Immunohistological examination of thyroid hormone receptor expressions in placenta in maternal hypothyroidism

Year 2025, Volume: 42 Issue: 2, 143 - 145, 30.06.2025

Gökçen Bilici , Serap Cilaker Mıcılı

Abstract

Thyroid hormones are necessary for the development of a healthy fetus and placenta. In our study, we evaluated the thyroid hormone receptor expressions in the placentas of pregnant women with hypothyroidism. We evaluated term placentas in two groups: control (n=5) and hypothyroidism (n=5). THRα and THRß were expressed in the placenta, especially in syncytiotrophoblasts, and also in placental macrophages. THRα expression was decreased in the hypothyroid group compared to the control group, but it was statistically non-significant (p=0,850) . THRß expression was decreased in the hypothyroid group compared to the control group at a statistically significant level (p=0,004). The results showed that thyroid hormone receptors are associated with maternal hypothyroidism.

Keywords

maternal hypothyroidism , placenta , thyroid hormone receptors

Project Number

22647

References

• Clines G, Demers L. General Endocrinology. In: Kaplan L, Pesce A, editors. Clinical Chemistry: Theory, Analysis, Correlation. 5th ed. St. Louis: Mosby Inc.; 2010.
• Kleerekoper M. Hormones. In: Burtis C, Ashwood E, Bruns D, editors. Tietz Fundamentals of Clinical Chemistry. 6th ed. Philadelphia: W.B. Saunders Company; 2008.
• Chiasera JM. Back to the basics: thyroid gland structure, function and pathology. Clin Lab Sci. 2013;26(2):112–7.
• Lasa M, Contreras-Jurado C. Thyroid hormones act as modulators of inflammation through their nuclear receptors. Front Endocrinol (Lausanne). 2022 Aug 8;13:937099.
• Ohara N, Tsujino T, Maruo T. The Role of Thyroid Hormone in Trophoblast Function, Early Pregnancy Maintenance, and Fetal Neurodevelopment. Journal of Obstetrics and Gynaecology Canada. 2004;26(11):982–90.
• Knabl J, de Maiziere L, Hüttenbrenner R, Hutter S, Jückstock J, Mahner S, et al. Cell Type- and Sex-Specific Dysregulation of Thyroid Hormone Receptors in Placentas in Gestational Diabetes Mellitus. International Journal of Molecular Sciences. 2020;21(11):4056.
• Kilby MD, Verhaeg J, Gittoes N, Somerset DA, Clark PMS, Franklyn JA. Circulating thyroid hormone concentrations and placental thyroid hormone receptor expression in normal human pregnancy and pregnancy complicated by intrauterine growth restriction (IUGR). J Clin Endocrinol Metab. 1998;83(8):2964–71.
• Korevaar TIM, Medici M, Visser TJ, Peeters RP. Thyroid disease in pregnancy: new insights in diagnosis and clinical management. Nature Reviews Endocrinology. 2017;13(10):610–22.
• Blatt AJ, Nakamoto JM, Kaufman HW. National Status of Testing for Hypothyroidism during Pregnancy and Postpartum. J Clin Endocrinol Metab. 2012;97(3):777–84.
• Lazarus J, Brown RS, Daumerie C, Hubalewska-Dydejczyk A, Negro R, Vaidya B. 2014 European Thyroid Association Guidelines for the Management of Subclinical Hypothyroidism in Pregnancy and in Children. Eur Thyroid J.2014;3(2):76.
• Abalovich M, Amino N, Barbour LA, Cobin RH, De Groot LJ, Glinoer D, et al. Management of Thyroid Dysfunction during Pregnancy and Postpartum: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2007;92(8_supplement):1–7.
• Demir N. Gebelikte tiroid hastaliklarinin tani ve yönetim kilavuzu. 2017.
• Toloza FJK, Abedzadeh-Anaraki S, Maraka S. Subclinical hypothyroidism in pregnancy. Curr Opin Endocrinol Diabetes Obes. 2019;26(5):225–31.
• van den Boogaard E, Vissenberg R, Land JA, van Wely M, van der Post JAM, Goddijn M, et al. Significance of (sub)clinical thyroid dysfunction and thyroid autoimmunity before conception and in early pregnancy: a systematic review. Hum Reprod Update. 2011;17(5):605–19
• Novakovic TR, Dolicanin ZC, Djordjevic NZ. Effects of maternal subclinical hypothyroidism on amniotic fluid cells oxidative status. Reproductive Toxicology. 2018;78:97–101.
• Zhao L, Jiang G, Tian X, Zhang X, Zhu T, Chen B, et al. Initiation timing effect of levothyroxine treatment on subclinical hypothyroidism in pregnancy. Gynecological Endocrinology. 2018;34(10):845–8.
• Castillo Lara M, Vilar Sánchez Á, Cañavate Solano C, Soto Pazos E, Iglesias Álvarez M, González Macías C, et al. “Hypothyroidism screening during first trimester of pregnancy.” BMC Pregnancy Childbirth. 2017;17(1):1–7.
• Nishioka E, Hirayama S, Ueno T, Matsukawa T, Vigeh M, Yokoyama K, et al. Relationship between maternal thyroid-stimulating hormone (TSH) elevation during pregnancy and low birth weight: A longitudinal study of apparently healthy urban Japanese women at very low risk. Early Hum Dev. 2015;91(3):181–5.
• Barber KJ, Franklyn JA, McCabe CJ, Khanim FL, Bulmer JN, Whitley GSJ, et al. The in Vitro Effects of Triiodothyronine on Epidermal Growth Factor-Induced Trophoblast Function. J Clin Endocrinol Metab. 2005;90(3):1655–61.
• Ziegelmüller B, Vattai A, Kost B, Kuhn C, Hofmann S, Bayer B, et al. Expression of Thyroid Hormone Receptors in Villous Trophoblasts and Decidual Tissue at Protein and mRNA Levels Is Downregulated in Spontaneous and Recurrent Miscarriages. Journal of Histochemistry and Cytochemistry. 2015;63(7):511–23.
• Perrotta C, Buldorini M, Assi E, Cazzato D, De Palma C, Clementi E, et al. The thyroid hormone triiodothyronine controls macrophage maturation and functions: Protective role during inflammation. American Journal of Pathology. 2014;184(1):230–47.
• Billon C, Canaple L, Fleury S, Deloire A, Beylot M, Dombrowicz D, et al. TRα Protects Against Atherosclerosis in Male Mice: Identification of a Novel Anti-Inflammatory Property for TRα in Mice. Endocrinology. 2014;155(7):2735–45.
• Furuya F, Ishii T, Tamura S, Takahashi K, Kobayashi H, Ichijo M, et al. The ligand-bound thyroid hormone receptor in macrophages ameliorates kidney injury via inhibition of nuclear factor-κB activities. Sci Rep. 2017;7:1–12.