A validated HPLC method for simultaneous estimation of Melatonin and Octyl Methoxycinnamate in combined pharmaceutical applications

Yıl 2017, Cilt: 21 Sayı: 4, 921 - 930, 01.12.2017

Gülçin Arslan Azizoğlu Erkan Azizoğlu Sakine Tuncay Tanrıverdi Özgen Özer

Öz

A simple, fast and precise reverse phase high performance

liquid chromatographic method has been developed for

the simultaneous determination of Melatonin and Octyl

Methoxycinnamate. Melatonin has become an attractive

substrate in sunscreen formulations because of its high

antioxidant and photo-protection properties. Octyl

methoxycinnamate is one of the chemical UV filter that can be

found most of the sunscreen formulations up to 7.5 % according

Food and Drug Administration. The aim of the present study

was to develop and validate a High-Performance Liquid

Chromatography method for the determination of Melatonin

and Octyl Methoxycinnamate in combined pharmaceutical or

cosmetic applications. As a model of combined pharmaceutical

applications, a microemulsion consisting of Melatonin and

Octyl Methoxycinnamate was also prepared and characterized

in terms of droplet size, pH and viscosity. The separation was

performed with a Waters XTerra RP C18 (5 μm, 4.6 x 150 mm).

All HPLC assays were performed with 10 μl injection volume,

mobile phase consisting of acetonitrile and water, using gradient

elution starting at 20% and ending at 90% of acetonitrile with

a flow rate of 1.5 ml min-1.The eluent was monitored with

UV detection at 222 nm for Melatonin and 306 nm for Octyl

Methoxycinnamate. The method was validated according

to ICH guidelines. Validation parameters were specificity,

accuracy, precision (repeatability and reproducibility), linearity,

limit of detection (LOD) and limit of quantification (LOQ).

Analytical method development results indicated that the LOD

values were 0.132 and 0.049 μg/ml; LOQ values were 0.4 and

0.15 μg/ml and assay exhibited a linear range of 0.5- 60 μg/ml

for Melatonin and Octyl Methoxycinnamate, respectively.

Anahtar Kelimeler

Melatonin (PubChem CID: 896); Octyl methoxycinnamate (PubChem CID: 5355130) ; RP-HPLC method development.

Kaynakça

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