Siyah havuç suyunun biyoaktif bileşenleri üzerine ultrases uygulamasının etkisi

Yıl 2025, Cilt: 30 Sayı: 2, 374 - 387, 21.08.2025

Erdi Ergene , Ömer Çakmak , Tuba Aldemir

https://doi.org/10.37908/mkutbd.1551541

Öz

Günümüzde tüketicilerin duyusal özellikleri korunarak besin içeriği zenginleştirilmiş doğal gıdalara olan bilinç düzeylerinin artması, yeni gıda uygulama proseslerine olan eğilimin artmasına yol açmıştır. Bu proseslerden biri olan ultrases, meyve sularının kalitesinin iyileştirilmesi için potansiyel bir teknik olarak kabul edilmektedir. Geleneksel ısı teknolojileri meyve sularının korunmasında yaygın kullanılmaktadır ancak istenmeyen değişimlere yol açmaktadır. Ultrases gibi termal olmayan teknolojiler, fonksiyonel özelliklerden ödün vermeden meyve sularının korunmasına alternatif olarak araştırılmaktadır. Siyah havuç sahip olduğu yüksek antioksidan kapasitesi, içerdiği antosiyanin pigmenti, polifenoller ve önemli mineraller ile dikkat çekmektedir. Bu çalışma; ultrases uygulamasının siyah havuç suyunun fiziko-kimyasal (pH değeri, asitlik ve Brix), toplam antioksidan kapasitesi, toplam fenolik madde, toplam flavonoidler ve toplam antosiyanin içeriği gibi bazı önemli kalite parametreleri üzerindeki etkisinin değerlendirilmesini amaçlamaktadır. Siyah havuç suyuna 20 kHz frekansta, %40 genlik değerinde sırasıyla 1,3,5,7 ve 10 dak. süreyle ultrases işlemi uygulanmıştır. Kontrol grubu olarak ayrılan gruba ise herhangi bir işlem uygulanmamıştır. Elde edilen bulgulara göre asitlik ve Briks değerlerinde önemli bir değişiklik görülmemiş ancak toplam flavonoid madde, toplam antioksidan ve toplam antosiyanin değerleri üzerinde olumlu yönde etki gösterdiği tespit edilmiştir. Tespit edilen artışın biyoaktif özellikler üzerinde olumlu bir etki oluşturduğu, en etkili sonucun 7 dak.’lık uygulama sonrasında elde edildiği belirlenmiştir.

Anahtar Kelimeler

Ultrases , Siyah havuç , Antosiyanin , Biyoaktif özellikler , Toplam fenolik madde

Destekleyen Kurum

İstanbul Esenyurt Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Teşekkür

Bu çalışma İstanbul Esenyurt Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından finansal olarak desteklenmiştir. (Proje No: BAP-2024/03-B)

Effect of ultrasound treatment on bioactive components of black carrot juice

Öz

Nowadays, the increasing awareness of consumers for natural foods with enriched nutritional content while preserving their sensory properties has led to an increased tendency towards new food application processes. Ultrasound is recognized as a possible technology for increasing the quality of fruit juices. Conventional heat technologies are widely used to preserve fruit juices, but they also lead to undesirable changes. Non-thermal technologies such as ultrasound are being investigated as an alternative to preserve fruit juices without compromising functional properties. Black carrot attracts attention with its high antioxidant capacity, anthocyanin pigment, polyphenols and essential minerals. This study aims to evaluate the effect of ultrasound treatment on some important quality parameters of black carrot juice, such as physico-chemical (pH value, acidity and Brix), total antioxidant capacity, total phenolic content, total flavonoids and total anthocyanin content. The black carrot juice was subjected to ultrasonic treatment at 20 kHz and 40% amplitude for 1,3,5,7 and 10 minutes, respectively. No treatment was applied to the control group. According to the results obtained, no significant change was observed in acidity and Brix values, but it was determined that it had a positive effect on total flavonoid matter, total antioxidant and total anthocyanin values.It was determined that the most effective result was obtained after 7 minutes of application, which positively affected bioactive properties.

