Assessing DNA Barcodes for Identification of Pufferfish Species (Tetraodontidae) in Turkish Marine Waters

Year 2017, , 55 - 66, 19.12.2017

Cemal Turan , Mevlüt Gürlek Deniz Ergüden Ali Uyan Serpil Karan Servet Ahmet Doğdu

https://doi.org/10.28978/nesciences.369538

Cited By: 9

Abstract

In Turkish marine waters, pufferfish belongs to Tetraondontidae family are represented with 8
species, Lagocephalus lagocephalus, L. sceleratus, L. spadiceus, L. suezensis, L. guentheri,
Sphoeroides pachygaster, Torquigener flavimaculosus and Tylerius spinosissimus. DNA
barcoding can be useful in the assessment of cryptic or morphologically similar species of
identification which is widespread in marine environment. DNA barcode identification of the eight
puffer species of the Tetraodontidae family in Turkish marine waters were examined by using
mtDNA sequencing of the amplified partial mitochondrial cytochrome c oxidase I (COI) gene. COI
contained 189 variable and 337 conservative nucleotides of which 183 were parsimony informative
over 526 bp. Mean genetic diversity all species was found 0.18164. The highest and lowest
nucleotide divergence was observed L.spadiceus (0.0022) and between L. sceleratus, L. suezensis,
L. lagocephalus, L. guentheri and S. pachygaster (0.0000) respectively. The number of detected
different haplotypes were 10 out of 23 sequences, and haplotype diversity was found to be 1.000. 

Keywords

Pufferfish, catch amount, Aegean Sea, Mediterranean Sea, Turkey

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