neu fen muh bil der

Necmettin Erbakan University Journal of Science and Engineering

2667-7989

Necmettin Erbakan Üniversitesi

10.47112/neufmbd.2025.99

Animal Cell and Molecular Biology

Hayvan Hücresi ve Moleküler Biyoloji

Oleandrin Downregulates Toll-Like Receptor Pathway Genes in Human Endometrial Carcinoma Cells

Oleandrin, İnsan Endometriyal Karsinom Hücrelerinde Toll Benzeri Reseptör Yolağı Genlerinin İfadesini Baskılar

https://orcid.org/0000-0002-5559-3910

Akçaalan

Sedef

NECMETTİN ERBAKAN ÜNİVERSİTESİ

https://orcid.org/0000-0002-3796-575X

Eroğlu Güneş

Canan

NECMETTIN ERBAKAN UNIVERSITY

https://orcid.org/0000-0001-5619-8958

Seçer Çelik

Fatma

ANKARA MEDİPOL UNIVERSITY

https://orcid.org/0000-0002-9234-1560

Kurar

Ercan

NECMETTIN ERBAKAN UNIVERSITY

12 31 2025

7 3 372 381 03 05 2025 05 29 2025

2019

Necmettin Erbakan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi

Endometrial cancer arises from the development of malignant cells in endometrial tissues and ranks as the sixth most prevalent neoplasm among women globally. Toll-like receptors (TLRs), as part of the innate immune system, have a key function in the detection of pathogens. Previous research has previously indicated an association between TLRs and different types of cancers, highlighting the role of TLR expression in cancer cells in relation to progression, apoptosis, and survival. Nerium oleander L., a member of the Apocynaceae family, has been traditionally employed in addressing various diseases. Oleandrin, a compound obtained from the leaves and seeds of N. oleander, has been shown to suppress the growth of various human cancer cells. This study aimed to examine the impact of oleandrin on TLR pathway genes in Ishikawa human endometrial carcinoma cells. Following oleandrin treatment, XTT proliferation assay was conducted at the 24., 48. and 72. hours. The IC50 value was found to be 75.3 nM at the 48 hour. The impact of oleandrin on the expression levels of TLRs (1-10) and MYD88 genes in Ishikawa cells was analyzed using qPCR. Oleandrin administration caused a notable decrease in the expression levels of TLR1, TLR6 and MYD88, whereas the expression of other TLRs remained relatively stable. In conclusion, oleandrin exhibited cytotoxic effects, significantly decreasing the viability of Ishikawa cancer cells and influencing the TLR signaling cascade through modulation of the levels of TLR1, TLR6, and MYD88 genes.

Endometrial kanser, endometriyal dokularda kötü huylu hücrelerin gelişiminden kaynaklanır ve dünya genelinde kadınlar arasında altıncı en yaygın kanser türüdür. Doğuştan gelen bağışıklık sisteminin bir bileşeni olan Toll benzeri reseptörler (TLR’ler), patojenlerin tanınmasında kritik bir işlev üstlenir. Önceki araştırmalar, TLR’ler ile farklı kanser türleri arasında bir ilişki olduğunu ve TLR ekspresyonunun kanser hücrelerinde progresyon, apoptoz ve hayatta kalma ile ilişkili olduğunu göstermiştir. Apocynaceae familyasından olan Nerium oleander L., geleneksel olarak çeşitli hastalıkların tedavisinde kullanılmıştır. N. oleander’in yaprakları ve tohumlarından elde edilen oleandrin bileşiği, çeşitli insan kanser hücrelerinin büyümesini baskıladığı gösterilmiştir. Bu çalışma, oleandrinin Ishikawa insan endometrial karsinom hücrelerinde TLR yolağı genleri üzerine etkisini incelemeyi amaçlamıştır. Oleandrin uygulamasının ardından, XTT proliferasyon testi 24., 48. ve 72. saatlerde gerçekleştirilmiştir. IC50 değeri 48. saat için 75,3 nM olarak belirlenmiştir. Oleandrinin TLR’ler (1-10) ve MYD88 genlerinin ekspresyon seviyeleri üzerindeki etkisi qPCR ile analiz edilmiştir. Oleandrin uygulaması sonucunda TLR1, TLR6 ve MYD88 genlerinin ekspresyon seviyelerinde belirgin bir azalma ile sonuçlanırken, diğer TLR’lerin ifadesi nispeten stabil kalmıştır. Sonuç olarak, oleandrin, Ishikawa kanser hücrelerinin canlılığını önemli ölçüde azaltarak, TLR1, TLR6 ve MYD88 genlerinin seviyelerinin modülasyonu yoluyla TLR sinyal yolaklarını etkileyen sitotoksik etkiler göstermiştir.

Endometrial carcinoma Oleandrin Toll like receptors

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