Anticancer effect of total yellow hawthorn extract on chronic myeloid leukemia cells and acute myeloid leukemia cells

Ayşe Nur Arslan; İsmail Akçok

Anticancer effect of total yellow hawthorn extract on chronic myeloid leukemia cells and acute myeloid leukemia cells

Abstract

Cancer is a disease characterized by abnormal cell growth and invasion and metastasis of these cells to other tissues or organs of the body. Natural products have been used for centuries as drugs or in drug development, especially for the treatment of cancer. Besides, extracting natural products with several bioactive compounds has a promising effect on cancer treatment. In this study, we aimed to investigate the anticancer effect of the ethanolic extract of yellow hawthorn fruits on K562 (Chronic Myeloid Leukemia) and MOLM-13 (Acute Myeloid Leukemia) cell lines. The antiproliferative effect of the ethanolic extract of yellow hawthorn fruits was investigated in time- and dose-dependent manners. The Annexin-V/Propidium Iodide (PI) double staining was used to examine the apoptosis. Furthermore, cell cycle analysis is conducted by PI staining. The cell viability of K562 and MOLM-13 cell lines was significantly reduced by the ethanolic extract of yellow hawthorn fruits with IC50 values of 9144 µg/mL and 3515 µg/mL in 48-hour incubation time, respectively. Moreover, the results showed that the ethanolic extract of yellow hawthorn fruits caused an increased apoptosis by 12.7- and 8.87-fold changes in K562 and MOLM-13 cell lines compared to control groups, respectively. Ethanolic extract of yellow hawthorn fruit has reduced cell proliferation, induced apoptosis and arrested the cell cycle at G0/G1 phase by 71% in MOLM-13 and at G2/M phase by 80.3% and G0/G1 phase by 38.2 % in K562 cells. Further studies should be conducted to elucidate the mechanism of the effect of yellow hawthorn fruit on these cancer cells.

Keywords

Supporting Institution

TUBİTAK

Project Number

1919B012301267

Thanks

This study is supported by The Scientific and Technological Research Council of Türkiye (TUBITAK) with project number 1919B012301267 (2209-A).

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Details

Primary Language

English

Subjects

Pharmaceutical Chemistry, Cancer Cell Biology, Chemotherapy

Journal Section

Research Article

Authors

Ayşe Nur Arslan
0009-0008-9652-9895
Türkiye

İsmail Akçok ^*
0000-0002-5444-3929
Türkiye

Publication Date

June 30, 2025

Submission Date

February 1, 2025

Acceptance Date

June 27, 2025

Published in Issue

Year 2025 Volume: 42 Number: 2

IZ

https://izlik.org/JA48BU48YS

Cite

RIS / Bibtex

APA

Arslan, A. N., & Akçok, İ. (2025). Anticancer effect of total yellow hawthorn extract on chronic myeloid leukemia cells and acute myeloid leukemia cells. Deneysel Ve Klinik Tıp Dergisi, 42(2), 119-127. https://izlik.org/JA48BU48YS

AMA

1.Arslan AN, Akçok İ. Anticancer effect of total yellow hawthorn extract on chronic myeloid leukemia cells and acute myeloid leukemia cells. J. Exp. Clin. Med. 2025;42(2):119-127. https://izlik.org/JA48BU48YS

Chicago

Arslan, Ayşe Nur, and İsmail Akçok. 2025. “Anticancer Effect of Total Yellow Hawthorn Extract on Chronic Myeloid Leukemia Cells and Acute Myeloid Leukemia Cells”. Deneysel Ve Klinik Tıp Dergisi 42 (2): 119-27. https://izlik.org/JA48BU48YS.

EndNote

Arslan AN, Akçok İ (June 1, 2025) Anticancer effect of total yellow hawthorn extract on chronic myeloid leukemia cells and acute myeloid leukemia cells. Deneysel ve Klinik Tıp Dergisi 42 2 119–127.

IEEE

[1]A. N. Arslan and İ. Akçok, “Anticancer effect of total yellow hawthorn extract on chronic myeloid leukemia cells and acute myeloid leukemia cells”, J. Exp. Clin. Med., vol. 42, no. 2, pp. 119–127, June 2025, [Online]. Available: https://izlik.org/JA48BU48YS

ISNAD

Arslan, Ayşe Nur - Akçok, İsmail. “Anticancer Effect of Total Yellow Hawthorn Extract on Chronic Myeloid Leukemia Cells and Acute Myeloid Leukemia Cells”. Deneysel ve Klinik Tıp Dergisi 42/2 (June 1, 2025): 119-127. https://izlik.org/JA48BU48YS.

JAMA

1.Arslan AN, Akçok İ. Anticancer effect of total yellow hawthorn extract on chronic myeloid leukemia cells and acute myeloid leukemia cells. J. Exp. Clin. Med. 2025;42:119–127.

MLA

Arslan, Ayşe Nur, and İsmail Akçok. “Anticancer Effect of Total Yellow Hawthorn Extract on Chronic Myeloid Leukemia Cells and Acute Myeloid Leukemia Cells”. Deneysel Ve Klinik Tıp Dergisi, vol. 42, no. 2, June 2025, pp. 119-27, https://izlik.org/JA48BU48YS.

Vancouver

1.Ayşe Nur Arslan, İsmail Akçok. Anticancer effect of total yellow hawthorn extract on chronic myeloid leukemia cells and acute myeloid leukemia cells. J. Exp. Clin. Med. [Internet]. 2025 Jun. 1;42(2):119-27. Available from: https://izlik.org/JA48BU48YS