Oxidative Alteration in Gingival Fibroblast Cells Induced By Bulk-Fill and Conventional Flowable Composites

Year 2018, Volume: 19 Issue: 4, 302 - 309, 31.12.2018

Neslihan Çelik , Merve İşcan Yapar , Ali Taghizadehghalehjoughi

https://doi.org/10.4274/meandros.70299

Cited By: 1

Abstract

Objective: The release of components from dental materials may cause oxidative stress which is crucial factor for tissue damage and cell apoptosis or death. The aim of this study was to evaluate the cytotoxicity of different flowable composites and this materials effect on total antioxidant capacity (TAC) and total oxidant status (TOS) level in human gingival fibroblast cell culture.
Materials and Methods: Gingival fibroblast cells obtained from healthy persons were used for evaluation the cytotoxicity and oxidant status. Six flowable composites used were: two bulk-fill flowable composites (SureFil SDR, X-tra base), a self-adhering flowable composite (Vertise Flow), a highly filled flowable composite (GrandioSO Flow), two conventional flowable composites (Filtek Ultimate, Clearfil Majesty). Specimens in 3 mm diameter, 2 mm height were prepared from each composite (n=6) and were transferred to 24 well plates. Wells without composite material were used as the control group. After 24 h incubation period, cytotoxicity was determined by using the 3-(4,5 dimetylthiazol-2-yl)-2,5 diphenlytetrazolium bromide (MTT) assay. Oxidative alterations were assessed using TAC and TOS assay kits. Data were analyzed using the ANOVA and least significant differences posthoc test.
Results: Cytotoxicity of six materials was significantly different from the control group (p<0.05). Vertise flow was the most cytotoxic material. TAC levels of Vertise flow were significantly different from X-tra base and GrandioSO. TOS levels increased in SureFil SDR and Vertise flow groups but it was not statistically significant difference.
Conclusion: All of the materials used in this study showed cytotoxic effect in human gingival fibroblast cell culture. These materials did not have a significant effect on TOS level. However, TAC level could not prevent the rise of TOS level in Vertise and sureFil SDR

Keywords

Flowable composite , antioxidant capacity , cell culture , oxidative stress

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