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LARİNKS PRİMER SKUAMÖZ HÜCRELİ KARSİNOMLARINDA HUMAN PAPİLLOMA VİRÜS SIKLIĞI, p53 ve Ki-67 EKSPRESYONLARININ KLİNİKOPATOLOJİK KORELASYONU

Yıl 2013, Cilt: 14 Sayı: 3, 13 - 20, 01.12.2013

Öz

AMAÇ: Larinks kanseri gelişiminde son yıllarda Human Papillomavirüs (HPV) enfeksiyonları üzerinde durulmaktadır. Bu çalışmada larinks Skuamöz hücreli karsinom (SHK)'larında HPV varlığının immünohistokimyasal ve Polimeraz zincir reaksiyonu (PZR) dizisi hibrizasyon yöntemiyle araştırılması, ayrıca p53, Ki-67 ekspresyonunun HPV varlığı ve klinikopatolojik parametrelerle ilişkisinin ortaya konması amaçlanmıştır.GEREÇ ve YÖNTEM: Larinks SHK nedeniyle opere edilen 47 olgu retrospektif olarak çalışmaya alındı. Parafinli bloklardan hazırlanan kesitlere Avidin-Biyotin kompleks sistemiyle panHPV, HPV-16, HPV-18, p53 ve Ki-67 immünohistokimyasal boyaları uygulandı. Parafin bloklardan hazırlanan kesitlerden DNA izolasyonu yapıldı. Sonrasında HPV varlığı HybriBio HPV Genoarray Test ile araştırıldı.BULGULAR: PZR-array hibridizasyon yöntemi ile olguların 15'inde (% 31,9) poliklonal HPV pozitifliği saptanmıştır. İki olguda literatürden farklı olarak HPV 68 bulunmuştur. HPV (PZR) pozitif bulunan olgularda anlamlı düzeyde yüksek Ki-67 boyanması (p=0,01) saptanmıştır. Tümörlerde diferansiyasyon azaldıkça p53 (p=0,041) ekspresyonunun arttığı görülmüştür. Ki-67 ile panHPV (p=0,004) ve HPV-16 (p=0,002) boyanması karşılaştırıldığında, pozitif olgularda Ki-67 ekspresyonunun yüksek olduğu gözlenmiştir.SONUÇ: Formalin fiske parafine gömülü dokular HPV varlığının gösterilmesinde kullanılabilir. HybriBio HPV Genoarray test metodu diğer metodlara göre kullanımı oldukça basittir ve HPV-DNA saptanmasında kıymetli bir araç olabilir. Literatür araştırıldığında şimdiye kadar HPV 68 virüsünün larinks SHK'larında saptanmadığı görülmüştür. Ki-67 ve p53 ekspresyonları larinks SHK'larında iyi veya kötü prognoz açısından yol gösterici olabilir

