Screening for antioxidant and antimutagenic properties of extracts from Centaurea pterocaula as well as theirs enzyme inhibitory potentials

Year 2016, Volume: 20 Issue: 3, 232 - 242, 28.03.2016

Ahmet Uysal , Gokhan Zengin , Yusuf Durak , Abdurrahman Aktumsek

Abstract

The purpose of this study was to evaluate the antioxidant capacities, mutagenic/antimutagenic propeties and enzyme inhibitory effects of three extracts (ethyl acetate, methanol and water) of Centaurea pterocaula. The antioxidant properties were investigated using in vitro antixidant methods such as radical scavenging (DPPH assay), reducing power (FRAP and CUPRAC assays), phosphomolybdenum and metal chelating. Enzyme inhibitory effects were tested aganist cholinesterase, tyrosinase, amylase and glucosidase. Ames test was used to show mutagenic/antimutagenic properties of these extracts. Generally, methanol and ethyl acetate extracts had stronger activities as compared to water extract. Also, ethyl acetate extract exhibited the strongest effect on the tested enzymes (except for glucosidase) Total phenolic and flavonoid content were found to be 15.77-25.22 mgGAE/g extract and 0.67-31.44 mgRE/g extract, respectively. The mutagenicity was not seen for all extracts tested, while it was determined that some samples have significant antimutagenicity. In the condition of presence of S9 mix, ethyl acetate and methanol extracts revealed excellent antimutagenic activity (92% and 92%) at a dose of 5000 µg/plate against 2-aminoflourene (2-AF) for TA98 strain. According to the results of present study, C. pterocaula can be considered as a potential source for developing new nutraceuticals or pharmaceuticals.

Keywords

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