Antimicrobial Activity Of Silver Nanoparticles Synthesized with Extract of Tomato plant Against Bacterial and Fungal Pathogens

Year 2019, , 67 - 73, 28.08.2019

Mehmet Fırat Baran , Hilal Acay

https://doi.org/10.19127/mbsjohs.551132

Cited By: 13

Abstract

Objective:
Silver nanoparticles (AgNPs) have a wide range of
applications. Environmental-friendly synthesis methods for these nanoparticles
are more preferable due to their various advantages. This study aimed to
synthesize AgNPs using the extract of the tomato plant in an easy and
economical way. and testing this AgNPs against some human pathogens.

Methods:
Silver nanoparticles were synthesized using aqueous
silver nitrate and reducing tomato plant extract. The characterization of AgNPs
was determined by ultraviolet-visible spectrophotometry (UV-Vis), X-ray
crystallography (XRD) Scanning electron microscopy (SEM), Fourier transform
infrared Spectroscopy (FT-IR), energy dispersive X-ray spectrum (EDAX),
thermogravimetric - differential thermal analysis (TGA-DTA) data. The effects
of the particles on pathogenic microorganisms were determined by minimum
inhibition concentration (MIC).

Results:
These data, with a maximum absorbance of 450.51 nm, in
the spherical view, with the peaks and values of 111^o, 200^o,
220^o and 311^o (38.08, 44.28, 64.42 and 77.34), AgNPs
showed a cubic crystal structure and, using the Debye-Scherrer equation, it was
determined that they had a crystal size of 21.11 nm AgNPs had an antimicrobial
activity on hospital pathogens gram negative, gram positive and Candida
albicans yeast.

Conclusion:
We found that these particles showed antimicrobial
activity on various microorganisms even at very high concentrations. As a
solution to the antimicrobial search, it can be developed in medical industry.