Kaynakça

  • 1. Lazaris AC, Lendari I, Kavantzas N, Kandiloros D, Adamopoulos G, Davaris P. Correlation of tumor markers p53, bcl-2 and cathepsin-D with clinicopathologic features and disease-free survival in laryngeal squamous cell carcinoma. Pathol Int 2000;50(9):717-24.
  • 2. Robin PE, Reid A, Powell DJ, McConkey CC. The incidence of cancer of the larynx. Clin Otolaryngol Allied Sci 1991;16(2):198-201.
  • 3. Morshed K. Difference between human papillomavirus infection and laryngeal squamous cell carcinoma. J Med Virol 82(6):1017-23.
  • 4. Du J, Lu X, Liang J, YangY, Lin J, Zhu X, Xu j. Detection and typing of human papillomavirus (HPV) in Condyloma acuminatumand bowenoid papulosis HybriBio HPV GenoArray testkit, real time polymerase chain reaction (PCR) and sequencing. Afr J Pharm Pharmacol 2013;7(3):73-7.
  • 5. Öktem F, Aydın S, Öz F, Toprak M, Ada M. Baş-boyun kanserlerinde Human papilloma virus infeksiyonunun rolü. K.B.B. ve Baş Boyun Cerrahisi Dergisi 2000;8 (1): 51-7.
  • 6. Brandwein MS, Nuovo GJ, Biller H. Analysis of prevalence of human papillomavirus in laryngeal carcinomas. Study of 40 cases using polymerase chain reaction and consensus primers. Ann Otol Rhinol Laryngol 1993;102(4 pt 1):309-13.
  • 7. Kong CS, Welton ML, Longacre TA. Role of human papillomavirus in squamous cell metaplasia-dysplasiacarcinoma of the rectum. Am J Surg Pathol 2007;31(6):919-25.
  • 8. Mills A, Balasubramaniam R, Longacre TA, Kong CS, Pinsky BA. Laboratory-developed L1 sequencing and type-spesific, real time polymerase chain reaction for the detection and typing of human papillomaviruses in formalin-fixed, paraffin-embedded tissues. Arch Pathol Lab Med 2013;137(19):50-4.
  • 9. Nakashima T, Wang XF, Masuda M, Inokuchi A, Komiyama S. Overexpression of p53 nuclear protein in premalignant and malignant laryngeal lesions. Eur Arch Otorhinolaryngol 1999;256(1):56-9.
  • 10. Rodriguez Tojo MJ, Garcia Cano FJ, Infante Sanchez JC, Velaquez fernandez E, Aquirre Urizar JM. Immunoexpression of p53, Ki-67 and E-cadherin in basaloid squamous cell carcinoma of the larynx. Clin Transl Oncol 2005;7 (3):110-4.
  • 11. Calli C, Calli A, Pinar E, Oncel S, Demirtaşoğlu F. Expression of Ki-67 and p53 in laryngeal squamous cell carcinomas. Kulak Burun Bogaz Ihtis Derg 2005; 15(1-2):9-13.
  • 12. Rosai J. Larynx. In: Rosai J, editor. Rosai and Ackerman's surgical pathology. 9th ed. Mosby Inc, New York,2004:335-48.
  • 13. Acikalin MF, Oner U, Tel N, Paşaoğlu O, Cakli H, Colak E. Prognostic significance of Ki-67 expression for patients with laryngeal squamous cell carcinoma primarily treated by total laryngectomy. Eur Arch Otorhinolaryngol 2004;261(7):376-80.
  • 14. Grisaru D, Avidor B, Niv J, Marmor S, Almog B, Leibowitz C, Graidy M, Giladi M. Pilot study of prevalence of high-risk human papillomavirus genotypes in Israeli Jewish women referred for colposcopic examination. J Clin Microbiol 2008;46(5):1602-5.
  • 15. Ergünay K, Mısırlıoglu M, Fırat P, Tuncer Z.S, Tuncer S, Yıldız I, Ustaçelebi S. Detection and typing of human papilloma virus by polymerase chain reaction and hybridization assay in cervical samples with cytological abnormalities. Mikrobiyol Bül 2008;42(2):273-82.
  • 16. De Oliveira DE, Bacchi MM, Macarenco RS, Taqliarini JV, Cordeir RC, Bacchi CE. Human papillomavirus and Epstein-Barr virus infection, p53 expression, and cellular proliferation in laryngeal carcinoma. Am J Clin Pathol 2006;126(2):284-93.
  • 17. Vielba R, Bilbao J, Ispizua A, Zabalza I, Alfaro J, Rezola R, Moreno E, Elloriaga J, Alonso I, Baroja A, de la Hoz C. p53 and Cyclin D1 as prognostic factors in squamous cell carcinoma of the larynx. Laryngoscope 2003;113(1):167-72.
