Antimicrobial Activity Of Silver Nanoparticles Synthesized with Extract of Tomato plant Against Bacterial and Fungal Pathogens
Abstract
Objective: Silver nanoparticles (AgNPs) have a wide range of applications. Environmental-friendly synthesis methods for these nanoparticles are more preferable due to their various advantages. This study aimed to synthesize AgNPs using the extract of the tomato plant in an easy and economical way. and testing this AgNPs against some human pathogens.
Methods: Silver nanoparticles were synthesized using aqueous silver nitrate and reducing tomato plant extract. The characterization of AgNPs was determined by ultraviolet-visible spectrophotometry (UV-Vis), X-ray crystallography (XRD) Scanning electron microscopy (SEM), Fourier transform infrared Spectroscopy (FT-IR), energy dispersive X-ray spectrum (EDAX), thermogravimetric - differential thermal analysis (TGA-DTA) data. The effects of the particles on pathogenic microorganisms were determined by minimum inhibition concentration (MIC).
Results: These data, with a maximum absorbance of 450.51 nm, in the spherical view, with the peaks and values of 111o, 200o, 220o and 311o (38.08, 44.28, 64.42 and 77.34), AgNPs showed a cubic crystal structure and, using the Debye-Scherrer equation, it was determined that they had a crystal size of 21.11 nm AgNPs had an antimicrobial activity on hospital pathogens gram negative, gram positive and Candida albicans yeast.
Conclusion: We found that these particles showed antimicrobial activity on various microorganisms even at very high concentrations. As a solution to the antimicrobial search, it can be developed in medical industry.
Keywords
References
- Ahmed B, Hashmi A, Khan MS, Musarrat J. ROS mediated destruction of cell membrane, growth and biofilms of human bacterial pathogens by stable metallic AgNPs functionalized from bell pepper extract and quercetin, Adv Powder Technol, 2018, 29(7): 1601–1616. 03.025
- Ali K, Ahmed B, Dwivedi S, Saquib Q, Al-Khedhairy A A, Musarrat J. Microwave accelerated green synthesis of stable silver nanoparticles with Eucalyptus globulus leaf extract and their antibacterial and antibiofilm activity on clinical isolates, PLoS One, 2015,10(7): 1–20
- Alsammarraie FK, Wang W, Zhou P, Mustapha A, Lin M. Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities”, Colloids Surfaces B Biointerfaces, 2018, 171: 398–405..
- Baran MF: Synthesis, Characterization And Investigation Of Antimicrobial Activity Of Silver Nanoparticles From Cydonia Oblonga Leaf, 2019, 17(2): 2583–2592.
- Begum N A, Mondal S, Basu S, Laskar R A, Mandal D. Biogenic synthesis of Au and Ag nanoparticles using aqueous solutions of Black Tea leaf extracts, Colloids Surfaces B Biointerfaces, 2009, 71 (1): 113–118.
- Beyene HD, Werkneh AA, Bezabh H K, AAmbaye T G: Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review, Sustain Mater Technol, 2017, 13, January,; 18–23,
- Brandt O, Mildner M, Egger AE, et al. Nanoscalic silver possesses broad-spectrum antimicrobial activities and exhibits fewer toxicological side effects than silver sulfadiazine”, Nanomedicine Nanotechnology, Biol Med, 2012, 8(4) :478–488. .
- Ferreyra Maillard A P V, Dalmasso P R, López de Mishima B A, Hollmann A. Interaction of green silver nanoparticles with model membranes: possible role in the antibacterial activity, Colloids Surfaces B Biointerfaces, 2018, 171: 320–326.
- Gliga AR, Skoglund S, Wallinder IO, Fadeel B, Karlsson HL. Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release, Part Fibre Toxicol, 2014, 11(1): 11.
- Gopinath K, Kumaraguru S, Bhakyaraj K, Mohan S, Venkatesh KS, Esakkirajan M, et al. Green synthesis of silver, gold and silver/gold bimetallic nanoparticles using the Gloriosa superba leaf extract and their antibacterial and antibiofilm activities”, Microb Pathog, 2016, (101): 1–11.