In vitro evaluation of colistin and ceftazidime avibactam activity against multi-drug resistant klebsiella pneumoniae isolates

Tuğba Avan Mutlu

In vitro evaluation of colistin and ceftazidime avibactam activity against multi-drug resistant klebsiella pneumoniae isolates

Abstract

This study aimed to evaluate the in vitro effectiveness of colistin and ceftazidime-avibactam (CZA) antibiotics against multidrug-resistant (MDR) Klebsiella pneumoniae isolates. The study included 54 clinical isolates sent to the Medical Microbiology Laboratory of Kirsehir Education and Research Hospital between 2022 and 2023, which were resistant to multiple drugs in routine antibiotic susceptibility tests. Colistin susceptibility was evaluated using the microdilution method, while CZA susceptibility was assessed using the disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria. It was determined that 31.4% of the isolates were resistant to colistin, and 22.2% were resistant to CZA. No isolates were resistant to both antibiotics. Statistical analyses did not reveal a significant relationship between antibiotic susceptibility and gender, age, underlying disease, and sample type (p>0.05). However, colistin resistance has been found to be higher in intensive care units, whereas resistance to CZA has significantly increased in internal medicine units (p =0.025). The study's findings indicate that colistin and CZA may be important in treating multidrug-resistant K. pneumoniae infections. CZA provides an effective alternative for colistin-resistant isolates, while colistin is an effective alternative for CZA-resistant isolates. However, to enhance the efficacy of these agents, larger-scale studies and ongoing monitoring of resistance mechanisms are necessary.

Keywords

Supporting Institution

No financial support was received for the project.

Ethical Statement

It was obtained from Kirsehir Ahi Evran University Faculty of Medicine, Non-Interventional Clinical Research Ethics Committee with the decision number 2024-4/15 dated 06/02/2024.

References

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Details

Primary Language

English

Subjects

Clinical Microbiology, Medical Bacteriology

Journal Section

Research Article

Authors

Tuğba Avan Mutlu ^*
0000-0001-7242-9971
Türkiye

Publication Date

June 30, 2025

Submission Date

March 31, 2025

Acceptance Date

June 4, 2025

Published in Issue

Year 2025 Volume: 42 Number: 2

IZ

https://izlik.org/JA75FM89AU

Cite

RIS / Bibtex

APA

Avan Mutlu, T. (2025). In vitro evaluation of colistin and ceftazidime avibactam activity against multi-drug resistant klebsiella pneumoniae isolates. Deneysel Ve Klinik Tıp Dergisi, 42(2), 159-164. https://izlik.org/JA75FM89AU

AMA

1.Avan Mutlu T. In vitro evaluation of colistin and ceftazidime avibactam activity against multi-drug resistant klebsiella pneumoniae isolates. J. Exp. Clin. Med. 2025;42(2):159-164. https://izlik.org/JA75FM89AU

Chicago

Avan Mutlu, Tuğba. 2025. “In Vitro Evaluation of Colistin and Ceftazidime Avibactam Activity Against Multi-Drug Resistant Klebsiella Pneumoniae Isolates”. Deneysel Ve Klinik Tıp Dergisi 42 (2): 159-64. https://izlik.org/JA75FM89AU.

EndNote

Avan Mutlu T (June 1, 2025) In vitro evaluation of colistin and ceftazidime avibactam activity against multi-drug resistant klebsiella pneumoniae isolates. Deneysel ve Klinik Tıp Dergisi 42 2 159–164.

IEEE

[1]T. Avan Mutlu, “In vitro evaluation of colistin and ceftazidime avibactam activity against multi-drug resistant klebsiella pneumoniae isolates”, J. Exp. Clin. Med., vol. 42, no. 2, pp. 159–164, June 2025, [Online]. Available: https://izlik.org/JA75FM89AU

ISNAD

Avan Mutlu, Tuğba. “In Vitro Evaluation of Colistin and Ceftazidime Avibactam Activity Against Multi-Drug Resistant Klebsiella Pneumoniae Isolates”. Deneysel ve Klinik Tıp Dergisi 42/2 (June 1, 2025): 159-164. https://izlik.org/JA75FM89AU.

JAMA

1.Avan Mutlu T. In vitro evaluation of colistin and ceftazidime avibactam activity against multi-drug resistant klebsiella pneumoniae isolates. J. Exp. Clin. Med. 2025;42:159–164.

MLA

Avan Mutlu, Tuğba. “In Vitro Evaluation of Colistin and Ceftazidime Avibactam Activity Against Multi-Drug Resistant Klebsiella Pneumoniae Isolates”. Deneysel Ve Klinik Tıp Dergisi, vol. 42, no. 2, June 2025, pp. 159-64, https://izlik.org/JA75FM89AU.

Vancouver

1.Tuğba Avan Mutlu. In vitro evaluation of colistin and ceftazidime avibactam activity against multi-drug resistant klebsiella pneumoniae isolates. J. Exp. Clin. Med. [Internet]. 2025 Jun. 1;42(2):159-64. Available from: https://izlik.org/JA75FM89AU