Anahtar Kelimeler

Ultrasound , Black carrot , Anthocyanin , Bioactive properties , Total Phenolic substance

Kaynakça

• Aadil, R.M., Zeng, X.A., Han, Z., & Sun, D.W. (2013). Effects of ultrasound treatments on quality of grapefruit juice. Food Chemistry, 141, 3201-3206. https://doi.org/10.1016/j.foodchem.2013.06.008
• Abid, M., Jabbar, S., Wu, T., Hashim, M.M., Hu, B., Lei, S., Zhang, X., & Zeng, X. (2013). Effect of ultrasound on different quality parameters of apple juice. Ultrasonics Sonochemistry, 20 (5), 1182-1187. https://doi.org/10.1016/j.ultsonch.2013.02.010
• Adekunte, A.O., Tiwari, B.K., Cullen, P.J., Scannell, A.G.M., & O’Donnell, C.P. (2010). Effect of sonication on colour, ascorbic acid and yeast inactivation in tomato juice. Food Chemistry, 122 (3), 500-507. https://doi.org/10.1016/j.foodchem.2010.01.026
• Ağçam, E., & Akyıldız, A. (2015). Siyah havuç posasından antosiyaninlerin ekstraksiyonuna farklı çözgen ve asit konsantrasyonlarının etkileri. Gıda, 40 (3), 149-156. https://doi.org/10.15237/gida.GD14064
• Ağçam, E., Akyıldız, A., & Balasubramaniam, V.M. (2017). Optimization of anthocyanins extraction from black carrot pomace with thermosonication. Food Chemistry, 237, 461-470. https://doi.org/10.1016/j.foodchem.2017.05.098
• Akhan, M., Türkol, M., Yıkmış, S., Sancar, B.Ç., Çöl, B.G., Khalid, M.Z., Moreno, A., Khalid, W., & Alsulami, T. (2025). Enhancing the functionality and shelf life of poppy sherbet by optimizing ultrasound and propolis using response surface methodology: Impact on phenolic compounds, organic acids, sugar components, and sensory characteristics. LWT - Food Science and Technology, 218, 117453. https://doi.org/10.1016/j.lwt.2025.117453
• Akhtar, S., Rauf, A., Imran, M., Qamar, M., Riaz, M., & Mubarak, M.S. (2017). Black carrot (Daucus carota L.), dietary and health promoting perspectives of its polyphenols: A review. Trends in Food Science & Technology, 66, 36-47. https://doi.org/10.1016/j.tifs.2017.05.004
• Aktı, N., & Yıldız, S. (2025). Exploring ultrasound-induced changes on bioactive content and color properties of sour cherry juice involving insights from image processing analysis. Journal of Food Quality, 12. https://doi.org/10.1155/jfq/3204362
• Algarra, M., Fernandes, A., Mateus, N., Freitas, V., Esteves da Silva, J.C.G., & Casado, J. (2014). Anthocyanin profile and antioxidant capacity of black carrots (Daucus carota L. ssp. sativus var. atrorubens Alef.) from Cuevas Bajas, Spain. Journal of Food Composition and Analysis, 33 (1), 71-76. https://doi.org/10.1016/j.jfca.2013.11.005
• Apak, R., Güçlü, K., Özyürek, M., & Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52 (26), 7970-7981. https://doi.org/10.1021/jf048741x
• Aslam, R., Alam, M.S., Ali, A., Tao, Y., & Manickam, S. (2023). A chemometric approach to evaluate the effects of probe-type ultrasonication on the enzyme inactivation and quality attributes of fresh amla juice. Ultrasonics Sonochemistry, 92, 106268. https://doi.org/10.1016/j.ultsonch.2022.106268
• Bay Yılmaz, B., & Pekmez, H. (2020). Quality characteristics and antioxidant properties of bread incorporated by black carrot (Daucus carota ssp. sativus var. atrorubens Alef) fiber. Gıda, 45 (2), 290-298. https://doi.org/10.15237/gida.GD19134
