Ecological Life Sciences 1308-7258 E-Journal of New World Sciences Academy Molecular Identification of Parasites Isolated from Mediterranean Mussel (Mytilus Galloprovincialis Lamarck 1819) Specimens https://orcid.org/0000-0002-1143-4862 Yılmaz Harun ANKARA UNIVERSITY https://orcid.org/0000-0001-7474-2208 Genç Ercüment Ankara University https://orcid.org/0000-0003-3324-4493 Ünal Esra Mine ANKARA UNIVERSITY https://orcid.org/0000-0001-6411-8460 Yıldırım Şükrü EGE UNIVERSITY https://orcid.org/0000-0002-7279-313X Keskin Emre ANKARA UNIVERSITY 04 23 2020 15 2 61 71 12 06 2019 04 23 2020 Copyright © 2009, Ecological Life Sciences 2009 Ecological Life Sciences

In this study, Mediterranean mussels (Mytilus galloprovincialis Lamarck, 1819) were collected from the most important mussel distribution/fishing areas in Turkish coastal waters (Çanakkale, Balıkesir, İzmir). The presence of parasite was investigated based on molecular methods. Molecular identification of parasite species was established by designing parasite-specific primers for PCR amplification and resulting nucleotide sequences were analysed. As a result of this study, four parasite species were identified as Mytilicola intestinalis Steuer 1902, Mytilicola orientalis Mori 1935, Urastoma cyprinae Graff 1882, Parvatrema duboisi Dollfus 1923 respectively. All identified species were found in İzmir (Agean Sea) specimens while Urastoma cyprinae and Parvatrema duboisi were found only in Çanakkale (Dardanelle) and Balikesir (Marmara Sea) specimens. Nucleotide composition, pairwise genetic distance and phylogenetic trees of the detected species were given. This research takes place to be the first study on identifying mussel parasites by using molecular techniques. Also, Mytilicola orientalis is the first record from Turkish coastal waters.

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