Investigation of BSA adsorption performances of metal ion attached mineral particles embedded cryogel discs
Abstract
Blood plasma is rich in albumin protein. Albumin has some physiological duties. Investigations over separation of albumin has been paid considerable interest for its excellent potential in blood protein production. Natural pumice particles are non-toxic, reasonably priced and alternative adsorbents with excellent adsorption performance. In order to study the adsorption performance of BSA on composite cryogel discs with immobilized metal affinity chromatography (IMAC), Cu+2-attached natural pumice particles were designed and they were embedded into cryogel generated medium. Cu2+-attached natural pumice particle embedded composite cryogel discs (Cu2+-NP-ECDs) were synthesized through polymerization of gel-former factors at minus temperatures. The characterization experiments of the Cu2+-NP-ECDs were accomplished via SEM, FTIR experiments. The experiments were studied in a batch system. The highest amount of adsorbed BSA (356,8 mg/g particles) onto discs was obtained at pH 7.0 (phosphate buffer), 4 mg/mL concentration of BSA. As a result of conducted 30 adsorption-desorption experiments periods, there was no important change in adsorption performance of composite discs.
Keywords
IMAC, cryogel disc, pumice particle, bovine serum albumin, adsorption
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