  • 18. Sas-Korczynska B, Korzeniowski S, Skolyszewski J. Cancer of the larynx in females. Cancer Radiother 2003;7(6):380-5.
  • 19. Leon X, Rinaldo A, Saffiotti U, Ferlito A. Laryngeal cancer in non-smoking and nondrinking patients. Acta Otolaryngol 2004; 124 (6): 664-669
  • 20. Kaufman JA, Burke AJ. The etiology and pathogenesis of laryngeal carcinoma. Otolaryngol Clin North Am 1997;30(1):1-19.
  • 21. Ribeiro-Silva A, Zambelli Ramalho LN, Britto Garcia S, Zucoloto S. The relationship between p63 and p53 expression in normal and neoplastic breast tissue. Arch Pathol Lab Med 2003;127(3):336-40.
  • 22. Rabah R, Sakr W, Thomas R, Lancaster WD, Gregoire L. Human papillomavirustype, proliferative activity, and p53: potential markers of aggressive papillomatosis. Arch Pathol Lab Med 2000;124(5): 721-4.
  • 23. Hofseth LJ, Hussain SP, Harris CC. p53: 25 years after its discovery. Trends Pharmacol Sci 2004;25(4):177- 81.
  • 24. Kazkayasi M, Hücümenoğlu S, Siriner Gİ, Hücümenoğlu M. Over-expression of p53 and c-erbB2 oncoproteins in laryngeal carcinoma. Eur Arch Otorhinolaryngol 2001;258(7):329-35.
  • 25. Jacob SE, Sreevidya S, Chacko E, Pillai MR. Cellular manifestations of human papillomavirus infection in laryngeal tissues. J Surg Oncol 2002; 79 (3): 142-150.
  • 26. Hirai T, Hayashi K, Takumida M, Ueda T, Hirakawa K, Yajin K. Reduced expression of p27 is correlated with progression in precancerous lesions of the larynx. Auris Nasus Larynx 2003;30(2):163-8.
  • 27. Liu M, Lawson G, Delos M, Jamart J, Chatelain B, Remacle M, Marbaix E. Prognostic value of cell proliferation markers, tumour suppressor proteins and cell adhesion molecules in primary squamous cell carcinoma of the larynx and hypopharynx. Eur Arch Otorhinolaryngol 2003;260(1):28-34.
  • 28. Lera J, Lara PC, Perez S, Cabrera JL, Santana C. Tumor proliferation, p53 expression, and apoptosis in laryngeal carcinoma: relation to the results of radiotherapy. Cancer 1998;83(12):2493-2501.
  • 29. Ullman EV. On the aetiology of the laryngeal papilloma. Acta Oto-laryngol 1923; 5: 317–334.
  • 30. Adelstein DJ, Ridge JA, Gillison ML, Chaturvedi AK, D'Souza G, Gravitt PE, Westra W, Psyrri A, Kast WM, Koutsky LAet al. Head and neck squamous cell cancer and the human papillomavirus: summary of a National Cancer Institute State of the Science Meeting, November 9–10, 2008, Washington, D.C. Head Neck 2009;31(11):1393-422.
  • 31. Fakhry C, Westra WH, Li S, Cmelak A, Ridge JA, Pinto H, Forastiere A, Gillison ML. Improved survival of patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial. J Natl Cancer Inst 2008;100(4):261-9.
  • 32. Dönmez M, Erdem Huq E, Akdur NC, Gözel S, Bozkurt RE. p16 expression in laryngeal squamous cell carcinoma and its relationship with human papillomavırus. Turkiye Klinikleri J Med Sci 2012;32(6):1576-85.
  • 33. Masanori H, Hajime S, Koma M. Loop-mediated isothermal amplification method for detection of human papilomavirus type 6, 11, 16 and 18. J Med Virol 2007;79(5):605-15.
  • 34. Poljak M, Kocjan BJ. Commercially available assays for multiplex detection of alpha human papillomaviruses. Expert Rev Anti Infect Ther 2010;8(10):1139-62.
  • 35. Morshed K, Polz-Dacewicz M, Szymański M, Smoleń A. Usefulness and efficiency of formalin-fixed paraffin-embedded specimens from laryngeal squamous cell carcinoma in HPV detection by IHC and PCR/ D E I A . F o l i a H ist o c h e m Cy t o b i o l 2010;48(3):398-402.
  • 36. Gorgoulis VG, Zacharatos P, Kotsinas A, Kyroudi A, Rassidakis AN, Ikonomopoulos JA, Barbatis C, Herrington CS, Kittas C. Human papilloma virus (HPV) is possibly involved in laryngeal but not in lung carcinogenesis. Hum Pathol 1999;30(3):274-83.

