Curcumin protects against ketoconazole-induced hepatotoxicity in Wistar rats

Bamiwuye Similoluwa; Nwakaego Ebong; Bamiwuye Similoluwa

Curcumin protects against ketoconazole-induced hepatotoxicity in Wistar rats

Abstract

Ketoconazole (KZ) is a broad-spectrum antifungal drug that can cause hepatotoxicity. Curcumin (CM), an isolate of Curcuma longa L. is used in folk medicine to treat various ailments. This study assessed whether CM can protect against a rat model of KZ-induced hepatotoxicity. Thirty healthy adult Wistar rats (220-240g) were randomly grouped (n=5/group) and supplemented orally with CM (50, 100 and 200 mg/kg/day) prior to the oral administration of KZ (200 mg/kg/day) for 14 days. On day 15, the rats were weighed and anesthetized. Blood samples were collected, and sera were extracted for biochemical analyses. Liver samples were excised, weighed and processed for oxidative stress markers and histology. KZ significantly (p < 0.01) decreased body weight and significantly (p< 0.001) decreased liver glutathione, superoxide dismutase, catalase, and glutathione peroxidase levels when compared to control. KZ significantly (p < 0.01) increased liver weight and significantly (p < 0.001) increased serum gamma-glutamyl transferase, alkaline phosphatase, amino transferases, lactate dehydrogenase, total bilirubin and liver malondialdehyde levels when compared to control. KZ caused hepatocyte necrosis. However, CM supplementation significantly restored body and liver weights at 50 mg/kg (p < 0.05), 100 mg/kg (p < 0.01) and 200 mg/kg (p < 0.01) when compared to KZ. CM supplementation restored serum biochemical and liver oxidative stress markers at 50 mg/kg (p < 0.05), 100 mg/kg (p < 0.01) and 200 mg/kg (p < 0.001) when compared to KZ. CM restored liver histology. CM was effective against KZ-induced hepatotoxicity in a dose-related fashion.

Keywords

Ethical Statement

The European Communities Council Directive of 24 November 1986 (86/609/EEC) was carefully and vividly followed during the course of this study as stated in the methodology section.

Thanks

Thanks for the privilege give to us to submit our research work.

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Details

Primary Language

English

Subjects

Gastroenterology and Hepatology

Journal Section

Research Article

Authors

Bamiwuye Similoluwa ^*
0000-0003-4349-8227
Nigeria

Nwakaego Ebong
0000-0002-8629-4390
Nigeria

Bamiwuye Similoluwa
0009-0005-5717-1649
Nigeria

Publication Date

June 30, 2025

Submission Date

August 28, 2024

Acceptance Date

April 16, 2025

Published in Issue

Year 2025 Volume: 42 Number: 2

IZ

https://izlik.org/JA95UH85KC

Cite

RIS / Bibtex

APA

Similoluwa, B., Ebong, N., & Similoluwa, B. (2025). Curcumin protects against ketoconazole-induced hepatotoxicity in Wistar rats. Deneysel Ve Klinik Tıp Dergisi, 42(2), 133-138. https://izlik.org/JA95UH85KC

AMA

1.Similoluwa B, Ebong N, Similoluwa B. Curcumin protects against ketoconazole-induced hepatotoxicity in Wistar rats. J. Exp. Clin. Med. 2025;42(2):133-138. https://izlik.org/JA95UH85KC

Chicago

Similoluwa, Bamiwuye, Nwakaego Ebong, and Bamiwuye Similoluwa. 2025. “Curcumin Protects Against Ketoconazole-Induced Hepatotoxicity in Wistar Rats”. Deneysel Ve Klinik Tıp Dergisi 42 (2): 133-38. https://izlik.org/JA95UH85KC.

EndNote

Similoluwa B, Ebong N, Similoluwa B (June 1, 2025) Curcumin protects against ketoconazole-induced hepatotoxicity in Wistar rats. Deneysel ve Klinik Tıp Dergisi 42 2 133–138.

IEEE

[1]B. Similoluwa, N. Ebong, and B. Similoluwa, “Curcumin protects against ketoconazole-induced hepatotoxicity in Wistar rats”, J. Exp. Clin. Med., vol. 42, no. 2, pp. 133–138, June 2025, [Online]. Available: https://izlik.org/JA95UH85KC

ISNAD

Similoluwa, Bamiwuye - Ebong, Nwakaego - Similoluwa, Bamiwuye. “Curcumin Protects Against Ketoconazole-Induced Hepatotoxicity in Wistar Rats”. Deneysel ve Klinik Tıp Dergisi 42/2 (June 1, 2025): 133-138. https://izlik.org/JA95UH85KC.

JAMA

1.Similoluwa B, Ebong N, Similoluwa B. Curcumin protects against ketoconazole-induced hepatotoxicity in Wistar rats. J. Exp. Clin. Med. 2025;42:133–138.

MLA

Similoluwa, Bamiwuye, et al. “Curcumin Protects Against Ketoconazole-Induced Hepatotoxicity in Wistar Rats”. Deneysel Ve Klinik Tıp Dergisi, vol. 42, no. 2, June 2025, pp. 133-8, https://izlik.org/JA95UH85KC.

Vancouver

1.Bamiwuye Similoluwa, Nwakaego Ebong, Bamiwuye Similoluwa. Curcumin protects against ketoconazole-induced hepatotoxicity in Wistar rats. J. Exp. Clin. Med. [Internet]. 2025 Jun. 1;42(2):133-8. Available from: https://izlik.org/JA95UH85KC