COMPARISON OF THE MICROTITER PLATE METHOD AND THE CONGO RED AGAR TECHNIQUE IN THE DETERMINATION OF STAPHYLOCOCCAL BIOFILM

Abstract

Coagulase-Negative Staphylococci are opportunistic pathogens that are commensal in human flora. One of the most important virulence factors known in the pathogenesis of infections of these bacteria is biofilm formation. The Microtiter Plate Method and The Congo Red Agar Technique are widely used to reveal biofilm formation. This study aims to compare human coagulase negative Staphylococcus spp. bacterial isolates, biofilm formations with the Microtiter Plate Method and Congo Red Agar Technique. In the study, it was concluded that 35 of 41 human coagulase negative staphylococcal isolates did not form biofilms according to the microtiter plate method, 6 isolates formed a weak biofilm, and none of the isolates formed a biofilm on the Congo Red Agar surface. It has been concluded that the results of the Microtiter Plate Method are more reliable, since the interpretation of the result in the Congo Red Agar Technique is difficult and subjective, based on observation. Since there are very few studies in the literature comparing the biofilm formation of coagulase negative staphylococci with the Microtiter Plate Method and the Congo Red Agar Technique, this study will be among the preliminary studies and will contribute to the literature.

Keywords

• Biofilm
• Coagulase-Negative Staphylococci
• Congo Red Agar Technique
• Microtiter Plate Method
• Vagina

Supporting Institution

Anadolu University

Project Number

1710S548

Ethical Statement

Not applicable.

Thanks

This study was supported within the scope of the project numbered 1710S548 accepted by Anadolu University Scientific Research Projects Commission. The author(s) would like to thank Prof. Dr. Merih Kıvanç for her endless support.

STAFİLOKOK BİYOFİLMİNİN BELİRLENMESİNDE MİKROTİTRE PLAKA YÖNTEMİ VE KONGO KIRMIZISI AGAR TEKNİĞİ’NİN KARŞILAŞTIRILMASI

Abstract

Kolagülaz negatif stafiloloklar, insan florasında kommensal olarak yer alan fırsatçı patojenlerdir. Bu bakterilerin enfeksiyonlarının patogenezisinde bilinen en önemli virülans faktörlerinden biri biyofilm oluşumlarıdır. Biyofilm oluşumunun ortaya konmasında mikrotitre plaka yöntemi ve kongo kırmızısı agar tekniği yaygın bir şekilde kullanılmaktadır. Bu çalışmada, insan koagülaz negatif Staphylococcus spp. bakteri izolatlarının biyofilm oluşumlarının mikrotitre plaka yöntemi ve kongo kırmızısı agar tekniği ile karşılaştırılması amaçlanmıştır. Çalışmada, 41 insan koagülaz negatif stafilokok izolatından mikrotitre plaka yöntemine göre 35 tanesinin biyofilm oluşturmadığı, 6 izolatın zayıf derecede biyofilm oluşturduğu, kongo kırmızısı agar yüzeyinde ise hiçbir izolatın biyofilm oluşturmadığı sonucuna varılmıştır. Kongo kırmızısı agar tekniğinde sonucun yorumlanmasının güç ve gözleme dayalı olması sebebiyle, mikrotitre plaka yöntemi sonuçlarının daha güvenilir olduğu sonucuna varılmıştır. Literatürde koagülaz negatif stafilokokların biyofilm oluşumlarının mikrotitre plaka yöntemi ve kongo kırmızısı agar tekniğiyle karşılaştırıldığı neredeyse hiç çalışma olmaması sebebiyle, yapılan çalışma öncül çalışmalar arasında yer alıp, literatüre katkı sunacaktır.

Keywords

• Biyofilm
• Koagülaz Negatif Stafilokok
• Kongo Kırmızısı Agar Tekniği
• Mikrotitre Plaka Yöntemi
• Vajina

Project Number

1710S548

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