Yıl 2020, Cilt 1 , Sayı 2, Sayfalar 53 - 58 2020-09-30

In the current work, the effects of curcumin on testicular tissues in Ehrlich Ascites Tumor (EAT) model developed in Balb/C mice. EAT cells (1x106) received from stock of animals were injected intraperitoneally to animals. 25mg/kg and 50mg/kg of curcumin was administered intraperitoneally. Testicular tissues obtained after all the experiment were evaluated for histopathological and biochemical parameters. Histopathological results showed that 50 mg/kg curcumin group had less EAT cells around testicular tissues than tumor control group. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities and reduced glutathione (GSH), oxide glutathione (GSSG), total antioxidant status (TAS), total oxidant status (TOS), interleukin-1beta (IL-1β), interleukin-6, interleukin-17, tumor necrosis factor-alfa (TNF-α) levels were measured in testis tissues. Oxidative stress index (OSI) and GSH/GSSG ratio were calculated. Findings clearly suggested that antioxidant parameters (except CAT and GPx) had higher value in animal models treated with 25 and 50 mg curcumin groups associated to tumor control group. There was a statistically noteworthy variance between the groups in all parameters. Antitumor effect of curcumin on ascites tumor cells produced by EAT cells evidenced with histopathological while antioxidant and anti-inflammatory biochemical parameters evidenced with biochemical parameters.
Testis, EAT, Mice, Curcumin, Antioxidant
  • Referans1 Kayaalp SO. Tıbbi Farmakoloji, Hacettepe Taş Kitapçılık. Ankara. 1996, pp. 731-79.
  • Referans2 Weiss RB, Henney JE, DeVita VT. Multimodal treatment of primary breast carcinoma. analiysis of accomplishments and problem areas. The Amercian Journal of Medicine. 1981; 70:844-851.
  • Referans3 Pei Y, Ai T, Deng Z, et al. Impact of plant extract on the gastrointestinal fate of nutraceutical-loaded nanoemulsions: phytic acid inhibits lipid digestion but enhances curcumin bioaccessibility. Food Funct. 2019;106: 3344-3355.
  • Referans4 Toraya S, Uehara O, Daichi H, et al. Curcumin inhibits the expression of proinfammatory mediators and MMP 9 in gingival epithelial cells stimulated for a prolonged period with lipopolysaccharides derived from Porphyromonas gingivalis. Odontology. 2020; 108(1); 16-24.
  • Referans5 Bhaumik S, Anjum R, Rangaraj N, et al. Curcumin mediated apoptosis in AK-5 tumor cells involves the production of reactive oxygen intermediates. FEBS Lett. 1999; 456:311-314.
  • Referans6 Basham SA, Hunter S. W, Ben M. K, et al. Effect of Curcumin Supplementation on ExerciseInduced Oxidative Stress, Inflammation, Muscle Damage, and Muscle Soreness. Journal of Dietary Supplements. 2019; 26;1-14.
  • Referans7 Kocaadam B, Şanlıer N. Curcumin, an active component of turmeric (curcuma longa), and Its effects on health. Crit Rev Food Sci Nutr. 2015; 3:123-127.
  • Referans8 Hatcher H, Planalp R, Cho J, et al. Curcumin from ancient medicie to current clinical trials. Cell Moll Life. 2008; 65:1631-1652.
  • Referans9 Gevorkyan L, Gambashidze K. Anticancer efficacy of hydroxyethylthiamine diphosphate in vivo. Exp Oncol. 2014; 36:48-49.
  • Referans10 Birben E, Sahiner UM, Sackesen C, et al. Oxidative Stress and Antioxidant Defense. WAO Journal. 2012;5:9–19.
  • Referans11 Limon-Pacheco J, Gonsebatt ME. The role of antioxidants and antioxidant related enzymes in protective responses to environmentally induced oxidative stress. Mutat Res. 2009; 674(1-2):137-147.
  • Referans12 Young IS, Woodside JV. Antioxidants in Health and Disease. J Clin Pathol. 2001; 54(3):176-186.
  • Referans13 Reiter RJ, Melchiorri D, Sewerynek E, et al. A review of the evidence supporting melatonin‘s role as an antioxidant. J Pineal Res. 1995; 18(1):1-11.
  • Referans14 Gürdöl F. Tıbbi biyokimya. 3. Baskı. 2018, pp. 644.
  • Referans15 Joanisse DR, Storey KB. Oxidative damage and antioxidants in Rana syvatica, the freeze-tolerant wood frog. Am. J. Physiol. 1996; 271:545 – 553.
  • Referans16 Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 2004; 37:277–285.
  • Referans17 Mohan ML, Vasudevan NT, Naga Prasad SV. Proinflammatory Cytokines Mediate GPCR Dysfunction. J Cardiovasc Pharmacol. 2017; 70(2):61-73.