• Berentzen E.I., Møller, A.H., Danielsen, M., Jensen, M., Joernsgaard, B., & Dalsgaard, T.K. (2024). Stability of individual anthocyanins from black carrots stored in light and darkness – Impact of acylation. Food Research International, 186, 114382. https://doi.org/10.1016/j.foodres.2024.114382
• Bhat, R., Kamaruddin, N.S.B.C., Min-Tze, L., & Karim, A.A. (2011). Sonication improves kasturi lime (Citrus microcarpa) juice quality. Ultrasonics Sonochemistry, 18 (6), 1295-1300. https://doi.org/10.1016/j.ultsonch.2011.04.002
• Cebeci, E., & Hancı, F. (2017). Mor havuçta (Daucus carota ssp. sativus var. atrorubens Alef) kendilemenin tohum verim ve kalitesine etkileri. Akademik Ziraat Dergisi, 6, 99-102.
• Cemeroğlu, B. (2010). Gıda analizleri. (Genişletilmiş 2. Baskı) Gıda Teknolojisi Derneği Yayınları, Bizim Grup Basımevi, Ankara.
• Chen, F., Sun, Y., Zhao, G., Liao, X., Hu, X., Wu, J., & Wang, Z. (2007) Optimization of ultrasound-assisted extraction of anthocyanins in red raspberries and identification of anthocyanins in extract using high-performance liquid chromatography–mass spectrometry. Ultrasonics Sonochemistry, 14, 767-778.
• Comarella, C.G., Treptow, T.C., de Oliveira, Á.S., Rodrigues, E., Sautter, C.K., Bochi, V., & Penna, N.G. (2022), Ultrasound irradiation of grapes: Effect on the anthocyanin profile of “Isabella” juice". British Food Journal, 124 (4), 1333-1349. https://doi.org/10.1108/BFJ-01-2021-0105
• de Souza Carvalho, L.M., Lemos, M.C.M., Sanches, E.A., da Silva, L. S., de Araújo Bezerra, J., Aguiar, J.P.L., das Chagas do Amaral Souza, F., Alves Filho, E.G., & Campelo, P.H. (2020). Improvement of the bioaccessibility of bioactive compounds from Amazon fruits treated using high energy ultrasound. Ultrasonics Sonochemistry, 67, 105148. https://doi.org/10.1016/J. ULTSONCH.2020.105148
• Dinçer, C., & Topuz, A. (2018). Meyve suyu işlemede ultrases kullanımı. Gıda, 43 (4), 569-581. https://doi.org/10.15237/gida.GD18037
• Ferreira, D.P., Magnani, M., Silva, F.A., de Oliveira, L.V.A., Rosset, M., Verruck, S., & Pimentel, T.C. (2023). Understanding the potential of ultrasound as an innovative tool for microbial inactivation, functionalization of plant-based foods, and improvements of functional food potential. Food Bioscience, 56, 103342. https://doi.org/10.1016/j.fbio.2023.103342
• Genç, N., & Bayan, Y. (2016). Salvia verticillata subsp. amasiaca’nın toplam fenolik madde ve antioksidan kapasitesinin belirlenmesi. Nevşehir Bilim ve Teknoloji Dergisi, 5 (2), 158-166. http://hdl.handle.net/20.500.11787/5656
• Guimaraes, J.T., Silva, E.K., Ranadheera, C.S., Moraes, J., Raices, R.S.L., Silva, M.C., Ferreira, M.S., Freitas, M.Q., Meireles, M.A.A., & Cruz, A.G. (2019). Effect of high-intensity ultrasound on the nutritional profile and volatile compounds of a prebiotic soursop whey beverage. Ultrasonics Sonochemistry, 55, 157-164. https://doi.org/10.1016/j.ultsonch.2019.02.025
• Gülçin, İ., Küfrevioğlu, Ö.İ., Oktay, M., & Büyükokuroğlu, M.E. (2004). Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). Journal of Ethnopharmacology, 90 (2-3), 205-215. https://doi.org/10.1016/j.jep.2003.09.028
