Investigation of the diagnostic value of CMV DNA positivity in different clinical samples

Canberk Çınar; Sedanur Karaahmet; Yeliz Tanrıverdi Çaycı; Demet Gür Vural; Kemal Bilgin; Asuman Bırıncı

Investigation of the diagnostic value of CMV DNA positivity in different clinical samples

Abstract

Cytomegalovirus, a common herpesvirus, is often acquired during childhood or adolescence. After viral DNA integrates into the host, lifelong latency can occur as latent infection in various organs, including the intestines, without causing disease. However, in immunocompromised individuals, primary infection or reactivation of the latent virus can lead to serious illnesses. Histological and serological tests are used for the diagnosis of cytomegalovirus infection. In addition, detecting cytomegalovirus DNA in plasma or biopsy samples via Polymerase Chain Reaction is also important. In this study, a retrospective analysis was conducted on 200 patients whose samples were sent to the Molecular Microbiology Laboratory of Ondokuz Mayıs University Faculty of Medicine for Cytomegalovirüs Polymerase Chain Reaction testing between 2023 and 2025. Patients who had both plasma and tissue samples sent simultaneously were included in the study. Of the tissue samples analyzed, 92.5% were colonoscopy materials obtained from patients diagnosed with inflammatory bowel disease. The number of patients with both plasma and tissue positivity was 42, 30 patients were tissue positive but plasma negative, and 15 patients were plasma positive but tissue negative. In 113 patients, Cytomegalovirus DNA was not detected in both samples. Detection of cytomegalovirus DNA in plasma does not provide sufficient information for the diagnosis of cytomegalovirus infection, which is a tissue-tropic virus. Performing this test on tissue samples with clinical symptoms may provide more valuable diagnostic information.

Keywords

References

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Details

Primary Language

English

Subjects

Medical Virology

Journal Section

Research Article

Authors

Canberk Çınar ^*
0000-0002-8355-7749
Türkiye

Sedanur Karaahmet
0009-0002-4828-5220
Türkiye

Yeliz Tanrıverdi Çaycı
0000-0002-9251-1953
Türkiye

Demet Gür Vural
0000-0003-2974-6589
Türkiye

Kemal Bilgin
0000-0002-8892-2223
Türkiye

Asuman Bırıncı
0000-0002-8653-4710
Türkiye

Publication Date

September 30, 2025

Submission Date

March 26, 2025

Acceptance Date

August 1, 2025

Published in Issue

Year 2025 Volume: 42 Number: 3

IZ

https://izlik.org/JA27YF74GL

Cite

RIS / Bibtex

APA

Çınar, C., Karaahmet, S., Tanrıverdi Çaycı, Y., Gür Vural, D., Bilgin, K., & Bırıncı, A. (2025). Investigation of the diagnostic value of CMV DNA positivity in different clinical samples. Deneysel Ve Klinik Tıp Dergisi, 42(3), 274-278. https://izlik.org/JA27YF74GL

AMA

1.Çınar C, Karaahmet S, Tanrıverdi Çaycı Y, Gür Vural D, Bilgin K, Bırıncı A. Investigation of the diagnostic value of CMV DNA positivity in different clinical samples. J. Exp. Clin. Med. 2025;42(3):274-278. https://izlik.org/JA27YF74GL

Chicago

Çınar, Canberk, Sedanur Karaahmet, Yeliz Tanrıverdi Çaycı, Demet Gür Vural, Kemal Bilgin, and Asuman Bırıncı. 2025. “Investigation of the Diagnostic Value of CMV DNA Positivity in Different Clinical Samples”. Deneysel Ve Klinik Tıp Dergisi 42 (3): 274-78. https://izlik.org/JA27YF74GL.

EndNote

Çınar C, Karaahmet S, Tanrıverdi Çaycı Y, Gür Vural D, Bilgin K, Bırıncı A (September 1, 2025) Investigation of the diagnostic value of CMV DNA positivity in different clinical samples. Deneysel ve Klinik Tıp Dergisi 42 3 274–278.

IEEE

[1]C. Çınar, S. Karaahmet, Y. Tanrıverdi Çaycı, D. Gür Vural, K. Bilgin, and A. Bırıncı, “Investigation of the diagnostic value of CMV DNA positivity in different clinical samples”, J. Exp. Clin. Med., vol. 42, no. 3, pp. 274–278, Sept. 2025, [Online]. Available: https://izlik.org/JA27YF74GL

ISNAD

Çınar, Canberk - Karaahmet, Sedanur - Tanrıverdi Çaycı, Yeliz - Gür Vural, Demet - Bilgin, Kemal - Bırıncı, Asuman. “Investigation of the Diagnostic Value of CMV DNA Positivity in Different Clinical Samples”. Deneysel ve Klinik Tıp Dergisi 42/3 (September 1, 2025): 274-278. https://izlik.org/JA27YF74GL.

JAMA

1.Çınar C, Karaahmet S, Tanrıverdi Çaycı Y, Gür Vural D, Bilgin K, Bırıncı A. Investigation of the diagnostic value of CMV DNA positivity in different clinical samples. J. Exp. Clin. Med. 2025;42:274–278.

MLA

Çınar, Canberk, et al. “Investigation of the Diagnostic Value of CMV DNA Positivity in Different Clinical Samples”. Deneysel Ve Klinik Tıp Dergisi, vol. 42, no. 3, Sept. 2025, pp. 274-8, https://izlik.org/JA27YF74GL.

Vancouver

1.Canberk Çınar, Sedanur Karaahmet, Yeliz Tanrıverdi Çaycı, Demet Gür Vural, Kemal Bilgin, Asuman Bırıncı. Investigation of the diagnostic value of CMV DNA positivity in different clinical samples. J. Exp. Clin. Med. [Internet]. 2025 Sep. 1;42(3):274-8. Available from: https://izlik.org/JA27YF74GL