Frequency of Human Papillomavirus in Larynx Squamous Cell Carcinoma, Clinicopathological Correlation of p53 and Ki-67 Expressions

Yıl 2013, Cilt: 14 Sayı: 3, 13 - 20, 01.12.2013

Öz

OBJECTIVE: Human Papillomavirus (HPV) infections have been discussed with regard to laryngeal carcinogenesis recent years. This study investigated the presence of HPV in larynx squamous cell carcinomas (SCC) via immunohistochemical and Polymerase chain reaction (PCR) array hybridization method and the relationship between p53, Ki-67 expression with HPV presence and clinicopathologic parameters. MATERIAL and METHODS: 47 patients who had been operated for larynx SCC were retrospectively included in the study. PanHPV, HPV-16, HPV-18, p53 and Ki-67 immunohistochemical stains were applied to crosssections obtained from waxed blocks by means of Avidin-Biyotin complex system. DNA was isolated from the cross-sections. The presence of HPV was investigated via a HybriBio HPV GenoArray diagnostic kit. RESULTS: Polyclonal HPV positivity was detected only in 15 (31.9%) cases by means of PCR-array hybridization method. Contrary to the literature, HPV-68 was found in two cases. In HPV (PCR)-positive cases, Ki-67 staining was found at high grade (p=0.01). The expression of p53 (p=0.041) increases as differentiation decreases in tumors. When comparing Ki-67, panHPV (p=0.004) and HPV-16 (p=0.002) staining, the expression of Ki-67 is higher than others in positive cases. CONCLUSION: Formalin-fixed paraffin-embedded tissues can be used to indicate the presence of HPV. The use of the HybriBio HPV GenoArray test was much more straightforward than other methods and is useful in detecting HPV-DNA. In the literature, no HPV-68 virus was detected in larynx SCCs until recently. The expression of Ki-67 and p53 could be central to prognosis in larynx SCCs.

