Comparison of the protective effects of betamethasone, dexamethasone and methylprednisolone in ischemia/reperfusion injury of rat ovary

Abstract

Abstract Objectives Our aim is to evaluate the protective effect of dexamethasone, methylprednisolone and betamethasone treatment against ischemia-reperfusion damage created experimentally in rat ovaries. Methods For this study, 30 female Wistar albino rats were used, and the rats were separated randomly into five groups consisting of six rats each: normal ovary, torsion-detorsion, torsion-detorsion + betamethasone 3 mg/kg, torsion-detorsion + dexamethasone 4 mg/kg and torsion-detorsion + metilprednisolone 10 mg/kg. Except for the normal group, an ovarian torsion procedure was implemented in all other groups for 3 hours. Then, a detorsion procedure was implemented to the groups for 3 hours. Medications were given intraperitoneally, 30 minutes before the detorsion procedure. Ovaries of all rats were removed and anti-mullerian hormone (AMH) levels were examined. Results The methylprednisolone treatment seems to be protective for the damage in terms of vascular congestion (p= 0.238), inflammation (p= 0.575), edema (p= 0.118) and cellular degeneration (p= 0.523) by preventing the meaningful increase. The dexamethasone and betamethasone treatment seems to be protective for tissue damage in inflammation (p= 0.575, 0.299), cellular degeneration (p= 0.575, 0.368) and edema (p= 0.212, 0.162). For all steroid groups, preantral+antral follicle decrease and atretic follicle increase was prevented. AMH decline was prevented and levels were similar to normal group (methylprednisolone, betamethasone and dexamethasone p values, respectively;0.872, 0.064, 0.335) Conclusions In ischemia / reperfusion injury due to ovarian torsion, steroid use reduces damage and protects ovarian reserves. There was no significant difference between dexamethasone, betamethasone and methylprednisolone in terms of success.

Keywords

• Ovarian torsion,Ischemia,Methylprednisolone,Dexamethasone,Betamethasone

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