PHYTOCHEMICAL PROFILING BY LC-ESI-MS/MS AND DETERMINATION OF ANTIOXIDANT, ANTI-ALZHEIMER AND ANTITYROSINASE ACTIVITIES OF TWO ASPARAGACEAE SPECIES: Scilla hyacinthoides L. AND Scilla ingridiae SPETA

Abstract

In this study, in vitro antioxidant, antialzheimer and antityrosinase activity, total phenolic and flavonoid components, phytochemical profiling, identified by Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) analysis and also chemometric analysis, of two different parts of Scilla species (Scilla hyacinthoides L. and Scilla ingridiae Speta) were determined in detailed perspective. The highest contents of total phenolics (TPC) and total flavonoids (TFC) were determined in S1TU (S. hyacinthoides aerial parts) extract (11.72±0.00 µg GAE/mg) and S2TU (S. ingridiae aerial parts) extract (31.53±0.21 µg QE/mg). The highest ABTS and CUPRAC activities were found in S1TU and S2TU extracts. The inhibitory activities of the extracts on the enzyme acetylcholinesterase were investigated. S1TU and S2TU extracts again showed the highest activity. Although the antityrosinase enzyme inhibitory activities of the extracts were generally similar and high, the S2TA extract (S. ingridiae corm parts) showed the highest activity. LC-ESI-MS/MS was used to determine the content of phenolic components of the extracts. Fourteen different bioactive components were determined in the analyzes and their amounts were measured. The data obtained were analyzed chemometrically such as principal component analysis (PCA), hierarchical component analysis (HCA) and their relationships to each other were supported visually and numerically with Pearson correlation graphs, heat maps, etc. The research results have shown that the various components of the plant have a good effect on various biological activities, so that it can be used for various purposes in the future (especially with a good degree of antityrosinase activity), taking into account the results. The biological activities demonstrated here can rationalize the use of the plant in traditional medicine.

Keywords

• Antioxidant activity
• phytochemical profiling
• enzyme inhibition
• multivariate analysis
• Pearson correlation

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