Kaynakça

  • 1. Lazaris AC, Lendari I, Kavantzas N, Kandiloros D, Adamopoulos G, Davaris P. Correlation of tumor markers p53, bcl-2 and cathepsin-D with clinicopathologic features and disease-free survival in laryngeal squamous cell carcinoma. Pathol Int 2000;50(9):717-24.
  • 2. Robin PE, Reid A, Powell DJ, McConkey CC. The incidence of cancer of the larynx. Clin Otolaryngol Allied Sci 1991;16(2):198-201.
  • 3. Morshed K. Difference between human papillomavirus infection and laryngeal squamous cell carcinoma. J Med Virol 82(6):1017-23.
  • 4. Du J, Lu X, Liang J, YangY, Lin J, Zhu X, Xu j. Detection and typing of human papillomavirus (HPV) in Condyloma acuminatumand bowenoid papulosis HybriBio HPV GenoArray testkit, real time polymerase chain reaction (PCR) and sequencing. Afr J Pharm Pharmacol 2013;7(3):73-7.
  • 5. Öktem F, Aydın S, Öz F, Toprak M, Ada M. Baş-boyun kanserlerinde Human papilloma virus infeksiyonunun rolü. K.B.B. ve Baş Boyun Cerrahisi Dergisi 2000;8 (1): 51-7.
  • 6. Brandwein MS, Nuovo GJ, Biller H. Analysis of prevalence of human papillomavirus in laryngeal carcinomas. Study of 40 cases using polymerase chain reaction and consensus primers. Ann Otol Rhinol Laryngol 1993;102(4 pt 1):309-13.
  • 7. Kong CS, Welton ML, Longacre TA. Role of human papillomavirus in squamous cell metaplasia-dysplasiacarcinoma of the rectum. Am J Surg Pathol 2007;31(6):919-25.
  • 8. Mills A, Balasubramaniam R, Longacre TA, Kong CS, Pinsky BA. Laboratory-developed L1 sequencing and type-spesific, real time polymerase chain reaction for the detection and typing of human papillomaviruses in formalin-fixed, paraffin-embedded tissues. Arch Pathol Lab Med 2013;137(19):50-4.
  • 9. Nakashima T, Wang XF, Masuda M, Inokuchi A, Komiyama S. Overexpression of p53 nuclear protein in premalignant and malignant laryngeal lesions. Eur Arch Otorhinolaryngol 1999;256(1):56-9.
  • 10. Rodriguez Tojo MJ, Garcia Cano FJ, Infante Sanchez JC, Velaquez fernandez E, Aquirre Urizar JM. Immunoexpression of p53, Ki-67 and E-cadherin in basaloid squamous cell carcinoma of the larynx. Clin Transl Oncol 2005;7 (3):110-4.
  • 11. Calli C, Calli A, Pinar E, Oncel S, Demirtaşoğlu F. Expression of Ki-67 and p53 in laryngeal squamous cell carcinomas. Kulak Burun Bogaz Ihtis Derg 2005; 15(1-2):9-13.
  • 12. Rosai J. Larynx. In: Rosai J, editor. Rosai and Ackerman's surgical pathology. 9th ed. Mosby Inc, New York,2004:335-48.
  • 13. Acikalin MF, Oner U, Tel N, Paşaoğlu O, Cakli H, Colak E. Prognostic significance of Ki-67 expression for patients with laryngeal squamous cell carcinoma primarily treated by total laryngectomy. Eur Arch Otorhinolaryngol 2004;261(7):376-80.
  • 14. Grisaru D, Avidor B, Niv J, Marmor S, Almog B, Leibowitz C, Graidy M, Giladi M. Pilot study of prevalence of high-risk human papillomavirus genotypes in Israeli Jewish women referred for colposcopic examination. J Clin Microbiol 2008;46(5):1602-5.
  • 15. Ergünay K, Mısırlıoglu M, Fırat P, Tuncer Z.S, Tuncer S, Yıldız I, Ustaçelebi S. Detection and typing of human papilloma virus by polymerase chain reaction and hybridization assay in cervical samples with cytological abnormalities. Mikrobiyol Bül 2008;42(2):273-82.
  • 16. De Oliveira DE, Bacchi MM, Macarenco RS, Taqliarini JV, Cordeir RC, Bacchi CE. Human papillomavirus and Epstein-Barr virus infection, p53 expression, and cellular proliferation in laryngeal carcinoma. Am J Clin Pathol 2006;126(2):284-93.
  • 17. Vielba R, Bilbao J, Ispizua A, Zabalza I, Alfaro J, Rezola R, Moreno E, Elloriaga J, Alonso I, Baroja A, de la Hoz C. p53 and Cyclin D1 as prognostic factors in squamous cell carcinoma of the larynx. Laryngoscope 2003;113(1):167-72.
  • 18. Sas-Korczynska B, Korzeniowski S, Skolyszewski J. Cancer of the larynx in females. Cancer Radiother 2003;7(6):380-5.
  • 19. Leon X, Rinaldo A, Saffiotti U, Ferlito A. Laryngeal cancer in non-smoking and nondrinking patients. Acta Otolaryngol 2004; 124 (6): 664-669
