Investigation of Cryptosporidium sp. Oocysts in Erzurum’s Potable Water On Different Months

Volume: 2 Number: 3 December 26, 2016
Ahmet Yılmaz , Önder Akkaş , Hakan Uslu
EN

Investigation of Cryptosporidium sp. Oocysts in Erzurum’s Potable Water On Different Months

Abstract

Objective: Cryptosporidium sp. is a protozoan which is highly resistant to external environmental conditions and chlorination and can lead to severe diarrhea in immunosuppressed persons. Oocysts of this parasite, is excreted by human and animal feces, lead to contamination of potable (drinking) water supplies in environments with poor sanitation. Our aim is to investigate the presence of oocysts in some potable water samples taken from different points of Erzurum city center and around on different months.

Methods: Totally 120 water samples were collected from 40 random different points of Erzurum city center and around on April, May and July. Of the 120 samples, 45 were from city water system and 75 from fountain and wells water. Water samples collected within 5 liter tanks were filtered by using membrane filter. From each sample preparation were done by using modified acid fast staining method and then examined under microscope.

Results: Cryptosporidium sp. oocysts were detected on 18 (15.0%) of total 120 water samples. 6 (13.3%) of these positive samples were from city water system and the other 12 (16.0%) were from fountains and wells. According to the seasonal distribution of positive samples 9 (22.5%) have been taken on April, 7 (17.5%) on May and 2 (5.0%) on July.

Conclusion: Results of this study have shown that source of water supplies in our region are notably contaminated with Cryptosporidium sp. oocysts, and the rate of contamination is higher on April and May when compared with July.

Keywords

Cryptosporidium sp,water,modified acid fast

References

  1. Aksoy U, Akısu C, Sahin S, Usluca S, Yalcın G, Kuralay F et al. First reported waterborne outbreak of cryptosporidiosis with Cyclospora co-infection in Turkey. Euro Surveill 2007;12: E070215.
  2. Almeida A, Moreira MJ, Soares S, Delgado ML, Figueiredo J, Silva E et al. Presence of Cryptosporidium spp. and Giardia duodenalis in drinking water samples in the north of Portugal. Korean J Parasitol 2010; 48: 43-48.
  3. Ceber K, Aslan G, Otag F, Delialioğlu N, Ozturk C, Babur C et al. Investigation of Cryptosporidium spp. Oocysts in Tap Water, Well Water, Sewage Water and Sea Water in Mersin, Turkish Journal of Parasitology 2005; 29: 224-228.
  4. Chalmers RM, Katzer F. Looking for Cryptosporidium: the application of advances in detection and diagnosis. Trends Parasitol 2013; 29: 237-251.
  5. Cicek, M. Korkoca H, Akkas O. Investigation for Cryptosporidium spp. oocysts of drinking of water in Van. Turkish Bulletin of Hyg Experimental Biology 2011; 68 (3): 122-126.
  6. Clark DP. New insights into human cryptosporidiosis. Clin Microbiol Rev 1999; 12: 554–563.
  7. Conio O, Palumbo F, Borelli E, Carraro E, Pignata C. Cryptosporidium and Giardia oocysts occurrence in raw and drinking water in Italy. 20th Annual Meeting of the Italian Section Society of Protozoologists, Genova- İtaly: 1999. October 8–9.
  8. Current WL, Garcia LS. Cryptosporidiosis. Clin Microbiol Rev 1991; (4): 225-228.
  9. Eren C. To investigate the Cryptosporidium in immunosuppressed subjects of Different group by using modified acid-fast painting and ELISA methods, Diyarbakir: Medical Faculty of Dicle University. 2011.
  10. Dirim D, Turgay N, Alkan M Z. The observation of a Cryptosporidiosis cases with Kinyon acid-fast staining and polymerase chain reaction. Turkish Journal of Parasitology 2003; 27: 237-239.
Vancouver
1.Ahmet Yılmaz, Önder Akkaş, Hakan Uslu. Investigation of Cryptosporidium sp. Oocysts in Erzurum’s Potable Water On Different Months. Mid Blac Sea J Health Sci. 2016 Dec. 1;2(3):1-6. doi:10.19127/mbsjohs.282374