Investigation of BSA adsorption performances of metal ion attached mineral particles embedded cryogel discs

Yıl 2021, , 65 - 71, 30.04.2021

Ömür Acet

https://doi.org/10.51354/mjen.883804

Cited By: 1

Öz

 Blood plasma is rich in albumin protein. Albumin has some physiological duties. Investigations over separation of albumin has been paid considerable interest for its excellent potential in blood protein production. Natural pumice particles are non-toxic, reasonably priced and alternative adsorbents with excellent adsorption performance. In order to study the adsorption performance of BSA on composite cryogel discs with immobilized metal affinity chromatography (IMAC), Cu+2-attached natural pumice particles were designed and they were embedded into cryogel generated medium. Cu2+-attached natural pumice particle embedded composite cryogel discs (Cu2+-NP-ECDs) were synthesized through polymerization of gel-former factors at minus temperatures. The characterization experiments of the Cu2+-NP-ECDs were accomplished via SEM, FTIR experiments. The experiments were studied in a batch system. The highest amount of adsorbed BSA (356,8 mg/g particles) onto discs was obtained at pH 7.0 (phosphate buffer), 4 mg/mL concentration of BSA. As a result of conducted 30 adsorption-desorption experiments periods, there was no important change in adsorption performance of composite discs.

Anahtar Kelimeler

IMAC, cryogel disc, pumice particle, bovine serum albumin, adsorption

Kaynakça

• [1] Hinderliter, Anne, Sylvio May., “Cooperative adsorption of proteins onto lipid membranes”, Journal of Physics: Condensed Matter 18 (28), (2006), S1257.
• [2] Horbett, T. A., Brash, J. L., “Proteins at interfaces II”, Washington, DC: American Chemical Society, 1995, pp. 580. [3] Rauf, T. A., Anirudhan, T. S., “Synthesis and characterization of sulphonic acid ligand immobilized Aminopropyl silanetriol copolymer and evaluation of its Bovine serum albumin adsorption efficiency”, Materials Today: Proceedings, (2020).
• [4] Zhang, J., Zhang, Z., Song, Y., Cai, H., “Bovine serum albumin (BSA) adsorption with Cibacron Blue F3GA attached chitosan microspheres”, Reactive and Functional Polymers, 66(9), (2006), 916-923.
• [5] Ràfols, C., Zarza, S., Bosch, E. “Molecular interactions between some non-steroidal anti-inflammatory drugs (NSAID׳ s) and bovine (BSA) or human (HSA) serum albumin estimated by means of isothermal titration calorimetry (ITC) and frontal analysis capillary electrophoresis (FA/CE)”, Talanta, 130, (2014), 241-250.
• [6] Hage, D. S., “Affinity chromatography: a review of clinical applications”, Clinical chemistry, 45(5), (1999), 593-615.
• [7] Bourassa, P., Kanakis, C. D., Tarantilis, P., Pollissiou, M. G., Tajmir-Riahi, H. A., “Resveratrol, genistein, and curcumin bind bovine serum albümin”, The Journal of Physical Chemistry B, 114(9), (2010), 3348-3354.
• [8] Shah, M. T., Alveroglu, E., “Facile synthesis of nanogels modified Fe3O4@ Ag NPs for the efficient adsorption of bovine & human serum albümin”, Materials Science and Engineering: C, 118, (2021), 111390.
• [9] Acet, Ö., Aksoy, N. H., Erdönmez, D., Odabaşı, M., “Determination of some adsorption and kinetic parameters of α-amylase onto Cu+ 2-PHEMA beads embedded column”, Artificial cells, nanomedicine, and biotechnology, 46(sup3), (2018), S538-S545.
• [10] Acet, Ö., Menteş, A., Odabaşı, M., “Assessment of a new dual effective combo polymer structure for separation of lysozyme from hen egg white”, Polymer Bulletin, (2019), 1-17.
• [11] Serinbaş, A., Önal, B., Acet, Ö., Özdemir, N., Dzmitruk, V., Halets-Bui, I., Shcharbin, D., Odabaşı, M. (2020). “A new application of inorganic sorbent for biomolecules: IMAC practice of Fe3+-nano flowers for DNA separation”, Materials Science and Engineering: C, 113, 111020.
