ALDH Expression in Hematopoietic Stem Cells Derived From Cord Blood: Effect of Transfer Time.

Yıl 2021, Cilt: 2 Sayı: 2, 111 - 115, 15.09.2021

Durmus Burgucu

Öz

The human aldehyde dehydrogenase (ALDH) activity measurements have recently been considered a quick and accurate quality control test used to determine the function of cord blood cells. The relationship between high ALDH activity of hematopoietic stem cells and engraftment is also known. However, there is limited data on the relationship between ALDH expression and transfer time of the cord blood to the laboratory in cord blood banking. The aim of this study is to investigate whether the transfer time has an effect on ALDH expression. 20 volunteers were included in the study. After collection of the cord blood, transfer times to the laboratory were calculated. Subsequently, CD34+ cell count, Total Nucleated Cell (TNC) count, and ALDH expression were analyzed. ALDH expression was found to be high in cord blood containing a high number of CD34+ cells. Similarly, a positive correlation was detected between TNC count and ALDH expression. There was no correlation between the transfer time, which is an important parameter in cord blood banking, and ALDH expression. The findings of the present study show that ALDH test can be used in cord blood banking, and it reveals for the first time that it can be used safely regardless of the transfer time.

Anahtar Kelimeler

HEMATOPOIETIC STEM CELLS, ALDH, CORD BLOOD

Kaynakça

• [1] Marchitti SA, Brocker C, Stagos D, Vasiliou, V. Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily. Expert Opin Drug Metab Toxicol 2008;4:697-720.
• [2] Black WJ, Stagos D, Marchitti SA, Nebert DW, Tipton KF, Bairoch A, Vasiliou V. Human aldehyde dehydrogenase genes: alternatively spliced transcriptional variants and their suggested nomenclature. Pharmacogenet Genomics 2009;19:893-902.
• [3] Sladek NE. Human aldehyde dehydrogenases: potential pathological, pharmacological, and toxicological impact. J Biochemic Mol Toxic 2003;17:7-23.
• [4] Kastan MB, Schlaffer E, Russo JE, Colvin OM, Civin CI., Hilton J. Direct demonstration of elevated aldehyde dehydrogenase in human hematopoietic progenitor cells. Blood 1990;75: 1947-1950.
• [5] Corti S, Locatelli F, Papadimitriou D, et al. Identification of a primitive brain-derived neural stem cell population based on aldehyde dehydrogenase activity. Stem Cells 2006;24:975-985
• [6] Jean E, Laoudj-Chenivesse D, Notarnicola C, et al. Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. J Cell Mol Med. 2011;15(1):119-133.
• [7] Reya T, Morrison SJ, Clarke MF, Weissman IL. Stem cells, cancer, and cancer stem cells. Nature 2001;414:105-111.
• [8] Ma I, Allan AL. The Role of Human Aldehyde Dehydrogenase in Normal and Cancer Stem Cells. Stem Cell Rev and Rep 2011;7:292-306
• [9] Huang X, Guo B, Capitano M, Broxmeyer HE. Past, present, and future efforts to enhance the efficacy of cord blood hematopoietic cell transplantation. F1000Res. 2019;8:F1000 Faculty Rev-1833. Published 2019 Oct 31
• [10] Clark P, Trickett A, Stark D, Vowels M. Factors affecting microbial contamination rate of cord blood collected for transplantation. Transfusion 2012;52(8):1770-1777.
• [11] Tanaçan A, Yurdakul P, Aktoz F, Örgül G, Beksaç M, Beksaç MS. Factors influencing the success of cord blood collection: a tertiary perinatal medicine center’s experience. Turk J Med Sci 2018;48(5):961-966.
• [12] Pasha R, Howell A, Turner TR, et al. Transient warming affects potency of cryopreserved cord blood units [published online ahead of print, 2020 Jun 24]. Cytotherapy 2020;S1465-3249(20)30566-1
• [13] Frandberg S, Borestrom C, Li S, Fogelstrand L, Palmqvist L. Exploring the heterogeneity of the hematopoietic stem and progenitor cell pool in cord blood: simultaneous staining for side population, aldehyde dehydrogenase activity, and CD34 expression. Transfusion 2015;55:1283-1289.
• [14] Lee HR, Shin S, Yoon JH, Roh EY, Kim BJ, Song EY. Aldehyde dehydrogenase-bright cells correlated with the colony-forming unit-granulocyte-macrophage assay of thawed cord blood units. Transfusion 2014;54:1871-1875.
• [15] Vassalli G. Aldehyde Dehydrogenases: Not Just Markers, but Functional Regulators of Stem Cells. Stem Cells Int. 2019;2019:3904645.
• [16] Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother 1996;5(3):213-226.
• [17] Jordan CT, Lemischka IR. Clonal and systemic analysis of long-term hematopoiesis in the mouse. Genes Dev 1990;4:220-232.
• [18] Akashi K, Traver D, Miyamoto T, Weissman IL. A clonogenic common myeloid progenitor that gives rise to all myeloid lineages. Nature 2000;404:193-197.
• [19] Bensinger WI, Buckner CD, Shannon-Dorcy K, et al. Transplantation of allogeneic CD34+ peripheral blood stem cells in patients with advanced hematologic malignancy. Blood 1996;88:4132-4138.
• [20] Frändberg S, Li S, Boreström C, Holgersson J, Palmqvist L. The aldehyde dehydrogenase cord blood potency assay excludes early apoptotic cells. Transfusion 2018;58(6):1452-1457.
• [21] Hussein E, DeFor T, Wagner JE, Sumstad D, Brunstein CG, McKenna DH. Evaluation of post-thaw CFU-GM: clinical utility and role in quality assessment of umbilical cord blood in patients receiving single unit transplant. Transfusion 2020;60(1):144-154.
• [22] Shoulars K, Noldner P, Troy JD, et al. Development and validation of a rapid, aldehyde dehydrogenase bright-based cord blood potency assay. Blood 2016;127:2346-54
• [23] Gençer EB, Yurdakul P, Dalva K, Beksaç M. Flow Cytometric Aldehyde Dehydrogenase Assay Enables a Fast and Accurate Human Umbilical Cord Blood Hematopoietic Stem Cell Assessment. Turk J Haematol 2017;34(4):314-320.