Potential Beneficial Effects of Apelin-13 on Testicular Ischemia/Reperfusion Injury

Yıl 2024, Cilt: 5 Sayı: 2, 98 - 103, 30.05.2024

Ayhan Tanyeli , Mustafa Can Güler , Ersen Eraslan , Saime Özbek Şebin , Burak Bircan , Engin Şebin , Fazile Nur Ekinci Akdemir

https://doi.org/10.56766/ntms.1476451

Öz

Testicular ischemia-reperfusion (T I/R) injury leads to oxidative stress with excessive accumulation of reactive oxygen species in the tissue. This phenomenon has an important place in the pathophysiology of testicular torsion injury. In the presented study, it was aimed to reveal the prophylactic beneficial effects of apelin 13 (APE-13 ) on T I/R damage. Twenty-four male Sprague Dawley rats were randomly divided into four groups as sham, I/R, 10µg/kg APE-13 and 100µg/kg groups. I/R protocol and apelin 13 application doses were applied with reference to previous studies. At the end of the experiment, all rats were sacrificed and their testicular tissues were quickly removed. It was stored under appropriate conditions until biochemical analysis was performed. In the biochemical analysis of the tissues, it was determined that oxidative parameters and inflammatory cytokine levels increased and antioxidant levels decreased in the testicular tissue due to I/R. On the other hand, it was observed that these results changed significantly in the 10µg/kg APE-13 and 100µg/kg groups. Considering the presented data, it was revealed that the severity of T I/R-induced tissue damage was reduced when APE-13 was administered at doses of 10µg/kg and 100µg/kg.

Anahtar Kelimeler

Apelin-13, Ischemia/Reperfusion, Testis

Etik Beyan

Ethical permissions for the study were obtained from Atatürk University Animal Experiments Local Ethics Committee (Date and number 30.03.2018/54).

Destekleyen Kurum

Atatürk University

Proje Numarası

TSA-2018-6719.