• Güneş, G. (2008). Şalgam suyu üretiminde en uygun siyah havuç (Daucus carota) miktarının belirlenmesi üzerine bir araştırma. Yüksek Lisans Tezi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Gıda Mühendisliği Anabilim Dalı, Adana.
• Güneşer, O., & Erdoğan, S. (2023). Ultrases uygulamasının süt bileşenleri üzerine etkisi. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi, 7 (1), 40-58. https://doi.org/10.47137/usufedbid.1209666
• Güzel, N., & Bahçeci, K.S. (2019). Çorum yöresi ballarının fenolik madde içerikleri ile renk ve antioksidan kapasiteleri arasındaki ilişki. Gıda, 44 (6), 1148-1160. https://doi.org/10.15237/gida.GD19095
• Hasheminya, S.M., & Dehghannya, J. (2022). Non-thermal processing of black carrot juice using ultrasound: Intensification of bioactive compounds and microbiological quality. International Journal of Food Science and Technology, 57 (9), 5848-5858. https://doi.org/10.1111/ijfs.15901
• Hayatioğlu, N., Tekin, İ., & Ersus, S. (2024). Optimization of cellulose extraction parameters and production of nanocellulose from black carrot juice wastes. Tekirdağ Ziraat Fakültesi Dergisi, 21 (2), 547-560. https://doi.org/10.33462/jotaf.1326627
• Kaur, N., Aggarwal, P., A. P., & Sandhu, K.P. (2023). Influence of conventional blanching and sonication on the quality parameters of black carrot (Daucus carota L.) juice: a comparative analysis. International Journal of Food Science, 58, 5283-5290. https://doi.org/10.1007/s13197-010-0170-6
• Kemsawasd, V., & Chaikham, P. (2021). Alteration of bioactive compounds and antioxidative properties in thermal, ultra-high pressure and ultrasound treated maoberry (Antidesma bunius L.) juice during refrigerated storage. Current Research in Nutrition and Food Science Journal, 9 (3), 904-916. http://dx.doi.org/10.12944/CRNFSJ.9.3.17
• Lepaus, B.M., Valiati, B.S., Machado, B.G., Domingos, M.M., Silva, M.N., Faria-Silva, L., Bernardes, P.C., Oliveira, D.S., & de São José, J.F.B. (2023). Impact of ultrasound processing on the nutritional components of fruit and vegetable juices. Trends in Food Science and Technology, 138, 752-765. https://doi.org/10.1016/j.tifs.2023.07.002
• Manzoor, M.F., Xu, B., Khan, S., Shukat, R., Ahmad, N., Imran, M., Rehman, A., Karrar, E., Aadil, R.M., & Korma, S.A. (2021). Impact of high-intensity thermosonication treatment on spinach juice: Bioactive compounds, rheological, microbial, and enzymatic activities. Ultrasonics Sonochemistry, 78, 105740. https://doi.org/10.1016/j.ultsonch.2021.105740
• Menelli, G.S., Fracalossi, K.L., Lepaus, B.M., & De São José, J.F.B. (2021). Effects of high-intensity ultrasonic bath on the quality of strawberry juice. CyTA - Journal of Food, 19 (1), 501-510. https://doi.org/10.1080/19476337.2021.1918768
• Nadeem, M., Ubaid, N., Qureshi, T.M., Munira, M., & Mehmood, A. (2018). Effect of ultrasound and chemical treatment on total phenol, flavonoids and antioxidant properties on carrot-grape juice blend during storage. Ultrasonics Sonochemistry, 45, 1-6. https://doi.org/10.1016/j.ultsonch.2018.02.034
• Polat, S., Güçlü, G., Kelebek, H., Keskin, M., & Selli, S. (2022). Comparative elucidation of colour, volatile and phenolic profiles of black carrot (Daucus carota L.) pomace and powders prepared by five different drying methods. Food Chemistry, 369, 130941. https://doi.org/10.1016/j.foodchem.2021.130941