  • Referans18 Oztürk O, Gümüşlü S. Changes in glucose-6-phosphate dehydrogenase, copper, zinc-superoxide dismutase and catalase activities, glutathione and its metabolizing enzymes, and lipid peroxidation in rat erythrocytes with age. Exp Gerontol. 2004; 39(2):211-6.
  • Referans19 Scott MS, William T. Epidemiology and Diagnosis of Testis Cancer. Urologic Clinics of North America. 2015; 42(3):269-275.
  • Referans20 Raju A, Christina MJA, Murali A. Antitumor activity of ethanol and aqueous extracts of Drosera Burmannii vahl. İn EAC Bearing Mice. Spatula DD. 2012; 2:83-88.
  • Referans21 Azab ME, Hishe H, Moustapha Y, et al. Anti-angiogenic effect of resveratrol or curcumin in ehrlich ascites carcinoma-bearing mice. Eur J Pharmacol. 2011; 652:7-14.
  • Referans22 Maheshwari RK, Singh AK, Gaddipati J, et al. Multiple biological activities of curcumin: A short review. Life science. 2006; 78:2081-2087.
  • Referans23 Kohli K, Ali J, Ansari MJ, et al. Curcumin a antiinflammatory agent. Indian J Pharmacol. 2005; 37:141-147.
  • Referans24 Chainoglou E, Hadjipavlou-Litina D. Curcumin analogues and derivatives with anti-proliferative and anti-inflammatory activity: Structural characteristics and molecular targets. Expert Opin Drug Discov. 2019; 16:1-22.
  • Referans25 Kanter P, Tarladaçalışır YT, Akpolat M, et al. Gamma radyasyona bağlı oluşan jejenum mukoza hasarına karşı curcumin ve amifostinin koruyucu etkilerinin incelenmesi. Tıp araştırmaları dergisi. 2008; 6(3):128-135.
  • Referans26 Lopez LM. Anticancer and carcinogenic properties of curcumin. Considerations for its clinical development as a cancer chemopreventive and chemotherapeutic agent. Mol. Nutr. Food Res. 2008; 52:103–127.
  • Referans27 Pan HM, Huang TM, Lın JK. Biotransformation of curcumin through reduction and glucuronidation in mice. The American Society for Pharmacology and Experimental Therapeutics. 1998; 27:486-493.
  • Referans28 Facchini JM, Alves EP, Aguilera C, et al. Antitumor activity of pleurotus ostreatus polysaccharide fractions on ehrlich tumor and sarcoma. International Journal of Biological Macromolecules. 2014; 68:72-77.
  • Referans29 Yılmaz S, Ülger H, Ertekin T, et al. Investigating the anti-tumoral effect of curcumin on the mice in which Ehrlich ascites and solid tumor is created. Iran J Basic Med Sci. 2019; 22:418-425.
  • Referans30 Yılmaz S, Alpa Ş, Nisari M, et al. Examining the Antitumoral Effect of Cornelian Cherry (Cornus mas) in Ehrlich Ascites Tumor‑induced Mice. J Anat Soc India. 2019; 68:16-22. Referans31 Yang W, Zhang J, Zhang B. The role of ketanserin in maintaining cırculatıon stability in endotoxic shock rats. Acta Medica Mediterranea. 2019; 35: 2871.
  • Referans32 Ueki M, Ueno M, Morishita J, et al. Curcumin ameliorates cisplatin-induced nephrotoxicity by inhibiting renal inflammation in mice. Journal of Bioscience and Bioengineering. 2013; 115:547-551.
  • Referans33 Cho JW, Lee KS, Kim CW. Curcumin attenuates the expression of IL-1beta, IL-6, and TNF-alpha as well as cyclin E in TNF-alpha-treated HaCaT cells; NF-kappaB and MAPKs as potential upstream targets. Int J Mol Med. 2007; 19(3):469-74.
  • Referans34 Bhaumik S, Jyothi MD, Khar A. Differential modulation of nitric oxide production by curcumin in host macrophages and NK cells. FEBS Lett. 2000; 483:78–82.
  • Referans35 Brouetand I, Ohshima H. Curcumin, an anti-tumour promoter and anti-inflammatory agent, inhibits induction of nitric oxide synthase in activated macrophages. Biochem Biophys Res Commun. 1995; 206:533–540.
  • Referans36 Khopde M S, Priyadarsini KI, Venkatesan P, et al. Free radical scavenging ability and antioxidant efficiency of curcumin and its substituted analogue. Biophys Chem. 1999; 80(2):85-91.
  • Referans37 Venkatesan N, Punithavathi V, Chandrakasan G. Curcumın protects bleomycın-ınduced lung ınjury ın rats. Life Seienm. 1997; (61):5l-58.
  • Referans38 Kuhad A, Chopra K. Curcumin attenuates diabetic encephalopathy in rats: behavioral and biochemical evidences. Eur J Pharmacol. 2008; 576:34–42.
  • Referans39 Sahin E, Gümüşlü S. Immobilization stress in rat tissues: alterations in protein oxidation, lipid peroxidation and antioxidant defense system. Comp Biochem Physiol C Toxicol Pharmacol. 2007; 144(4):342-7.
Birincil Dil en
Konular Androloji
Bölüm Research Articles
Yazarlar

