Lead bioaccumulation in gill, muscle and hepatopancreas tissues of Mediterranean green crab

Abstract

This study was conducted to determine lead bioaccumulation in gill, muscle and hepatopancreas tissues of the Mediterranean Green Crab, Carcinus aestuarii (Nardo, 1947) which exposed to sublethal concentrations of lead in vivo effect for 14 days. Lead accumulation in tissues of C.aestuarii was observed as gill>muscle>hepatopancreas in the end of 7th day and as gill>hepatopancreas>muscle in the end of 14th day. As a result of test, it was found that lead bioaccumulation in tissues of C. aestuarii that was exposed to different concentrations of lead (0.025; 0.05; 0.1 and 1.0 mg/L) was increased in line with the ambient lead concentration of and time of exposure. In addition, the most accumulation was determined in gills.

Keywords

• Carcinus aestuarii
• Lead
• Bioaccumulation

References

1. Anonymous, 1994. Microwave Assisted Acid Digestion of Sediments, Sludges, Soils, Andoils. Method EPA. 3051, Washington.
2. Bagatto, G., and M.A. Alikhan. 1987. Copper, Cadmium and Nickel Accumulation in Crayfish Populations Near Copper-Nickel Smelters at Subbury, Ontario, Canada. Bulletin of Environmental Contamination and Toxicology, 38: 540–545.
3. Barrento, S., Marques, A., Teixeira, B., Carvalho, M.L., Vaz-Pires, P., and M.L. Nunes. 2009. Accumulation of Elements (S, As, Br, Sr, Cd, Hg, Pb) in Two Populations of Cancer pagurus: Ecological Implications to Human Consumption. Food and Chemical Toxicology, 47: 150–156.
4. Bondgaard, M., Norum, U., and P. Bjerregaard. 2000. Cadmium Accumulation in the Female Shore Crab Carcinus maenas During the Moult Cycle and Ovarian Maturation. Marine Biology, 137: 995–1004.
5. Bryan, G.W. 1968. Concentranions on Zinc and Copper in Tissues of Decapod Crustaceans. Journal of the Marine Biological Association of the United Kingdom, 48: 303–321.
6. Bu-Olayan, A.H., and M.N.V. Subrahmanyam. 1998. Trace Metal Concentrations in the Crab Macrophthalmus depressus and Sediments on the Kuwait Coast. Environmental Monitoring and Assessment, 53: 297–304.
7. Cairns, J. Jr., and Jr. Pratt. 1993. A History of Biological Monitoring Using Benthic Macro Invertebrates. In: Roesenberg D.M. and Resh V.H. (Eds), Freshwater Biomonitoring and Benthic Macro Invertebrates. Chapman and Hall. 10-27.
8. Canlı, M., and R.M. Stagg. 1996. The Effect of in vivo Exposure to Cadmium, Copper and Zinc on The Activities of Gill ATPase in The Norway Lobster Nephrops norvegicus. Bulletin of Environmental Contamination and Toxicology, 31: 494–501.