• Reche, C., Rosello, C., Eim, V., Leon, A.E., & Simal, S. (2023). High-power ultrasound in gas phase: Effects on the bioactive compounds release from red bell pepper during invitro gastrointestinal digestion. Antioxidants, 12, 356. https://doi.org/10.3390/antiox12020356
• Samarasinghe, H.G.A.S., Dharmaprema, S., Manodya, U., Kariyawasam, K.P., & Samaranayake, U.C. (2024). Exploring ımpact of the ultrasound and combined treatments on food quality: A comprehensive review. Turkish Journal of Agriculture-Food Science and Technology, 12 (2), 349-365.
• Singleton, V.L., Orthofer, R., & Lamuela-Raventós, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol, 299, 152-178.
• Starek, A., Kobus, Z., Sagan, A., Chudzik, B., Pawłat, J., Kwiatkowski, M., Terebun, P., & Andrejko, D. (2021). Influence of ultrasound on selected microorganisms, chemical and structural changes in fresh tomato juice. Scientifc Reports, 11 (1), 3488, 1-12. https://doi.org/10.1038/s41598-021-83073-8
• Tahmaz Karaman, H., Yüksel Küskü, D., Söylemezoğlu, G., & Çelik, H. (2022). Vitis labrusca L. genotiplerinin fenolik bileşik ve antioksidan kapasite içerikleri. Tekirdağ Ziraat Fakültesi Dergisi, 19 (2), 318-331. https://doi.org/10.33462/jotaf.952108
• Tiwari, B.K., Muthukumarappan, K., O’Donnell, C.P., & Cullen, P.J. (2008). Effects of sonication on the kinetics of orange juice quality parameters. Journal of Agricultural and Food Chemistry, 56 (7), 2423-2428. https://doi.org/10.1021/jf073503y
• Tiwari, B.K., O’Donnell, C.P., & Cullen, P.J. (2009). Effect of sonication on retention of anthocyanins in blackberry juice. Journal of Food Engineering, 93 (2), 166-171. https://doi.org/10.1016/j.jfoodeng.2009.01.027
• Vercet, P., Lopez, A., & Burgos, J. (1998). Free radical production by monothermosonication. Ultrasonics, 36, 615-618.
• Wang, J., Vanga, S.K., & Raghavan, V. (2019b). High-intensity ultrasound processing of kiwifruit juice: Effects on the ascorbic acid, total phenolics, flavonoids and antioxidant capacity. LWT - Food Science and Technology, 107, 299-307. https://doi.org/10.1016/j.lwt.2019.03.024
• Wang, J., Wang, J., Ye, J., Vanga, S.K., & Raghavan, V. (2019a). Influence of high intensity ultrasound on bioactive compounds of strawberry juice: Profiles of ascorbic acid, phenolics, antioxidant activity and microstructure. Food Control, 96, 128-136. https://doi.org/10.1016/j.foodcont.2018.09.007
• Shen, Y., Zhu, D., Xi, P., Cai,T., Cao, X., Liu, H., & Li, J. (2021). Effects of temperature-controlled ultrasound treatment on sensory prope rties, physical characteristics and antioxidant activity of cloudy apple juice. LWT - Food Science and Technology, 142, 111030. https://doi.org/10.1016/j.lwt.2021.111030
• Yıkmış, S., Türkol, M., Pacal, I., Altan, A.D., Tokatlı, N., Abdi, G., Tokatlı Demirok, N., & Aadil, R.M. (2025). Optimization of bioactive compounds and sensory quality in thermosonicated black carrot juice: A study using response surface methodology, gradient boosting, and fuzzy logic. Food Chemistry: X, 25, 102096. https://doi.org/10.1016/j.fochx.2024.102096
• Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64 (4), 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2