The effects of exercise on vascular responses in rats with type 1 diabetes

Yıl 2024, Cilt: 9 Sayı: 1, 247 - 253, 12.06.2024

Zihni Can Cengiz Ünsal

Öz

Diabetes causes dysfunctions and damages in different tissues over time. It has been known that exercise has beneficial effects on the pathologies associated with diabetes. This study was aimed to evaluate the effects of moderate swimming exercise on vascular responses in rats with type 1 diabetes. The groups in the experiment were conducted as diabetes, diabetes exercise, exercise, and control. Streptozotocin (50 mg/kg) was intraperitoneally given to induce type 1 diabetes. The rats in group diabetes exercise and exercise were subjected to a swimming protocol was applied 5 days a week and 1 hour a day for 4 weeks after streptozotocin injection. The initial and final blood glucose levels and weekly body weights were measured. At the end of the study, in vitro thoracic aorta responses were recorded. A reduction in body weight of rats with type 1 diabetes was determined from week 1 to week 4 (p<0.001). Blood glucose levels were significantly (p<0.001) higher in both diabetic groups than those of controls and group exercise. The 4-week swimming exercise had no effect on blood glucose levels of diabetic rats. The responses of the thoracic aorta to norepinephrine and sodium nitroprusside were not different between groups. The control rats showed the highest relaxation response of the thoracic aorta to acetylcholine while this response gradually decreased in groups diabetes exercise, exercise, and diabetes. In conclusion, it was observed that 4-week moderate swimming exercise regimen corrected endothelium-dependent relaxation responses in rats with type 1 diabetes.

Anahtar Kelimeler

acetylcholine, aorta, exercise, phenylephrine, rat, type 1 diabetes

Etik Beyan

The study was approved by the Aydın Adnan Menderes University Animal Experiments Local Ethics Committee (approval number: 64583101/2014/142).

Proje Numarası

VTF-15021.

Teşekkür

This study was presented as an oral presentation at the 42nd National Physiology Congress of Turkish Physiological Sciences Association (Düzce University Congress Center 05-08 September 2016). The abstract of the presentation is published in the Congress Proceedings and ACTA Physiologica Volume 215, Issue S704. In addition, the serum samples obtained from the study were analyzed and presented (Oral Presentation) with the title “The Effect of Swimming Exercise on Lipid Profile and Oxidant-Antioxidant Status in Type I Diabetic Rats” in 10th The 10th International Conference in Physical Education, Sports and Physical Therapy (18-20 November 2016 Fırat University, Faculty of Sport Sciences, Elazig). This chapter was published in “F.Ü. Sağ. Bil. Tıp Derg. 2016; 30 (3): 119-123” as full text.