Keywords

Antimicrobial activity, XRD, SEM, TGA-DTA, Silver nanoparticle

References

• Ahmed B, Hashmi A, Khan MS, Musarrat J. ROS mediated destruction of cell membrane, growth and biofilms of human bacterial pathogens by stable metallic AgNPs functionalized from bell pepper extract and quercetin, Adv Powder Technol, 2018, 29(7): 1601–1616. 03.025
• Ali K, Ahmed B, Dwivedi S, Saquib Q, Al-Khedhairy A A, Musarrat J. Microwave accelerated green synthesis of stable silver nanoparticles with Eucalyptus globulus leaf extract and their antibacterial and antibiofilm activity on clinical isolates, PLoS One, 2015,10(7): 1–20
• Alsammarraie FK, Wang W, Zhou P, Mustapha A, Lin M. Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities”, Colloids Surfaces B Biointerfaces, 2018, 171: 398–405..
• Baran MF: Synthesis, Characterization And Investigation Of Antimicrobial Activity Of Silver Nanoparticles From Cydonia Oblonga Leaf, 2019, 17(2): 2583–2592.
• Begum N A, Mondal S, Basu S, Laskar R A, Mandal D. Biogenic synthesis of Au and Ag nanoparticles using aqueous solutions of Black Tea leaf extracts, Colloids Surfaces B Biointerfaces, 2009, 71 (1): 113–118.
• Beyene HD, Werkneh AA, Bezabh H K, AAmbaye T G: Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review, Sustain Mater Technol, 2017, 13, January,; 18–23,
• Brandt O, Mildner M, Egger AE, et al. Nanoscalic silver possesses broad-spectrum antimicrobial activities and exhibits fewer toxicological side effects than silver sulfadiazine”, Nanomedicine Nanotechnology, Biol Med, 2012, 8(4) :478–488. .
• Ferreyra Maillard A P V, Dalmasso P R, López de Mishima B A, Hollmann A. Interaction of green silver nanoparticles with model membranes: possible role in the antibacterial activity, Colloids Surfaces B Biointerfaces, 2018, 171: 320–326.
• Gliga AR, Skoglund S, Wallinder IO, Fadeel B, Karlsson HL. Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release, Part Fibre Toxicol, 2014, 11(1): 11.
• Gopinath K, Kumaraguru S, Bhakyaraj K, Mohan S, Venkatesh KS, Esakkirajan M, et al. Green synthesis of silver, gold and silver/gold bimetallic nanoparticles using the Gloriosa superba leaf extract and their antibacterial and antibiofilm activities”, Microb Pathog, 2016, (101): 1–11.
• Hemmati S, Rashtiani A, Zangeneh MM, Mohammadi P, Zangeneh A, Veisi H. Green synthesis and characterization of silver nanoparticles using Fritillaria flower extract and their antibacterial activity against some human pathogens, Polyhedron, 2019, 158: 8–14,
• Jain D , Daima HK. , Kachhwaha S., Kothari SL., Synthesis of plant-mediated silver nanoparticles using papaya fruit extract and evaluation of their antimicrobial activities Digest Journal of Nanomaterials and Biostructures, 2009,( 4): 557-563.
• Khan AU, Yuan Q, Khan ZUH, Ahmad A, Khan FU, Tahir K, Shakeel M, Ullah S. An eco-benign synthesis of AgNPs using aqueous extract of Longan fruit peel: Antiproliferative response against human breast cancer cell line MCF-7, antioxidant and photocatalytic deprivation of methylene blue, J Photochem Photobiol B Biol, 2018.
• Kobashigawa JM, Robles CA, Martínez Ricci ML, Carmarán CC: Influence of strong bases on the synthesis of silver nanoparticles (AgNPs) using the ligninolytic fungi Trametes trogii, Saudi J Biol Sci, 2018, 4–10.
• Kumar V, Gundampati R K, Singh D K, Bano D, Jagannadham M V, Hasan S H. Photoinduced green synthesis of silver nanoparticles with highly effective antibacterial and hydrogen peroxide sensing properties, J Photochem Photobiol B Biol, 2016,(162):374–385.
• Lloyd JR, Yong P, Macaskie LE. Enzymatic recovery of elemental palladium by using sulfate-reducing bacteria, Appl Environ Microbiol, 1998, 64 (11): 4607–4609.
• Nanda A, Nayak BK, Krishnamoorthy M. Antimicrobial properties of biogenic silver nanoparticles synthesized from phylloplane fungus, Aspergillus tamarii, Biocatal Agric Biotechnol, 16 August 2018, 225–228.
• Nishanthi R. Malathi S. John Paul S. Palani P. Green synthesis and characterization of bioinspired silver, gold and platinum nanoparticles and evaluation of their synergistic antibacterial activity after combining with different classes of antibiotics, Mater Sci Eng C, 2019, 96, 693–707.
• Prakash P, Gnanaprakasam P, Emmanuel R, Arokiyaraj S, Saravanan M. Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates, Colloids Surfaces B Biointerfaces, 2013, 108, 255–259.
• Prakash S, Elavarasan N, Venkatesan A, Subashini K, Sowndharya M, Sujatha V. Green synthesis of copper oxide nanoparticles and its effective applications in Biginelli reaction, BTB photodegradation and antibacterial activity, Adv Powder Technol, 2018.
• Pugazhendhi S, Palanisamy PK, Jayavel R. Synthesis of highly stable silver nanoparticles through a novel green method using Mirabillis jalapa for antibacterial, nonlinear optical applications, Opt Mater (Amst), 2018, 79, 457–463.
• Sagar G, Ashok B. Green Synthesis of Silver Nanoparticles Using Aspergillus niger and Its Efficacy Against Human Pathogens, Eur J Exp Biol, 2012, 2 (5): 1654–1658. Saha J, Begum A, Mukherjee A, Kumar S. A novel green synthesis of silver nanoparticles and their catalytic action in reduction of Methylene Blue dye, Sustain Environ Res, 2017, 27(5): 245–250.
• Sarkar MK, Vadivel V, Charan Raja MR, Mahapatra SK. Potential anti-proliferative activity of AgNPs synthesized using M. longifolia in 4T1 cell line through ROS generation and cell membrane damage, J Photochem Photobiol B Biol, 2018.
• Sengottaiyan A, Mythili R, Selvankumar T, Aravinthan A, Kamala-Kannan S, Manoharan K, Thiyagarajan P, Govarthanan M, Jong-Hoon Kim. Green synthesis of silver nanoparticles using Solanum indicum L. and their antibacterial, splenocyte cytotoxic potentials, Res Chem Intermed, 2016, 42 (4): 3095–3103.
• Shao Y, Wu C, Wu T, Yuan C, Chen S, Ding T, Ye X, Hu Y. Green synthesis of sodium alginate-silver nanoparticles and their antibacterial activity, Int J Biol Macromol, 2018.
• Singh A, Sharma B, Deswal R. Green silver nanoparticles from novel Brassicaceae cultivars with enhanced antimicrobial potential than earlier reported Brassicaceae members, J Trace Elem Med Biol, 2018, 47, , 1–11.
• Sinsinwar S, Sarkar MK, Suriya KR, Nithyanand P, Vadivel V. Use of agricultural waste (coconut shell) for the synthesis of silver nanoparticles and evaluation of their antibacterial activity against selected human pathogens, Microb Pathog, 2018, 124,30–37
• Song JY, Kwon EY, Kim BS. Biological synthesis of platinum nanoparticles using Diopyros kaki leaf extract, Bioprocess Biosyst Eng, 2010, 33(1):159–164.
• Tovar-Corona A, Lobo-Sánchez MA, Herrera-Perez J L, Zanella R, Rodriguez-Mora J I, Vázquez-Cuchillo O. Green synthesis of copper (0) nanoparticles with cyanidine-O-3-glucoside and its strong antimicrobial activity, Mater Lett, 2018, 211, 266–269.
• Vetchinkina EP, Loshchinina EA, Vodolazov IR, Kursky VF, Dykman LA, Nikitina VE. Biosynthesis of nanoparticles of metals and metalloids by basidiomycetes. Preparation of gold nanoparticles by using purified fungal phenol oxidases, Appl. Microbiol Biotechnol, 2016, 1–16.