Yazar: Seher YİLMAZ (Sorumlu Yazar)
Kurum: BOZOK ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Yazar: Yeşim GÖÇMEN
Kurum: YOZGAT BOZOK ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Yazar: Adem TOKPINAR
Kurum: YOZGAT BOZOK ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Yazar: Şükrü ATEŞ
Kurum: YOZGAT BOZOK ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Yazar: Mustafa NİSARİ
Kurum: NUH NACİ YAZGAN ÜNİVERSİTESİ
Ülke: Turkey


Yazar: Aysen CANİKLİOĞLU
Kurum: YOZGAT BOZOK ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0001-9568-9442
Yazar: Ünal ÖZTEKİN
Kurum: YOZGAT BOZOK ÜNİVERSİTESİ, TIP FAKÜLTESİ, TIP PR.
Ülke: Turkey


Tarihler

Başvuru Tarihi : 3 Temmuz 2020
Kabul Tarihi : 19 Ağustos 2020
Yayımlanma Tarihi : 30 Eylül 2020

EndNote %0 New Trends in Medicine Sciences Effect of Curcumin on Testis in Mice with Ehrlich Ascites Tumor %A Seher Yilmaz , Yeşim Göçmen , Adem Tokpınar , Şükrü Ateş , Mustafa Nisari , Aysen Caniklioğlu , Ünal Öztekin %T Effect of Curcumin on Testis in Mice with Ehrlich Ascites Tumor %D 2020 %J New Trends in Medicine Sciences %P 2717-8161-2717-8161 %V 1 %N 2 %R %U