Kaynakça

• Akata, T. (2007). General anesthetics and vascular smooth muscle: direct actions of general anesthetics on cellular mechanisms regulating vascular tone. Anesthesiology, 106(2), 365-391. https://doi.org/10.1097/00000542-200702000-00026
• Alberti, K. G., & Zimmet, P. Z. (1998). Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabetic Medicine, 15(7), 539-553. https://doi.org/10.1002/(SICI)1096-9136(199807)15:7<539::AID-DIA668>3.0.CO;2-S
• Bonaventura, D., Lunardi, C. N., Rodrigues, G. J., Neto, M. A., & Bendhack, L. M. (2008). A novel mechanism of vascular relaxation induced by sodium nitroprusside in the isolated rat aorta. Nitric Oxide, 18(4), 287-295. https://doi.org/10.1016/j.niox.2008.02.004
• Brownlee, M. (2001). Biochemistry and molecular cell biology of diabetic complications. Nature, 414(6865), 813-820. https://doi.org/10.1038/414813a
• Carmines, P. K., Ohishi, K., & Ikenaga, H. (1996). Functional impairment of renal afferent arteriolar voltage-gated calcium channels in rats with diabetes mellitus. Journal of Clinical Investigation, 98(11), 2564-2571. https://doi.org/10.1172/JCI119075
• Chimen, M., Kennedy, A., Nirantharakumar, K., Pang, T. T., Andrews, R., & Narendran, P. (2012). What are the health benefits of physical activity in type 1 diabetes mellitus? A literature review. Diabetologia, 55(3), 542-551. https://doi.org/10.1007/s00125-011-2403-2
• Dai, F. X., Diederich, A., Skopec, J., & Diederich, D. (1993). Diabetes-induced endothelial dysfunction in streptozotocin-treated rats: role of prostaglandin endoperoxides and free radicals. Journal of the American Society of Nephrology, 4(6), 1327-1336. http://www.ncbi.nlm.nih.gov/pubmed/8130359
• Di Francescomarino, S., Sciartilli, A., Di Valerio, V., Di Baldassarre, A., & Gallina, S. (2009). The effect of physical exercise on endothelial function. Sports Medicine, 39(10), 797-812. https://doi.org/10.2165/11317750-000000000-00000
• Donmez, S., Gokalp, O., Dogan, M., Vural, H., & Yigit, B. (2014). Effects of short-term hyperglycemia on the vasoconstriction of the aorta. Turkish Journal of Medicine and Science, 44(6), 941-945. http://www.ncbi.nlm.nih.gov/pubmed/25552145
• Fulton, D. J., Hodgson, W. C., Sikorski, B. W., & King, R. G. (1991). Attenuated responses to endothelin-1, KCl and CaCl2, but not noradrenaline, of aortae from rats with streptozotocin-induced diabetes mellitus. British Journal of Pharmacology, 104(4), 928-932. https://doi.org/10.1111/j.1476-5381.1991.tb12528.x
• Garber, C. E., Blissmer, B., Deschenes, M. R., Franklin, B. A., Lamonte, M. J., Lee, I. M., Nieman, D. C., Swain, D. P., & American College of Sports, M. (2011). American College of Sports Medicine position stand. Quantity and quality of exercise for developing and maintaining cardiorespiratory, musculoskeletal, and neuromotor fitness in apparently healthy adults: guidance for prescribing exercise. Medicine and Science in Sports and Exercise, 43(7), 1334-1359. https://doi.org/10.1249/MSS.0b013e318213fefb
• Hall, K. E., McDonald, M. W., Grise, K. N., Campos, O. A., Noble, E. G., & Melling, C. W. (2013). The role of resistance and aerobic exercise training on insulin sensitivity measures in STZ-induced Type 1 diabetic rodents. Metabolism, 62(10), 1485-1494. https://doi.org/10.1016/j.metabol.2013.05.012
• Joshi, A., & Woodman, O. L. (2012). Increased nitric oxide activity compensates for increased oxidative stress to maintain endothelial function in rat aorta in early type 1 diabetes. Naunyn Schmiedebergs Archives of Pharmacology, 385(11), 1083-1094. https://doi.org/10.1007/s00210-012-0794-3
• Karasu, C., & Altan, V. M. (1993). The role of endothelial cells on the alterations in vascular reactivity induced by insulin-dependent diabetes mellitus: effects of insulin treatment. General Pharmacology, 24(3), 743-755. http://www.ncbi.nlm.nih.gov/pubmed/8365655
• Keegan, A., Walbank, H., Cotter, M. A., & Cameron, N.E. (1995). Chronic vitamin E treatment prevents defective endothelium-dependent relaxation in diabetic rat aorta. Diabetologia, 38(12), 1475-1478. http://www.ncbi.nlm.nih.gov/pubmed/8786022
• Kiraly, M. A., Bates, H. E., Yue, J. T., Goche-Montes, D., Fediuc, S., Park, E., Matthews, S. G., Vranic, M., & Riddell, M. C. (2007). Attenuation of type 2 diabetes mellitus in the male Zucker diabetic fatty rat: the effects of stress and non-volitional exercise. Metabolism, 56(6), 732-744. https://doi.org/10.1016/j.metabol.2006.12.022
• Kobayashi, T., & Kamata, K. (1999). Effect of insulin treatment on smooth muscle contractility and endothelium-dependent relaxation in rat aortae from established STZ-induced diabetes. British Journal of Pharmacology, 127(4), 835-842. https://doi.org/10.1038/sj.bjp.0702554
• Kolluru, G. K., Bir, S. C., & Kevil, C. G. (2012). Endothelial dysfunction and diabetes: effects on angiogenesis, vascular remodeling, and wound healing. International Journal of Vascular Medicine, 2012. https://doi.org/10.1155/2012/918267
