THREE NEW BIS(ACYLHYDRAZONE) MONONUCLEAR TRANSITION METAL COMPLEXES: SYNTHESIS, CHARACTERIZATIONS AND DNA INTERACTION STUDIES

Abstract

In recent years, studies on the design and synthesis of artificial nucleases with biological, structural and coordination properties have been increasing. With this study, mononuclear complex compounds were obtained with Cu(II), Ni(II) and Zn(II) transition metal ion salts of bis(acylhydrazone) ligand, which has the potential to show biological activity and has revolving atoms such as N and O in its structure. The characteristic structures of the obtained complex compounds were elucidated using various techniques such as FTIR, UV-Visible, 1H-NMR, TGA, elemental analysis and magnetic susceptibility. The DNA-binding activities of potential artificial metallonucleases, whose structures were elucidated, were investigated using the UV-visible absorption titration method. Additionally, their DNA-cleavge activities were analyzed using the agarose gel electrophoresis method. The DNA binding mode and cleavage mechanisms were determined as part of this study.. In line with the results obtained, it was determined that the binding mode between DNA and the complexes is a noncovalent interaction. It has been demonstrated that the compounds cleavage DNA both oxidatively and hydrolytically. It has been revealed that the radicals involved in DNA cleavage activity are H2O2, superoxide and hydroxyl radicals. It was revealed that the compound with the highest cleavage and bonding interaction was the Cu(II) complex, followed by Ni(II) and Zn(II) complexes.

Keywords

• bis(acylhydrazones)
• metal complexes
• DNA interactions

Supporting Institution

Tubitak

Project Number

112T315

ÜÇ YENİ BİS(AÇİLHİDRAZON) MONONÜKLEER GEÇIŞ METALI KOMPLEKSİ: SENTEZ, KARAKTERİZASYON VE DNA ETKİLEŞİM ÇALIŞMALARI

Abstract

Son yıllarda biyolojik açıdan aktif ve yapısında metal içeren yapay nükleazların tasarlanması ve sentezine yönelik çalışmalara giderek çoğalmaktadır. Gerçekleştirilen bu çalışma ile biyolojik aktivite gösterme potansiyeli olan ve yapısında N ve O gibi dönor atomlar bulunduran bis(açilhidrazon)ligandın Cu(II), Ni(II) ve Zn(II) geçiş metal iyon tuzlarıyla mononükleer kompleks bileşikleri elde edilmiştir. Elde edilen kompleks bileşiklerin karakteristik yapıları, FTIR, UV-Görünür, 1H-NMR, TGA, elemental analiz ve magnetic süssebilite gibi çeşitli teknikler kullanılarak aydınlatılmıştır. Yapıları aydınlatılan potansiyel yapay metallonükleazların UV-vis. adsorbsiyon titrasyon metodu ile DNA-bağlanma, agaroz jel elektroforez yöntemi ile de DNA-kesme aktiviteleri incelenmiştir. DNA bağlanma modu ve kesme mekanizmaları belirlenmiştir. Elde edilen sonuçlar doğrultusunda, DNA ile kompleksler arasındaki bağlanma modunun nonkovalent bir etkileşim olduğu belirlenmiştir. Bileşiklerin hem oksidadif hem de hidrolitik olarak DNA’ yı kestiği ortaya koyulmuştur. DNA kesme aktivitesinde rol alan radikallerin H2O2, superoksit ve hidroksil radikalleri olduğu ortaya çıkarılmıştır. Kesme ve bağlanma etkileşiminin en yüksek olduğu bileşiğin Cu(II) kompleksi olduğu ve bu sıralamayı Ni(II) ve Zn(II) komplekslerinin izlediği ortaya konmuştur.

Keywords

• bis(açilhidrazon)lar
• metal kompleksler
• DNA etkileşim

Project Number

112T315

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