism by which glucocorticoids inhibit endotoxin-induced diarrhea. British J Pharmacol 1981; 73: 549-54.
  • Zaval MA, Pera ZS, Perez P, Vargan R, Perz RM. Antidiarrheal activity of Waltheria anorlana, Commelina coelestis and Alternanthera repens. J Ethnopharmacol 1988; 61: 41- 7.
  • Robert A, Nezamis JF, Lancaster C, Hanchar AJ, Klepper MS. Enteropooling assay: a test for diarrhea produced by prostaglandins. Prostaglandins 1976; 11:809-28.
  • Rao VSN, Santos FA, Sabreira TT, Souza MF, Melo CL, Silveria ER. Investigations on the gastroprotective and antidiarrhoeal properties of ternatin, a tetramethoxyflavone from Egletes viscosa. Planta Medica 1997;63:146-9
  • Ammon PJ, Thomas, Philips S. Effects of oleic acid and ricinolic net jejuna water and electrolyte movement. J Clin Invest 1974; 53:374-9. Tripathi KD. Essentials of Medical Pharmacology. New Delhi: Jaypee Brothers Medical Publishers (P); 1994.
There are 15 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Shripad Baıragı

Abhijeet Aher This is me

Nitin Nema This is me

Inayat Pathan This is me

Publication Date September 12, 2014
Published in Issue Year 2014 Volume: 18 Issue: 3

Cite

APA Baıragı, S., Aher, A., Nema, N., Pathan, I. (2014). Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae). Marmara Pharmaceutical Journal, 18(3), 135-138. https://doi.org/10.12991/mpj.2014187240
AMA Baıragı S, Aher A, Nema N, Pathan I. Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae). J Res Pharm. November 2014;18(3):135-138. doi:10.12991/mpj.2014187240
Chicago Baıragı, Shripad, Abhijeet Aher, Nitin Nema, and Inayat Pathan. “Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae)”. Marmara Pharmaceutical Journal 18, no. 3 (November 2014): 135-38. https://doi.org/10.12991/mpj.2014187240.
EndNote Baıragı S, Aher A, Nema N, Pathan I (November 1, 2014) Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae). Marmara Pharmaceutical Journal 18 3 135–138.
IEEE S. Baıragı, A. Aher, N. Nema, and I. Pathan, “Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae)”, J Res Pharm, vol. 18, no. 3, pp. 135–138, 2014, doi: 10.12991/mpj.2014187240.
ISNAD Baıragı, Shripad et al. “Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae)”. Marmara Pharmaceutical Journal 18/3 (November 2014), 135-138. https://doi.org/10.12991/mpj.2014187240.
JAMA Baıragı S, Aher A, Nema N, Pathan I. Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae). J Res Pharm. 2014;18:135–138.
MLA Baıragı, Shripad et al. “Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae)”. Marmara Pharmaceutical Journal, vol. 18, no. 3, 2014, pp. 135-8, doi:10.12991/mpj.2014187240.
Vancouver Baıragı S, Aher A, Nema N, Pathan I. Evaluation of Anti-Diarrhoeal Activity of the Leaves Extract of Ficus Microcarpa L. (Moraceae). J Res Pharm. 2014;18(3):135-8.

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