Determination Of Escherichia Coli O157:h7 In Chicken Meats Sold In Şanlıurfa Region

Year 2016, Volume: 4 Issue: 1, 63 - 68, 01.05.2016

A. Dinçoğlu Z. Gönülalan

Abstract

Escherichia coli O157:H7 has been an important problem of public health in most countries of the world since 1982. This study was therefore aimed to investigate the presence of E. coli O157:H7 in chicken meat samples collected from various markets in Şanlıurfa region which was located in Southern Turkey. For this purpose, 155 chicken meat samples were analyzed between September 2005 and February 2006. The samples were plated onto CT-SMAC after enrichment process. Suspected colonies were then subjected for identification of E. coli O157:H7 given in materials and methods section. E.coli O157 and E.coli O157:H7 was found in 9 (5.81%) and 3 (1.94%) of total 155 samples, respectively. The results showed that control measures should be developed to prevent contamination with this pathogen in chicken meats in this region. To our knowledge, this is the first report of isolation of these bacteria from chicken meat samples in Southern Turkey.

Keywords

E.coli O157:H7, prevalence, chicken meat, cultural method, public health

Şanlıurfa Bölgesinde Satılan Tavuk Etlerinde Escherichia coli O157:H7’nin Tespiti

Abstract

Escherichia coli O157:H71982’den beri dünyanın birçok ülkesinde önemli bir halk sağlığı problemi olmuştur. Bu nedenle, bu çalışma Türkiye’nin güneyinde yer alan Şanlıurfa bölgesindeki çeşitli marketlerden toplanan tavuk eti örneklerinde E. coli O157:H7’nin varlığını araştırmak amacıyla yapıldı. Bu amaçla, 2005 Eylül ile 2006 Şubat döneminde toplanan 155 adet tavuk eti örneği analiz edildi. Zenginleştirme işleminden sonra örnekler Cefixime Tellurite Supplement içeren Sorbitol Mac Conkey Agara aktarıldı. Daha sonra şüpheli koloniler E. coli O157:H7’nin identifikasyonu için materyal ve metot kısmında belirtildiği gibi analiz edildi. Toplam 155 örneğin 9’unda (%5.81) E. coli O157 ve 3’ünde (%1.94) E. coli O157:H7 tespit edildi. Bu sonuçlar, bu bölgedeki tavuk etlerinin bu patojenle kontaminasyonunu önlemek için kontrol önlemlerinin artırılması gerektiğini gösterdi. Bildiğimiz kadarıyla, elde ettiğimiz sonuçlar Türkiye’nin güney bölgesindeki tavuk eti örneklerinden E. coli O157:H7’nin izole edildiği ilk rapordur

Keywords

Tavuk eti, kültürel metot, E. coli O157:H7, prevalans, halk sağlığı

References

• [1]. Fedio WM, Jinneman KC, Yoshitomi KJ, Zapata R, Wendakoon CN, Brownıng P and Weagant SD, Detection of E. coli O157:H7 in raw ground beef by PathatrixTM Immunomagnetic-separation, real-time PCR and cultural m methods. Int J Food Microbiol, 148, 87–92 (2011).
• [2]. Anonymous, Epidemiologic Notes and Reports Isolation of Escherichia coli O157:H7 from sporadic cases of hemorrhagic colitis, United States. CDC MMWR Weekly, 46(30), 700-704 (1997) http://www.cdc.gov/mmwr/preview/mmwrhtml/00048579.htm. [Accessed 25/11/2015]
• [3]. Riley LW, Remis RS, Helgerson SD, McGee HB and Wells JG, Hemorrhagic colitis associated with a rare Escherichia coli Serotype. N Engl J Med, 308, 681–685 (1983).
• [4]. Lim JY, Yoon J and Hovde CJ, A brief overview of Escherichia coli O157:H7 and its plasmid O157. J Microbiol Biotechnol, 20(1), 5-14 (2010).
• [5]. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM and Tauxe RV, Food-related illness and death in the United States. Emerging Infect Dis, 5, 607-625 (1999).
• [6]. Gordillo R, Cordoba JJ, Andrade MJ, Luque MI and Rodrıguez M, Development of PCR assays for detection of Escherichia coli O157:H7 in meat products. Meat Sci, 88, 767–773 (2011).
• [7]. Griffin PM, Escherichia coli O157:H7 and other enterohemorrhagic Escherichia coli. In “Infections of the gastrointestinal tract”, Blaser MJ, Smith PD, Ravdin JI, Greenberg HB and Guerrant RL, ed., Raven Press Ltd., New York, 1995, pp. 739–761.
• [8]. Kiranmayi B, Krishnaiah N, Naga Mallika E, Escherichia coli O157:H7 - An emerging pathogen in foods of animal origin. Vet World, 3(8), 382-389 (2010).
• [9]. Buchanan RL and Doyle MP, Foodborne disease significance of Escherichia coli O157:H7 and other enterohemorrhagic E. coli. Food Technology, 51(10), 69–76 (1997).
• [10]. Beery JT, Doyle MP and Schoeni JL, Colonization of Chicken Cecae by Escherichia coli Associated with Hemorrhagic Colitis. Appl Environ Microbiol, 49(2), 310-315 (1985).
• [11]. FDA (Food