Cytotoxicity of triterpenes from the leaves of Garcinia prainiana King (Guttiferae)

Yıl 2017, Cilt: 21 Sayı: 1, 127 - 131, 15.08.2016

SHAMSUL On WAN MOHD NUZUL HAKIMI WAN Salleh FAREDIAH Ahmad HASNAH MOHD Sırat MUHAMMAD Taher

https://doi.org/10.12991/marupj.259890

Öz

Phytochemical investigation of the leaves of Garcinia prainiana resulted in the isolation of five triterpenes identified as squalene 1, friedelin 2, lupeol 3, 3β-hydroxyeupha-5,22-diene 4, and 3β-acetoxyeupha-5,22-diene 5. The structures of these compounds were established by analysis of their spectroscopic data, as compared to that of reported compounds. All compounds were screen for cytotoxicity against H1299 and A549 lung cancer cell lines using MTT assay. Compound 5 exhibited the most significant activity against H1299 and A549 cell lines with IC₅₀ values 18.0 µg/ml and 36.3 µg/ml, respectively.  

Anahtar Kelimeler

Triterpene, Garcinia prainiana, Cytotoxicity

Garcinia prainiana King (Guttiferae) Yapraklarından Elde Edilen Triterpenlerin Sitotoksik Özellikleri

Öz

Garcinia prainiana yapraklarını
konu alan fitokimyasal  araştırmalar
sonucunda triterpen yapısında beş bileşik izole 
edilmiştir; skualen 1, friedelin 2, lupeol 3, 3β-hidroksiöfa-  5,22-dien 4, and 3β-asetoksiöfa-5,22-dien 5.
İzole edilen  bileşiklerin yapıları
spektroskopik yöntemler kullanılarak  aydınlatılmış
ve elde edilen sonuçlar yukarıda belirtilen 
bileşiklere ait spektroskopik verilerle karşılaştırılmıştır. Tüm  bileşiklerin, H1299 ve A549 akciğer kanseri
hücre hatları  üzerindeki sitotoksik
etkileri MTT yöntemiyle çalışılmıştır.  3β-Asetoksiöfa-5,22-dien
5, H1299 ve A549 hücre hatlarına  karşı
sırasıyla IC50 = 18.0 μg/ml ve IC50= 36.3 μg/ml derişimde  etki göstererek serisindeki en etkili bileşik
olarak öne çıkmıştır.  

Anahtar Kelimeler

Triterpen, Garcinia prainiana, Sitotoksisite

Kaynakça

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