A Novel Biomarker in Experimental Cerebral Ischemia: Junctional Adhesion Molecule-A

Yıl 2024, Cilt: 25 Sayı: 2, 85 - 96

Gül Taşlı Yeşilçayır , Yunus Emre Özlüer , Ozge Cevik , Erkan Gümüş

https://doi.org/10.69601/meandrosmdj.1522105

Öz

Objectives: To investigate the role of blood brain barrier biomarkers for the detection of experimental cerebral ischemia in rats.

Methods: Forty adult male Wistar albino rats with a mean age of 4–6 months and an average weight of 350–400 g were used in the study. The rats were divided into five ischemia groups (control, 1.5 h of ischemia, 4.5 h of ischemia, 6 h of ischemia, and 24 h of ischemia). Cerebral ischemia was achieved by unilateral ligating of CCA and ECA at the same time. After surgical preparation and awaiting for appropriate ischemia time we collected blood and brain tissue samples. Then we investigated serum occludin, claudin-5 and JAM-A levels from blood samples and the apoptotic index and percentages of pycnotic nucleus from brain tissues histologically. The obtained data were analyzed using IBM SPSS Statistics software package version 18 and the Jamovi software package.

Results: Serum JAM-A level showed a statistically significant difference in all ischemia groups compared with the control group (p<0.05). Serum claudin-5 level, a statistically significant difference was found between the control group and the 6-h ischemia group (p<0.05), while no significant inter-group differences were determined for the serum occludin level. As a result, in our experimental focal cerebral ischemia model, serum JAM-A showed more significant and rapid increases compared to occluding and claudin-5. (Since four rats of the 24-h ischemia group died before completing their term, these group data were not statistically evaluated.)

Conclusions: Serum JAM-A might be successfully used in the early stages of ischemic stroke. The presence of hemiparesis or hemiplegic effects in all rat ischemia groups and the significant increases in pyknotic and apoptotic cell counts histologically suggest that our model is successful for focal cerebral ischemia.

Anahtar Kelimeler

Cerebral Ischemia, Junctional Adhesion Molecule-A, Occludin, Claudin-5, rats

Etik Beyan

This study was conducted according to the ARRIVE and Guide for the Care and Use of Laboratory Animals.

Destekleyen Kurum

The Committee for Scientific Researches of Adnan Menderes University

Proje Numarası

2020/TPF-20047

Teşekkür

The authors would like to thank Francisco Jose Lopez Junior, M.D. for his valuable support during the surgical manipulation of the rats.

Kaynakça

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