Asetamiprid ve d-Tübokürarin'in Kurbağa Sinir Dokusu Üzerine Etkilerinin İncelenmesi (I: Oksidatif Potansiyel)

Yıl 2017, Cilt: 21 Sayı: 1, 149 - 155, 15.08.2016

Yusuf Çamlıca Salih Cüfer Bediz Serap Yalın

https://doi.org/10.12991/marupj.259892

Öz

Bu çalışmada, asetamiprid ve
d-tübokürarin’in Rana ridibunda  siyatik
siniri üzerine oksidatif stres etkisinin incelenmesi  amaçlanmıştır. Bu amaçla kurulan deneylerde,
40 adet kurbağa  siyatik sinir preparatı
kullanılmıştır. Kurbağalar, spinal hale  getirildikten
sonra siyatik sinirler izole edilmiştir. Sinirlere,  120 dakika boyunca 1×10^-3, 1×10^-4, 1×10-5 ve
1×10^-6 M olmak  üzere dört farklı
konsantrasyondaki asetamiprid çözeltileri 
uygulanmıştır. Bunun yanı sıra siyatik sinir dokuları, 1x10^-3  M asetamiprid ile 1x10^-2 M d-tübokürarin
karışımı, 1x10^-5  M asetamiprid ile
1x10^-4 M d-tübokürarin karışımı ve 1x10^-6 
M asetamiprid ile 1x10^-5 M d-tübokürarin karışımında 120  dakika bekletilmiştir. Kontrol grubundaki
sinir dokuları, 120  dakika süresince
Ringer çözeltisinde bekletilmiştir. Tüm deney 
gruplarında agonist ve antagonist etkileri eşit sayıda denek  üzerinde çalışılmıştır (N=5). Kurbağalarda
oksidatif stresi  değerlendirmek için,
katalaz (CAT) ve asetilkolinesteraz (AChE) 
aktiviteleri ile malondialdehit (MDA) düzeyleri ölçülmüştür. 

Yapılan biyomarkır analizleri
sonucunda, 1x10^-3 M  asetamiprid’in
siyatik sinirde, biyokimyasal parametrelerden 
CAT ve AChE aktivitesini kontrol grubuna göre önemli  biçimde azalttığı gözlenmiştir (P<0.05).
Buna karşılık aynı  konsantrasyondaki
insektisitin, sinir dokuda MDA seviyesini 
kontrol grubuna göre önemli biçimde arttırdığı tespit edilmiştir  (p<0.05). 

Asetamiprid’in siyatik sinir
dokusunda, biyokimyasal  parametrelerden
CAT ile AChE’yi anlamlı şekilde düşürmesi, 
MDA’yı ise önemli biçimde yükseltmesi, bu insektisitin yüksek  konsantrasyonda periferik sinirler üzerine
oksidatif strese bağlı  hasar meydana
getirdiğini göstermektedir.  

Anahtar Kelimeler

Asetamiprid, d-tübokürarin, sinir doku, oksidatif stres, kurbağa

Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential)

Öz

The aim of this study is to
investigate the oxidative stress effect  of
acetamiprid and d-tubocurarine on the sciatic nerve of Rana  ridibunda by using biochemical methods. For
this purpose, 40  frog sciatic nerve
preparations were used in the experiments 
established. Frog sciatic nerves were isolated after making  them spinal. Four different concentrations of
acetamiprid  solution (1×10^-3, 1×10^-4,
1×10^-5 and 1×10^-6 M) were applied on  nerves
for 120 minutes. In addition, the sciatic nerve tissues  were maintained for 120 minutes in the
mixture of 1x10^-3 M  acetamiprid and
1x10-2 M d-tubocurarine, the mixture of 1x10^-5 M acetamiprid and 1x10^-4 M d-tubocurarine, the mixture of  1x10^-6 M acetamiprid and 1x10^-5 M
d-tubocurarine. Nerve  tissues in the
control group were maintained in Ringer’s solution  for 120 minutes. The agonist and antagonist
effects were studied  in the experimental
group and equal number of subjects (N =  5).
Activity of catalase (CAT) and acetylcholinesterase (AChE)  as well as malondialdehyde (MDA) levels were
measured to  evaluate oxidative stress in
frogs. 

The results of the biomarker
analysis revealed that the  application
of 1x10^-3 M acetamiprid on sciatic nerve significantly  reduced the CAT and AChE activity compared to
that of control  group (p<0.05). In
contrast, it was determined that, the same 
concentration of the insecticide significantly increased the level  of MDA on nerve tissue compared to that of
control group  (p<0.05). 

The significant reduction of CAT
and AChE and significant  increase of MDA
in the sciatic nerve due to acetamiprid indicate  that this insecticide, depending upon the
oxidative stress, causes  damage on
peripheral nerves in high concentrations.  

Anahtar Kelimeler

Acetamiprid, d-tubocurarine, nerve tissue, oxidative stress, frog

Kaynakça

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