Teşekkür

None

Kaynakça

• Filho DW, Torres MA, Bordin AL, CrezcynskiPasa TB, Boveris A. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury. Mol Aspects Med. 2004; 25(1-2):199-210.
• Topdağı Ö, Tanyeli A, Ekinci Akdemir FN, Güzel Erdoğan D, Güler MC, E. E. Higenamine decreases testicular damage injured by ischemia reperfusion: a biochemical study. Turk J Sci. 2019;4:92-99.
• Fehér AM, Bajory Z. A review of main controversial aspects of acute testicular torsion. J Acute Dis. 2016; 5(1):1-8.
• Ruiz-Meana M, Garcia-Dorado D. Translational cardiovascular medicine (II). Pathophysiology of ischemia-reperfusion injury: new therapeutic options for acute myocardial infarction. Rev Esp Cardiol. 2009; 62(2):199-209.
• Tanyeli A, Ekinci Akdemir FN. The effect of Fraxin against lung and testis damage induced by testicular torsion/detorsion in rats. Ann Med Res. 2020; 27(10):2769-74.
• Granger DN. Role of Xanthine-Oxidase and Granulocytes in Ischemia-Reperfusion Injury. Am J Physiol. 1988; 255(6):H1269-H75.
• Al-Maghrebi M, Renno WM. Genistein alleviates testicular ischemia and reperfusion injury-induced spermatogenic damage and oxidative stress by suppressing abnormal testicular matrix metalloproteinase system the Notch 2/Jagged 1/Hes-1 and Caspase-8 Pathways. J Physiol Pharmacol. 2016; 67(1):129-37.
• Tanyeli A, Eraslan E, Ekinci Akdemir FN, Guler M, Güzel Erdogan D, Polat E, et al. Cryptotanshinone mitigates ischemia reperfusioninduced testicular damage: A experimental study. Ann Med Res. 2019; 26(11):2549-52.
• Tanyeli A, Eraslan E, Güler MC, Ekinci Akdemir FN, Güzel Erdoğan D, Topdagi O, et al. Investigation of the Effects of Maresin-1 on Testicular Ischemia Reperfusion Induced Oxidative Stress. South Clin Ist Eur. 2020; 31(3):187-91.
• Odowd BF, Heiber M, Chan A, Heng HHQ, Tsui LC, Kennedy JL, et al. A Human Gene That Shows Identity with the Gene Encoding the Angiotensin Receptor Is Located on Chromosome-11. Gene. 1993; 136(1-2):355-60.
• Lee HJ, Tomioka M, Takaki Y, Masumoto H, Saido TC. Molecular cloning and expression of aminopeptidase A isoforms from rat hippocampus. Bba-Gene Struct Expr. 2000; 1493(1-2):273-78.
• Masri B, Knibiehler B, Audigier Y. Apelin signalling: a promising pathway from cloning to pharmacology. Cell Signal. 2005; 17(4):415-26.
• Hatzelmann T, Harden LM, Roth J, Gerstberger R. Antipyretic effect of central [Pyr]apelin13 on LPS-induced fever in the rat. Regul Peptides. 2013; 184:6-13.
• Hessari FA, Sharifi M, Yousefifard M, Gholamzadeh R, Nazarinia D, Aboutaleb N. Apelin-13 attenuates cerebral ischemia/reperfusion injury through regulating inflammation and targeting the JAK2/STAT3 signaling pathway. J Chem Neuroanat. 2022; 126:102171.
• Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of Cutaneous Inflammation - Estimation of Neutrophil Content with an Enzyme Marker. J Invest Dermatol. 1982; 78(3):206-209.
• Ohkawa H, Ohishi N, Yagi K. Assay for Lipid Peroxides in Animal-Tissues by Thiobarbituric Acid Reaction. Anal Biochem. 1979; 95(2):351-58.
• Sun Y, Oberley LW, Li Y. A Simple Method for Clinical Assay of Superoxide-Dismutase. Clin Chem. 1988; 34(3):497-500.
• Arena S, Iacona R, Antonuccio P, Russo T, Salvo V, Gitto E, et al. Medical perspective in testicular ischemia-reperfusion injury. Exp Ther Med. 2017; 13(5):2115-22.
• Gülçin I. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012; 86(3):345-91.
• Ak T, Gülçin I. Antioxidant and radical scavenging properties of curcumin. Chem-Biol Interact. 2008; 174(1):27-37.
• Akhigbe RE, Ajayi LO, Adelakun AA, Olorunnisola OS, Ajayi AF. Codeine-induced hepatic injury is via oxido-inflammatory damage and caspase-3-mediated apoptosis. Mol Biol Rep.2020; 47(12):9521-30.
• Akhigbe RE, Odetayo AF, Akhigbe TMH, M. A. , Ashonibare PJ. Pathophysiology and management of testicular ischemia/reperfusion injury: Lessons from animal models. Heliyon. 2024; 10(9):e27760.
• Wu HH, Huang CC, Chang CP, Lin MT, Niu KC, Tian YF. Heat Shock Protein 70 (HSP70) Reduces Hepatic Inflammatory and Oxidative Damage in a Rat Model of Liver Ischemia/Reperfusion Injury with Hyperbaric Oxygen Preconditioning. Med Sci Monit. 2018; 24:8096-104.
• Ganjiani V, Bigham-Sadegh A, Ahmadi N, Divar MR, Meimandi-Parizi A, Asude M. The potential prophylactic and therapeutic impacts of niacin on ischemia/reperfusion injury of testis. J Pediatr Urol. 2024; 20(2):281 e1-e7.
• Shao ZQ, Dou SS, Zhu JG, Wang HQ, Wang CM, Cheng BH, et al. Apelin-13 inhibits apoptosis and excessive autophagy in cerebral ischemia/reperfusion injury. Neural Regen Res. 2021; 16(6):1044-51.
• Chen DD, Lee JW, Gu XH, Wei L, Yu SP. Intranasal Delivery of Apelin-13 Is Neuroprotective and Promotes Angiogenesis After Ischemic Stroke in Mice. Asn Neuro. 2015; 7(5):1759091415605114.
• Khoshsirat S, Abbaszadeh HA, Peyvandi AA, Heidari F, Peyvandi M, Simani L, et al. Apelin-13 prevents apoptosis in the cochlear tissue of noiseexposed rat via Sirt-1 regulation. J Chem Neuroanat. 2021;114:101956.
• Foussal C, Lairez O, Calise D, Pathak A, GuilbeauFrugier C, Valet P, et al. Activation of catalase by apelin prevents oxidative stress-linked cardiac hypertrophy. Febs Lett. 2010; 584(11):2363-70.