Naringin protects against colistin-induced sciatic nerve damage by reducing oxidative stress, apoptosis and inflammation damage

Abstract

Colistin is used in treatment against gram-negative bacteria. It causes toxic effects due to oxidative stress in a dose-dependent manner. Naringin is a flavonoid with strong antioxidant properties found naturally in many plants, especially citrus fruits. This study aimed to determine the protective effects of NRG in CLS-induced sciatic nerve injury. Thirty-five male Spraque rats were randomly divided into five groups: control, NRG, CLS, CLS+NRG50, and CLS+NRG100. CLS was administered orally at a 15 mg/kg dose for seven days. NRG was administered orally at 50 or 100 mg/kg doses for seven days. Sciatic nerve tissues were collected and analyzed for oxidative stress markers, inflammation, and apoptosis damage levels by biochemical methods. CLS caused toxic damage in sciatic nerve tissue by increasing lipid peroxidation (MDA) and decreasing antioxidant capacity (SOD, CAT, GPx, and GSH); increasing NF-κB, TNF-α, nNOS release; increasing apoptotic factor (Caspase-3); decreasing HO-1 and nCAM; and inducing 8-OHdG, MAPK and GFAP (p<0.05). NRG, on the other hand, reversed all these changes in sciatic nerve tissue and reduced CLS-induced oxidative stress damage, inflammatory damage and apoptotic damage (p<0.05). These effects were more effective at the 100 mg/kg dose of NRG than at the 50 mg/kg dose (p<0.05). In sciatic nerve tissue, CLS induced sciatic nerve toxicity with increased oxidative stress, inflammation, and apoptotic damage. NRG showed a protective effect against CLS-induced sciatic nerve toxicity.

Keywords

• Apoptosis
• Colistin
• Naringin
• Oxidative stress
• Sciatic nerve

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