First detection and molecular characterization of Felis catus papillomavirus in Türkiye

Year 2025, Volume: 10 Issue: 2, 504 - 512, 31.08.2025

Nüvit Coşkun , Emin Karakurt , Fırat Doğan , Volkan Yılmaz , Uğur Aydın , Veysel Soydal Ataseven , Serpil Dağ , Özgür Aksoy , İsa Özaydın , Ayfer Yıldız , Uğur Yıldız , Ersin Tanrıverdi

https://doi.org/10.24880/meditvetj.1705188

Abstract

Papillomaviruses have a wide range of hosts including human and animals. It is known that papillomaviruses alter cell regulation pathways. For this reason, they are known to be responsible for some of human cancers and they have been associated with neoplastic diseases in different animal species. Although many cases of papillomaviruses have been described in most domestic species they are not as frequently detected in cats. In this study we investigated 7 cases of cats with lesions for papillomavirus presence. Biopsy samples were initially examined histopathologically for diagnosis. After routine procedures all samples were diagnosed as squamous cell carcinomas (SCC). For papillomavirus nucleic acid detection in all samples were investigated by polymerase chain reaction with primers specific to L1 gene of papillomavirus using MY09/11 and FAP59/64 primer pairs. Papillomavirus DNA was found in one of the seven samples. The positive sample was sequenced and subjected to phylogenetical analysis. The sample was classified as Felis catus papillomavirus type 3 (FcaPV-3). This study reports the molecular presence of feline papillomaviruses in Türkiye for the first time and characterizes as FcaPV-3 genotype.

Keywords

Feline papillomavirus , Histopathology , Polymerase chain reaction , Phylogenetical analysis , Viral diagnosis

Türkiye'de Felis catus papillomavirusun ilk tespiti ve moleküler karakterizasyonu

Abstract

Papillomaviruslar insan ve hayvanları da içeren geniş bir konakçı yelpazesine sahiptir. Papillomavirusların hücre düzenleme yolaklarını değiştirdiği bilinmektedir. Bu nedenle, insan kanserlerinin bazılarından sorumlu oldukları bilinmektedir ve farklı hayvan türlerinde neoplastik hastalıklarla ilişkilendirilmişlerdir. Birçok evcil türde papillomavirus vakaları tanımlanmış olmasına rağmen, kedilerde bu kadar sık tespit edilmemiştir. Bu çalışmada, lezyonlu 7 kedi vakası papillomavirus varlığı açısından araştırılmıştır. Biyopsi örnekleri ilk olarak tanı için histopatolojik olarak incelendi. Rutin prosedürlerden sonra tüm örnekler skuamöz hücreli karsinom (SCC) olarak teşhis edildi. Tüm örneklerde papillomavirus nükleik asit tespiti için MY09/11 ve FAP59/64 primer çiftleri kullanılarak papillomavirusun L1 genine özgü primerlerle polimeraz zincir reaksiyonu ile araştırılmıştır. Yedi örnekten birinde papillomavirus DNA'sı bulunmuştur. Pozitif örnek dizilenmiş ve filogenetik analize tabi tutulmuştur. Örnek Felis catus papillomavirus tip 3 (FcaPV-3) olarak sınıflandırılmıştır. Bu çalışma, Türkiye'de kedi papillomavirusun moleküler varlığını ilk kez bildirmekte ve FcaPV-3 genotipi olarak karakterize etmektedir.

Keywords

Feline papillomavirus , Filogenetik analiz , Histopatoloji , Polimeraz zincir reaksiyonu , Viral tanı

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