• Kregel, K. C. (2006). Resource book for the design of animal exercise protocols. American Physiological Society.
• Leo, C. H., Joshi, A., & Woodman, O. L. (2010). Short-term type 1 diabetes alters the mechanism of endothelium-dependent relaxation in the rat carotid artery. American Journal of Physiology Heart and Circulatory Physiology, 299(2), H502-H511. https://doi.org/10.1152/ajpheart.01197.2009
• Leung, H. S., Leung, F. P., Yao, X., Ko, W. H., Chen, Z. Y., Vanhoutte, P. M., & Huang, Y. (2006). Endothelial mediators of the acetylcholine-induced relaxation of the rat femoral artery. Vascular Pharmacology, 44(5), 299-308. https://doi.org/10.1016/j.vph.2006.01.010
• Minami, A., Ishimura, N., Harada, N., Sakamoto, S., Niwa, Y., & Nakaya, Y. (2002). Exercise training improves acetylcholine-induced endothelium-dependent hyperpolarization in type 2 diabetic rats, Otsuka Long-Evans Tokushima fatty rats. Atherosclerosis, 162(1), 85-92. https://doi.org/10.1016/s0021-9150(01)00685-2
• Mona, M. A., & Allam, M. H. (2018). Effects of moderate exercise training and detraining on diabetic peripheral neuropathy in streptozotocin-induced diabetic rats. Benha Medical Journal, 35, 150-156.
• Nakos, I., Kadoglou, N. P. E., Gkeka, P., Tzallas, A. T., Giannakeas, N., Tsalikakis, D. G., Katsimpoulas, M., Mantziaras, G., Kostomitsopoulos, N., Liapis, C. D., & Kakisis, J. (2018). Exercise training attenuates the development of cardiac autonomic dysfunction in diabetic rats. In Vivo, 32(6), 1433-1441. https://doi.org/10.21873/invivo.11396
• Oyama, Y., Kawasaki, H., Hattori, Y., & Kanno, M. (1986). Attenuation of endothelium-dependent relaxation in aorta from diabetic rats. European Journal of Pharmacology, 132(1), 75-78. https://doi.org/10.1016/0014-2999(86)90013-0
• Ratamess, N. A., Alvar, B. A., Evetoch, T. E., Housh, T. J., Kibler, W. B., Kraemer, W. J., & Triplett, T. (2009). American College of Sports Medicine position stand. Progression models in resistance training for healthy adults. Medicine and Science in Sports and Exercise, 41(3), 687-708. https://doi.org/10.1249/MSS.0b013e3181915670
• Taylor, P. D., McCarthy, A. L., Thomas, C. R., & Poston, L. (1992). Endothelium-dependent relaxation and noradrenaline sensitivity in mesenteric resistance arteries of streptozotocin-induced diabetic rats. British Journal of Pharmacology, 107(2), 393-399. https://doi.org/10.1111/j.1476-5381.1992.tb12757.x
• Taylor, P. D., Oon, B. B., Thomas, C. R., & Poston, L. (1994). Prevention by insulin treatment of endothelial dysfunction but not enhanced noradrenaline-induced contractility in mesenteric resistance arteries from streptozotocin-induced diabetic rats. British Journal of Pharmacology, 111(1), 35-41. https://doi.org/10.1111/j.1476-5381.1994.tb14020.x
• Tonoli, C., Heyman, E., Roelands, B., Buyse, L., Cheung, S. S., Berthoin, S., & Meeusen, R. (2012). Effects of different types of acute and chronic (training) exercise on glycaemic control in type 1 diabetes mellitus: a meta-analysis. Sports Medicine, 42(12), 1059-1080. https://doi.org/10.2165/11635380-000000000-00000
• Torgan, C. E., Brozinick, J. T., Jr., Banks, E. A., Cortez, M. Y., Wilcox, R. E., & Ivy, J. L. (1993). Exercise training and clenbuterol reduce insulin resistance of obese Zucker rats. American Journal of Physiology, 264(3 Pt 1), E373-379. https://doi.org/10.1152/ajpendo.1993.264.3.E373
• Turgut, N. H., Temiz, T. K., Bagcivan, I., Turgut, B., Gulturk, S., & Karadas, B. (2008). The effect of sildenafil on the altered thoracic aorta smooth muscle responses in rat pre-eclampsia model. European Journal of Pharmacology, 589(1-3), 180-187. https://doi.org/10.1016/j.ejphar.2008.04.034
• Ünsal, C., & Ünsal, H. (2016). Tip I diyabetik sıçanlarda yüzme egzersizinin lipid profili ve oksidan-antioksidan duruma etkisi. Fırat Üniversitesi Sağlık Bilimleri Dergisi, 30(3), 119-123.
• Ünsal, H., Ünsal, C., Üner, A. G., Koç Yıldırım, E., Balkaya, M., & Belge, F. (2017). The effects of swimming exercise and probiotic VSL#3 on zonulin and some inflammatory and oxidative parameters in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(1), 101-107. https://doi.org/10.9775/kvfd.2016.15949
• Wang, R., Wu, Y., Tang, G., Wu, L., & Hanna, S. T. (2000). Altered L-type Ca(2+) channel currents in vascular smooth muscle cells from experimental diabetic rats. American Journal of Physiology-Heart and Circulatory Physiology, 278(3), H714-722. https://doi.org/10.1152/ajpheart.2000.278.3.H714
• Yardley, J. E., Kenny, G. P., Perkins, B. A., Riddell, M. C., Balaa, N., Malcolm, J., Boulay, P., Khandwala, F., & Sigal, R. J. (2013). Resistance versus aerobic exercise: acute effects on glycemia in type 1 diabetes. Diabetes Care, 36(3), 537-542. https://doi.org/10.2337/dc12-0963