Investigation of Antioxidants’s Antimutagenic Effects by The Ames Test

Year 2017, Volume: 21 Issue: 3, 455 - 460, 18.04.2017

Ayfer Beceren , Betül Sarıkaya Eşref Tatlıpınar Gülden Z Omurtag Semra Şardaş

https://doi.org/10.12991/marupj.306790

Abstract

Nowadays, several test systems have been developed in order

to observe the mutagenic effects of chemical agents which play

crucial roles in human health. The Ames Test is one of these test

systems. With the Ames Test, some bacterial mutants have been

discovered to investigate the mutagenic effects of the chemicals.

Various strains of Salmonella typhimurium are one of the groups

of the bacterial mutants in question. The aim of this study is to

investigate possible antimutagenic effect of Pelargonium sidoides

which have an antioxidant effect towards carcinogenic substance

called 2-aminofluorene by Ames/Salmonella/Microsome test

kit in the absence and presence of metabolic activation. TA 98

and TA 100 strains were used in these experiments. TA 98 is

designed for frame-shift mutagens and TA 100 is designed for

base-pair mutagens. The antimutagenic activity was screened in

two groups with or without S9 metabolic activation. The results

were evaluated the mean average values and compared with

positive and negative controls. In conclusion, it was shown that

Pelargonium sidoides have antimutagenic effect towards TA 98

and TA 100 without S9 metabolic activation (p≥0.05) but have

no antimutagenic effect towards

Keywords

Ames test, antioxidant, Pelargonium sidoides, S9 fraction, mutagenicity

Antioksidan Maddelerin Mutajenite Üzerine Etkilerinin Ames Testi ile İncelenmesi

Abstract

Günümüzde insan sağlığında önemli rol oynayan kimyasal

ajanların mutajenik etkilerinin gözlem altına alınmasını sağlayan

çeşitli test sistemleri geliştirilmiştir. Bu sistemlerden biri de

Ames testidir. Ames testi ile kimyasalların mutajenik etkilerinin

belirlenmesi için çeşitli mutant bakteriler geliştirilmiştir. Bunun

için de Salmonella typhimurium bakterisinin çeşitli suşları

kullanılmaktadır. Bu çalışmada Salmonella/mikrozom Ames

test kiti ile metabolik aktivasyon varlığında ve yokluğunda,

gen mutasyonlarına sebep olan karsinojenik madde olan

2-Aminoflorene karşı antioksidan etkiye sahip Pelargonium

sidoides’in muhtemel antimutajenik etkisinin araştırılması

amaçlanmıştır. Yöntemde Salmonella typhimurium TA 100 ve

TA 98 suşları ile çalışılmıştır. TA 100 baz çifti değişimine, TA

98 ise çerçeve kaymasına yol açan mutajenlerin belirlenmesi

için kullanılmıştır. Deneyler S9’lu ve S9’suz olmak üzere iki

grup halinde gerçekleştirilmiştir. Test sonuçlarının ortalaması

alınarak, pozitif ve negatif kontrol grupları ile karşılaştırılmış ve

değerlendirilmiştir. Çalışmamızda, Pelargonium sidoides’in TA

100 ve TA 98 suşları ile S9 yokluğunda 2-aminofluoren’e karşı

antimutajenik aktiviteye sahip olduğu (p≥0.05) S9 varlığında ise

antimutajenik aktiviteye sahip olmadığı (p≤0.05) gösterilmiştir.

Keywords

Ames Testi, antioksidan, Pelargonium sidoides, S9 fraksiyonu, mutajenisite

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