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Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi

2148-8118 2618-6020

Muğla Sıtkı Koçman Üniversitesi

Internal Diseases

İç Hastalıkları

Deneysel Kronik Pankreatitte N-Asetil Sistein Uygulamasinin Pankreatik Fibroz Üzerine Etkisi

The Effect of N-Acetylcysteine on Fibrosis in Experimental Rat Chronic Pancreatitis

https://orcid.org/0000-0002-9087-4806

Ocal

Ramazan

Health Sciences University, Gulhane Faculty of Medicine, Training and Research Hospital, Department of Hematology, Ankara

https://orcid.org/0000-0002-0936-2476

Tascı

İlker

Health Sciences University, Gulhane Faculty of Medicine, Department of Internal Medicine, Ankara

https://orcid.org/0000-0001-6234-3488

Devecı

Mehmet Salih

Health Sciences University, Gulhane Faculty of Medicine, Department of Pathology, Ankara

https://orcid.org/0000-0002-2148-5356

Comert

Bilgin

Dokuz Eylul University, Faculty of Medicine, Department of Intensive Care, Izmir

https://orcid.org/0000-0001-5867-6503

Isık

Ahmet Turan

Dokuz Eylul University, Faculty of Medicine, Department of Geriatrics, Izmir

https://orcid.org/0000-0003-4874-9603

Mas

Mehmet Refik

Dokuz Eylul University, Faculty of Medicine, Department of Geriatrics, Izmir

03 20 2019

6 1 26 32 12 07 2018 01 16 2019

2014

Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi

Bu çalışmada,N-asetilsisteinin, kronik pankreatit hayvan modelinde fibrozis, atrofi veoksidatif stres üzerindeki etkisini değerlendirmeyi amaçladık. Kronikpankreatit, ratların ana pankreatik kanallarına 0.4 ml, %2’lik trinitrobenzensülfanik asit (TNBS) enjeksiyonu ile uyarılır. 60 Sprague-Dawley sıçanı dörtgruba randomize edildi; Grup I (n= 15): salin (sham), Grup II (n= 15): fibrozis+ N-asetilsistein (NAC), Grup III (n= 15): fibrozis (TNBS), Grup IV: etanol. 8.haftanın sonunda tüm ratlar sakrefiye edildi ve pankreatik dokular, oksidatifstres belirteçleri, fibrozis ve atrofi progresyonları incelendi. NAC grubundadoku malondialdehid (MDA) düzeyleri anlamlı olarak düşük bulunurken (p <0.001);süperoksit dismutaz (SOD) ve glutation peroksidaz (GSH-Px) aktiviteleri TNBSgrubuna göre anlamlı olarak yüksek bulundu (sırasıyla p <0.001 ve p <0.001).Dokulardaki histopatolojik incelemede NAC grubunun histopatolojik skorunun TNBSgrubundan anlamlı derecede düşük olduğu izlenirken (p<0.003) oksidatif stresile histopatolojik skordaki artış arasında anlamlı bir ilişki olduğu saptandı(histopatolojik skor/MDA: p<0.03, SOD: p<0.04, GSH-Px: p<0.02). Sonuçolarak, NAC uygulaması kronik pankreatitte oksidatif stresi azaltmakta olupfibrozis ve atrofi üzerinde yararlı etkilere sahiptir.

In this study, weaimed to assess the effect of N-acetylcysteine on fibrosis, atrophy andoxidative stress with chronic pancreatitis in an animal model. Chronicpancreatitis is stimulated by injection of 0.4 ml, 2% trinitrobenzene sulfanicacid (TNBS) into major pancreatic ducts of the rats. 60 Sprague-Dawley ratswere randomized into four group; Group I (n=15): saline (control), Grup II(n=15): fibrosis + N-acetylcysteine (NAC), Grup III (n=15): fibrosis (TNBS),Grup IV: ethanol. At the end of the 8th week, all rats were sacrificed, and ratpancreatic tissues, oxidative stress markers and progression of fibrosis andatrophy were examined. superoxide dismutase (SOD) and glutation peroxidase(GSH-Px) activities were significantly higher with respect to TNBS group(respectively p<0.001 and p<0.001); whereas tissue malondialdehyde (MDA)levels were found significantly lower in NAC group (p<0.001). Histopathological analysis of the tissuesrevealed that histopathological score of NAC group was significantly lower thanTNBS group (p<0.003), and was found a significant relation between increasein oxidative stress and histopathological score (histopathological score/MDA:p<0.03, SOD: p<0.04, GSH-Px: p<0,02). In conclusion, administration ofNAC decreases oxidative stress in chronic pancreatitis and has beneficialeffects on fibrosis and atrophy.

Animal Model Experimental Chronic Pancreatitis Fibrosis N-Acetylcysteine

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