  • 20. Kaufman JA, Burke AJ. The etiology and pathogenesis of laryngeal carcinoma. Otolaryngol Clin North Am 1997;30(1):1-19.
  • 21. Ribeiro-Silva A, Zambelli Ramalho LN, Britto Garcia S, Zucoloto S. The relationship between p63 and p53 expression in normal and neoplastic breast tissue. Arch Pathol Lab Med 2003;127(3):336-40.
  • 22. Rabah R, Sakr W, Thomas R, Lancaster WD, Gregoire L. Human papillomavirustype, proliferative activity, and p53: potential markers of aggressive papillomatosis. Arch Pathol Lab Med 2000;124(5): 721-4.
  • 23. Hofseth LJ, Hussain SP, Harris CC. p53: 25 years after its discovery. Trends Pharmacol Sci 2004;25(4):177- 81.
  • 24. Kazkayasi M, Hücümenoğlu S, Siriner Gİ, Hücümenoğlu M. Over-expression of p53 and c-erbB2 oncoproteins in laryngeal carcinoma. Eur Arch Otorhinolaryngol 2001;258(7):329-35.
  • 25. Jacob SE, Sreevidya S, Chacko E, Pillai MR. Cellular manifestations of human papillomavirus infection in laryngeal tissues. J Surg Oncol 2002; 79 (3): 142-150.
  • 26. Hirai T, Hayashi K, Takumida M, Ueda T, Hirakawa K, Yajin K. Reduced expression of p27 is correlated with progression in precancerous lesions of the larynx. Auris Nasus Larynx 2003;30(2):163-8.
  • 27. Liu M, Lawson G, Delos M, Jamart J, Chatelain B, Remacle M, Marbaix E. Prognostic value of cell proliferation markers, tumour suppressor proteins and cell adhesion molecules in primary squamous cell carcinoma of the larynx and hypopharynx. Eur Arch Otorhinolaryngol 2003;260(1):28-34.
  • 28. Lera J, Lara PC, Perez S, Cabrera JL, Santana C. Tumor proliferation, p53 expression, and apoptosis in laryngeal carcinoma: relation to the results of radiotherapy. Cancer 1998;83(12):2493-2501.
  • 29. Ullman EV. On the aetiology of the laryngeal papilloma. Acta Oto-laryngol 1923; 5: 317–334.
  • 30. Adelstein DJ, Ridge JA, Gillison ML, Chaturvedi AK, D'Souza G, Gravitt PE, Westra W, Psyrri A, Kast WM, Koutsky LAet al. Head and neck squamous cell cancer and the human papillomavirus: summary of a National Cancer Institute State of the Science Meeting, November 9–10, 2008, Washington, D.C. Head Neck 2009;31(11):1393-422.
  • 31. Fakhry C, Westra WH, Li S, Cmelak A, Ridge JA, Pinto H, Forastiere A, Gillison ML. Improved survival of patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial. J Natl Cancer Inst 2008;100(4):261-9.
  • 32. Dönmez M, Erdem Huq E, Akdur NC, Gözel S, Bozkurt RE. p16 expression in laryngeal squamous cell carcinoma and its relationship with human papillomavırus. Turkiye Klinikleri J Med Sci 2012;32(6):1576-85.
  • 33. Masanori H, Hajime S, Koma M. Loop-mediated isothermal amplification method for detection of human papilomavirus type 6, 11, 16 and 18. J Med Virol 2007;79(5):605-15.
  • 34. Poljak M, Kocjan BJ. Commercially available assays for multiplex detection of alpha human papillomaviruses. Expert Rev Anti Infect Ther 2010;8(10):1139-62.
  • 35. Morshed K, Polz-Dacewicz M, Szymański M, Smoleń A. Usefulness and efficiency of formalin-fixed paraffin-embedded specimens from laryngeal squamous cell carcinoma in HPV detection by IHC and PCR/ D E I A . F o l i a H ist o c h e m Cy t o b i o l 2010;48(3):398-402.
  • 36. Gorgoulis VG, Zacharatos P, Kotsinas A, Kyroudi A, Rassidakis AN, Ikonomopoulos JA, Barbatis C, Herrington CS, Kittas C. Human papilloma virus (HPV) is possibly involved in laryngeal but not in lung carcinogenesis. Hum Pathol 1999;30(3):274-83.
Toplam 36 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA62TN85AR
Bölüm Araştırma Makalesi
Yazarlar

Mevlüt Çetin Bu kişi benim

Nesibe Kahraman Çetin Bu kişi benim

Sevin Kırdar Bu kişi benim

M. Gökhan Erpek Bu kişi benim

İbrahim Meteoğlu Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 14 Sayı: 3

Kaynak Göster

EndNote Çetin M, Kahraman Çetin N, Kırdar S, Erpek MG, Meteoğlu İ (01 Aralık 2013) Frequency of Human Papillomavirus in Larynx Squamous Cell Carcinoma, Clinicopathological Correlation of p53 and Ki-67 Expressions. Meandros Medical And Dental Journal 14 3 13–20.