9. Cinier, C. C., Petit-Ramel, M., Faure, R., Garin, D., and Y. Bouvet. 1999. Kinetics of Cadmium Accumulation and Eliminination in Carp Cyprinus carpio Tissues. Comparative Biochemistry and Physiology, 122: 345-352.
10. Heath, A. G. 1987. Water Pollution and Fish Physiology. CRC., Florida, USA. 245.
11. Hopkin, S. P., and J. A. Nott. 1979. Some Observations on Concentrically Structured Intracellular Granules in the Hepatopancreas of the Shore Crab Carcinus maenas. Journal of the Marine Biological Association of the United Kingdom, 59:867.
12. Ip, C. C. M., Li, X. D., Zhang, G., Wong, C. S. C., and W. L. Zhang. 2005. Heavy Metal and Pb Isotopic Compositions of Aquatic Organisms in the Pearl River Estuary, South China. Environmental Pollution, 138: 495-505.
13. Kalay, M., and M. Canlı. 2000. Elimination of Essential (Cu, Zn) and Non-essential (Cd, Pb) Metals from Tissues of a Freshwater Fish Tilapia zilli. Turkish Journal of Zoology, 24: 429-436.
14. Khan, S., and D. Nugegoda. 2003. Australian Freshwater Crayfish Cherax destructor Accumulates and Depurates Nickel. Bulletin of Environmental Contamination and Toxicology, 70: 308–314.
15. Langston, W. J. 1990. Toxic Effects of Metals and the Incidence of Marine Ecosystems In: Heavy Metals in the Marine Environment (eds: Furness R.W., Rainbow P.S.). CRC Press, New York. 256.
16. Linde, A. R., Sanchez-Galan, S., Klein, D., Garcia-Vazquez, E., and K. H. Summer. 1999. Metalothionein and Heavy Metals in Brown Trout (Salmo trutta) and European Eel (Anguilla anguilla): A Comparative Study. Ecotoxicology and Environmental Safety, 44: 168-173.
17. Logan, M. 2010. Biostatistical Design and Analysis Using a Practical Guide. Wiley- Blackwell, London. 546.
18. Madigosky, S. R., Alvarez-Hernandez, X., and J. Glass. 1991. Lead, Cadmium, and Aluminum Accumulation in the Red Swamp Crayfish Procambarus clarkii Collected from Roadside Drainage Ditches in Louisiana. Archives of Environmental Contamination and Toxicology, 20(2): 253–8.
19. Meyer, W., Kretschmer, M., Hoffmann, A., and G. Harisch. 1991. Biochemical and Histochemical Observations on Effects of LowLevel Heavy Metal Load (Lead, Cadmium) in Different Organ Systems of The Freshwater Crayfish, Astacus astacus L. (Crustacea: Decapoda). Ecotoxicology and Environmental Safety, 21(2): 137–56.
20. Rainbow, P. S., and W.X. Wang. 2001. Comparative Assimilation of Cd, Cr, Se, and Zn by the Barnacle Elminius modestus from Phytoplankton and Zooplankton Diets. Marine Ecology Progress Series, 218: 239–48.
21. Rainbow, P. S., 2002. Trace Metal Concentrations in Aquatic Invertebrates: Why and so What? Environmental Pollution, 120: 497–507.
22. Reichert, W. L., Federighi, D.A., and D.C. Malins. 1979. Uptake and Metabolism of Lead and Cadmium in Coho salmon (Oncorhynchus kisutch). Comparative Biochemistry and Physiology, 63: 229–234.
23. Roesijadi, G., and W.E. Robinson. 1994. Metal Regulation in Aquatic Animals: Mechanism of Uptake, Accumulation and Release. In: Aquatic Toxicology; Molecular, Biochemical and Cellular Perspectives.(eds: Malins D.C., Ostrander G.K.) Lewis Publishers, London. 539.
24. Sivaperumal, P., Sankar, T. V., and P.G. Nair. 2007. Heavy Metal Concentration in Fish, Shell Fish and Fish Products From Internal Markets of Indiavis International Stand. Food Chemistry, 102: Selvi et al. Marine Science and Technology Bulletin (2012) 1:13-16 15 612–620.
25. Su, G. S., Martillano, K. J., Alcantara, T. P., Ragragio, E., De Jesus, J., Hallare, A., and G. Ramos. 2009. Assessing Heavy Metals in the Waters, Fish and Macroinvertebrates in Manila Bay, Philippines. Journal of Applied Sciences in Environmental Sanitation, 4(3):187-195.
26. Talbot, V., and A. Chegwidden. 1982. Cadmium and Other Heavy Metal Concentrations in Selected Biota from Cockburn Sound, Western Australia. Marine & Freshwater Research, 33(5): 779–788.
27. Torreblanca, A., Del Ramo, J., Arnau, J. A., and J. Diaz-Maynas. 1989. Cadmium, Mercury and Lead Effects on Gill Tissue of Freshwater Crayfish Procambarus clarkii (Girard). Biological Trace Element Research, 21: 343-347.
28. Tulasi, S. J., Reddy, P.U., and J. V. Ramana Rao. 1992. Accumulation of Lead and Effects on Total Lipid Derivatives in the Freshwater Fish Anabas testudineus (Bloch). Ecotoxicology and Environmental Safety, 23(1): 33-38.
29. Wicklund, A., Runn, P., and L. Norrgren. 1998. Cadmium and Zinc Interaction in Fish Effects of zinc on Uptake, Organ Distribution and Elimination of Cd in Zebre fish Brachydanio rerio. Archives of Environmental Contamination and Toxicology, 17: 245–354.