IN-VITRO INVESTIGATION OF DONKEY MILK EFFICACY AGAINST STANDARD STAPHYLOCOCCUS AUREUS STRAINS

Year 2021, , 145 - 149, 30.12.2021

Akin Yığın , Mehmet Demirci , Serap Kılıç Altun , Bekir Kocazeybek

https://doi.org/10.51477/mejs.1014934

Abstract

has been used for therapeutic purposes since ancient times. Different studies report its antimicrobial activity against an important human pathogen such as S. aureus. Therefore, in our study, we aimed to examine the antimicrobial efficacy of donkey milk against standard MRSA and MSSA strains in-vitro environment. In our study, in-vitro antimicrobial efficacy of donkey milk obtained from 53 different donkeys against S. aureus ATCC 43300 (MRSA) and S. aureus ATCC 29213 (MSSA) was investigated by broth microdilution method. Donkey milk mixed with TSB medium at different serial dilution rates was studied in 96 microwell plates. When the effectiveness of different concentrations of donkey milk added to TSB medium in vitro against ATCC MRSA and MSSA strains was examined, no suppression was observed, and growth was found to be higher than the control results after 24 hours of incubation at high milk concentrations. In conclusion, according to the results of our study, no antimicrobial effect of donkey milk was observed in-vitro against standard MRSA and MSSA strains. This situation may be caused by the fact that S. aureus strains may have different virulence abilities, as well as the differences in protein structure in milk between species.

Keywords

donkey milk, staphylococcus aureus, milk, MRSA, MSSA

References

• Derdak R, Sakoui S, Pop OL, et al. “Insights on Health and Food Applications of Equus asinus (Donkey) Milk Bioactive Proteins and Peptides-An Overview”. Foods. 2020;9(9):1302. doi:10.3390/foods
• Martini M., Altomonte I., Licitra R., Salari F. “Nutritional and Nutraceutical Quality of Donkey Milk”. J. Equine Vet. Sci. 2018;65:33–37. doi: 10.1016/j.jevs.2017.10.020
• Osman Swar M. “Donkey milk-based formula: A substitute for patients with cow's milk protein allergy”. Sudan J Paediatr. 2011;11(2):21-24.
• Gordon RJ, Lowy FD. “Pathogenesis of methicillin-resistant Staphylococcus aureus infection”. Clin Infect Dis. 2008;46 Suppl 5(Suppl 5):S350-S359. doi:10.1086/533591
• Branger C, Gardye C, Galdbart JO, Deschamps C, Lambert N. “Genetic relationship between methicillin-sensitive and methicillin-resistant Staphylococcus aureus strains from France and from international sources: delineation of genomic groups”. J Clin Microbiol. 2003;41(7):2946-2951. doi:10.1128/jcm.41.7.2946-2951.2003
• David MZ, Boyle-Vavra S, Zychowski DL, Daum RS. “Methicillin-susceptible Staphylococcus aureus as a predominantly healthcare-associated pathogen: a possible reversal of roles? ”. PLoS One. 2011;6(4):e18217. doi:10.1371/journal.pone.0018217
• Yirmibeşoğlu SSS, Öztürk BET. “Comparing microbiological profiles, bioactivities, and physicochemical and sensory properties of donkey milk kefir and cow milk kefir”. Turkish Journal of Veterinary and Animal Sciences 2020;44 (4):774-781.
• Koutb M, Khider M, Ali EH et al. “Antimicrobial Activity of Donkey Milk Against Dermatomycotic Fungi and Foodborne Bacteria”. International Journal of Biomedical Materials Research. 2016; 4:11-17. doi: 10.11648/j.ijbmr.20160403.11
• Dinç H, Demirci M, Yiğin A. “Lactobacillus acidophilus ve Lactobacillus casei’nin, metisilin dirençli ve metisilin duyarlı Staphylococcus aureus biyofilm genlerine in-vitro etkisinin incelenmesi”. Eurasian Journal of Veterinary Sciences, 35(1), 44-48. 2019.
• Demirci M, Yigin A, Altun SK, Uysal HK, Saribas S, Kocazeybek BS. “Salmonella Spp. and Shigella Spp. detection via multiplex real-time PCR and discrimination via MALDI-TOF MS in different animal raw milk samples”. Niger J Clin Pract. 2019 Aug;22(8):1083-1090. doi: 10.4103/njcp.njcp_596_18. Kesici S, Demırci M, Kesici U. “Efeitos antimicrobianos do fentanil e da bupivacaína: estudo in vitro [Antimicrobial effects of fentanyl and bupivacaine] ”. Rev Bras Anestesiol. 2020 Jul-Aug;70(4):357-363. Portuguese. doi: 10.1016/j.bjan.2020.04.010.
• Assiri AMA, Elbanna K, Al-Thubiani A. et al. “Cold-pressed oregano (Origanum vulgare) oil: a rich source of bioactive lipids with novel antioxidant and antimicrobial properties”. Eur Food Res Technol. 242, 1013–1023 (2016). doi:10.1007/s00217-015-2607-7
• Šarić, L., Pezo, L., Šarić, B. et al. “Calcium-dependent antibacterial activity of donkey’s milk against Salmonella”. Ann Microbiol. 67, 185–194 (2017). doi:10.1007/s13213-016-1250-2
• Brumini, D., Criscione, A., Bordonaro, S. et al. “Whey proteins and their antimicrobial properties in donkey milk: a brief review”. Dairy Sci. & Technol. 96, 1–14 (2016). doi:10.1007/s13594-015-0246-1
• Ljubiša Šarić et al., “Antibacterial activity of Domestic Balkan donkey milk toward Listeria monocytogenes and Staphylococcus aureus”, Food and Feed Research, 41(1), 47-54, 2014.
• Pilla, R., Daprà, V., Zecconi, A., Piccinini, R., “Hygienic and health characteristics of donkey milk during a follow-up study”, J Dairy Res, 77(4), 392-7, 2010. doi: 10.1017/S0022029910000221.