• [12] Önal, B., Acet, Ö., Sanz, R., Sanz-Pérez, E. S., Erdönmez, D., Odabaşı, M. “ Co-evaluation of interaction parameters of genomic and plasmid DNA for a new chromatographic medium”, International journal of biological macromolecules, 141, (2019), 1183-1190.
• [13] Alacabey, İ., Acet, Ö., Önal, B., Dikici, E., Karakoç, V., Gürbüz, F., Alkan, H., Odabaşı, M., “Pumice particle interface: a case study for immunoglobulin G purification”, Polymer Bulletin, (2020), 1-15.
• [14] Li, S., Yang, K., Liu, L., Zhao, B., Chen, Y., Li, X., Zhang, L., Zhang, Y. (2018). “Surface sieving coordinated IMAC material for purification of His-tagged proteins”, Analytica chimica acta, 997, 9-15.
• [15] Rezaeeyazdi, M., Colombani, T., Memic, A., Bencherif, S. A., “ Injectable hyaluronic acid-co-gelatin cryogels for tissue-engineering applications”, Materials, 11(8), (2018),1374.
• [16] Hixon, K. R., Lu, T., Sell, S. A., “A comprehensive review of cryogels and their roles in tissue engineering applications”, Acta biomaterialia, 62, (2017), 29-41.
• [17] Eggermont, L. J., Rogers, Z. J., Colombani, T., Memic, A., Bencherif, S. A. “Injectable cryogels for biomedical applications”, Trends in biotechnology, 38(4), (2020), 418-431.
• [18] Önal, B., Odabaşı, M., “Design and application of a newly generated bio/synthetic cryogel column for DNA capturing”, Polymer Bulletin, (2020), 1-18.
• [19] Ceylan, Ş., Odabaşı, M., “Novel adsorbent for DNA adsorption: Fe3+-attached sporopollenin particles embedded composite cryogels”, Artificial cells, nanomedicine, and biotechnology, 41(6), (2013), 376-383.
• [20] Huseynli, S., Bakhshpour, M., Qureshi, T., Andac, M., Denizli, A., “Composite polymeric cryogel cartridges for selective removal of cadmium ions from aqueous solutions”, Polymers, 12(5), (2020), 1149.
• [21] Gurbuz, F., Ozcan, A., Çiftçi, H., Acet, O., Odabasi, M., “Treatment of textile effluents through bio-composite column: Decolorization and COD reduction”, International Journal of Environmental Science and Technology, 16(12), (2019), 8653-8662.
• [22] Gurbuz, F., Akpınar, Ş., Ozcan, S., Acet, Ö., Odabaşı, M., “Reducing arsenic and groundwater contaminants down to safe level for drinking purposes via Fe 3+-attached hybrid column”, Environmental monitoring and assessment, 191(12), (2019), 1-14.
• [23] Persson, P., Baybak, O., Plieva, F., Galaev, I. Y., Mattiasson, B., Nilsson, B., Axelsson, A., “Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles”, Biotechnology and bioengineering, 88(2), (2004), 224-236.
• [24] Ünlü, N., Ceylan, Ş., Erzengin, M., & Odabaşı, M., “Investigation of protein adsorption performance of Ni2+‐attached diatomite particles embedded in composite monolithic cryogels”, Journal of separation science, 34(16‐17), (2011), 2173-2180.
• [25] Bereli, N., Şener, G., Altıntaş, E. B., Yavuz, H., Denizli, A., “Poly (glycidyl methacrylate) beads embedded cryogels for pseudo-specific affinity depletion of albumin and immunoglobulin G”, Materials Science and Engineering: C, 30(2), (2010), 323-329.
• [26] Yao, K., Yun, J., Shen, S., Wang, L., He, X., Yu, X., “Characterization of a novel continuous supermacroporous monolithic cryogel embedded with nanoparticles for protein chromatography”, Journal of Chromatography A, 1109(1), (2006), 103-110.
• [27] Gurbuz, F., Ceylan, Ş., Odabaşı, M., Codd, G. A., “Hepatotoxic microcystin removal using pumice embedded monolithic composite cryogel as an alternative water treatment method”, Water research, 90, (2016), 337-343.
• [28] Wang, X., Herting, G., Wallinder, I. O., Blomberg, E., “ Adsorption of bovine serum albumin on silver surfaces enhances the release of silver at pH neutral conditions ”, Physical Chemistry Chemical Physics, 17(28), (2015), 18524-18534.
• [29] Odabašı, M., Uzun, L., Denizli, A., “Porous magnetic chelator support for albumin adsorption by immobilized metal affinity separation”, Journal of applied polymer science, 93(5), (2004), 2501-2510.