The influence of ozonated saline on some physical and chemical parameters of blood serum: in vitro study

Year 2024, Volume: 41 Issue: 4, 685 - 689, 31.12.2024

Andrew Martusevich , Alexandra Surovegina Lida Kovaleva Alexey Davyduk Sergey Peretyagin Elvira Pocheshkhova

https://izlik.org/JA47GN92ZB

Abstract

This study aims to study the features of the initiated crystallogenesis of ozonated blood of healthy people, depending on the value of the saturating concentration of ozone. We studied the nature of the initiated crystallization of the "blood – ozonated saline solution" system (n=15) with an ozone concentration of 0; 1000 mcg/l; 3000 mcg/l; 6000 mcg/l; 10000 mcg/l; 20,000 mcg/l and 40,000 mcg/l. For the analysis, blood serum was mixed with aliquot amounts of 0.9% ozonated or controlled sodium chloride solution (component ratio 1: 1). The crystal formation result was evaluated using a set of semi-quantitative indicators. It has been established that human blood reacts most optimally to low doses of ozone (1000-6000 micrograms/l). The range of 6000-10000 micrograms/l is "borderline", and higher doses of ozone have an adverse effect on the biological fluid being studied.

Keywords

ozone , crystallogenic properties , blood , biocrystallomics

References

• Martusevich AK, Kosyuga SYu, Kovaleva LK, Fedotova AS, Tuzhilkin AN. Biocrystallomics as the basis of innovative biomedical technologies. New Armenian medical journal. 2023;17(2): 95-104.
• Martusevich AK, Kamakin NF. Crystallography of biological fluid as a method for evaluating its physico-chemical properties. Bulletin of Experimental Biology and Medicine. 2007;143(3):358-360.
• Deryabina NI, Zaleskij MG. The content of protein components in a drop of blood serum when it dries. Bulletin of New Medical Technologies. 2005;12(1):85.
• Yakhno T, Sanin A, Ilyazov R et al. Drying Drop Technology as a Possible Tool for Detection Leukemia and Tuberculosis in Cattle. Journal of Biomedical Science and Engineering. 2015;8:1-23.
• Plebani M, Banfi G, Bernardini S, et al. Serum or plasma? An old question looking for new answers. Clin Chem Lab Med. 2020;58(2):178-187.
• Dushkov VA, Kutnaya ZhB, Bajbulatova LB et al. Evaluation of the effectiveness of ozone therapy in patients with chronic cerebrovascular insufficiency based on the results of morphological analysis of blood serum. Nizhny Novgorod Medical Journal. 2005:79-81.
• Liu L, Zeng L, Gao L, Zeng J, Lu J. Ozone therapy for skin diseases: Cellular and molecular mechanisms. Int Wound J. 2023;20(6):2376-2385.
• Masan J, Sramka M, Rabarova D. The possibilities of using the effects of ozone therapy in neurology. Neuro Endocrinol Lett. 2021;42(1):13-21.
• Martusevich AK, Peretyagin SP, Ruchin MV, Struchkov AA. Ozone therapy in patients with burn disease. J. Biomedical Science and Engineering. 2018;11(2):27-35.
• Martusevich AK, Karuzin KA, Dilenyan LR, Peretyagin SP. Microcirculatory effects of systemic metabolic correction with reactive oxygen species: An experimental study. Biomedical Research and Therapy. 2020;7(10):4026-4031.
• Peretyagin SP, Dmitriev GI, Aminev VA et al. New medical technologies at the stages of rehabilitation of the burned. Medical almanac. 2010;2:221-224.
• Peretyagin SP, Struchkov AA, Martusevich AK et al. The use of ozone as a means of detoxification in the early period of burn disease. Emergency medical care. 2011;12(3):39-43.
• Wen Q, Liu D, Wang X, et al. A systematic review of ozone therapy for treating chronically refractory wounds and ulcers. Int Wound J. 2022;19(4):853-870.
• Zeng J, Lu J. Mechanisms of action involved in ozone-therapy in skin diseases. Int Immunopharmacol. 2018;56:235-241.
• Kontorshchikova KN et al. Biological mechanisms of the effectiveness of ozone therapy. Kazan Medical journal. 2007;88(S4):3.
• Peretyagin SP, Martusevich AK, Solovyeva AG et al. Enzymological evaluation of hepatotropic effect of ozone in a subchronic experiment. Bull Exp Biol Med. 2013;154(6):789-791.
• Rosul MV, Patskan BM. Ozone therapy effectiveness in patients with ulcerous lesions due to diabetes mellitus. Wiad Lek. 2016;69(1):7-9.
• Moreno-Fernández A, Macías-García L, Valverde-Moreno R, et al. Autohemotherapy with ozone as a possible effective treatment for fibromyalgia. Acta Reumatol Port. 2019