Protective effect of melatonin on the rat lung following exposure to a 900-MHz electromagnetic field: a stereological and histopathological study

Abstract

Long-term use of cell phone has increased concerns regarding histopathological change in vital organs. Our present study was aimed to investigate the effect of EMF on the rat lungs, as well as the possible efficacy of melatonin (MEL) on lung tissues. Fifteen female wistar albino rats were randomly selected and assigned into five groups as follow: control (CONT), SHAM, EMF, melatonin (MEL) and EMF+MEL group. Subsequently, female rats were then mated, and pregnant rats underwent the experimental application for 21 days. After parturition, 6 pups (2 pups from each mother) were randomly chosen and maintained for 4 weeks. At the end of the 4th week, the lung tissues of all groups were used for the stereological analysis and histopathological examination. We found that the mean volumes of the alveoli, bronchioles and blood vessels were significantly lower in the EMF group compared to the control group (p < 0.05). In the EMF+MEL group, the mean volumes of the alveoli, bronchioles and blood vessels were significantly increased compared to the EMF group. Our histopathological results showed marked change in the lung tissues. We speculated that exposure to EMF caused the damage to the rat lung tissues, and that MEL administration alleviated 900 MHz EMF-induced complications.

Keywords

• Electromagnetic field,melatonin,lung,stereology,rat

References

1. Altunkaynak, B.Z., Altunkaynak, M.E, 2007. Relationship of body weight and volume of liver. A morphometrical and stereological study. Saudi. Med. J. 28, 891-895.
2. Altunkaynak, B.Z., Önger, M.E., Altunkaynak, M.E., Ayrancı, E., Canan, S., 2012. A brief introduction to stereology and sampling strategies: Basic concepts of stereology. Neuroquantology. 10, 31-43.
3. Ames, B.N., Shingenega, M.K., Hagen, T.M., 1993. Oxidants, antioxidants, and the degenerative disease of aging. Proc. Natl. Acad. Sci. USA. 90, 7915-7922.
4. Baltaci, A.K., Mogulkoc, R., Salbacak, A., Celik, I., Sivrikaya, A., 2012. The role of zinc supplementation in the inhibition of tissue damage caused by exposure to electromagnetic field in rat lung and liver tissues. Bratisl. Lek. Listy.113, 400-403.
5. Brennan, C.P., Hendricks, G.L., El-Sheikh, T.M., Mashaly, M.M., 2002. Melatonin and the enhancement of immune responses in immature male chickens. Poult. Sci. 81, 371–375. Brzezinski, A., 1997. MEL in humans. N. Engl. J. Med. 336, 186–195.
6. Davies, K.J., 1999. The broad spectrum of responses to oxidants in proliferating cells: a new paradigm for oxidative stress. IUBMB. Life. 48, 41-47.
7. Dursun, H., Albayrak F,, Uyanik, A., Keleş, N.O., Beyzagül, P., Bayram, E., Halici, Z., Altunkaynak, Z.B., Süleyman, H., Okçu, N., Ünal, B., 2010. Effects of hypertension and ovariectomy on rat hepatocytes. Are amlodipine and lacidipine protective? (A stereological and histological study). Turk. J. Gastroenterol. 21, 387-395.
8. Farías, J.G., Zepeda, A.B., Calaf, G.M., 2012. Melatonin protects the heart, lungs and kidneys from oxidative stress under intermittent hypobaric hypoxia in rats. Biological. research. 45, 81-85. Fuenta, A., Rojas, J., Flores, L., Gonzaler, J., 2008. Influence of 60Hz magnetic fields on growth and differentiation. Exp. Parasitol. 119, 200-206.

9. Gundersen, H.J., Jensen, E.B., 1987. The efficiency of systematic sampling in stereology and its prediction. J. Microsc. 147, 229-263. Inoue, M., Miller, M.W., Carstensen, E.L., Brayman, A.A., 1985. The relationship between sensivity to 60 Hz electric fields and induced transmembrane potentials in plant root cell. Radiat. Environ. Biophys. 24, 303-314.
10. Jahnke, G., Marr, M., Myers, C., Wilson, R., Travlos, G., Price, C., 1999. Maternal and developmental toxicity evaluation of melatonin administered orally to pregnant Sprague Dawley rats. Toxicologic. Sci. 50, 271–279.
11. Kaya, H., Delibas, N., Serteser, M., Ulukaya, E., Ozkaya, O., 1999. The effect of melatonin on lipid peroxidation during radiotherapy in female rats. Strahlenther. Onkol. 175, 285–288.
12. Koyu, A., Cesur, G., Ozguner, F., Akdogan, M., Mollaoglu, H., Ozen, S., 2005. Effects of 900 MHz electromagnetic field on TSH and thyroid hormones in rats. Toxicol. Lett. 157, 257–262.
13. Marron, M.T., Goodmann, E.M., Greenebaum, B., Tipnis, P., 1986. Effects of sinusoidal 60 Hz electric and magnetic fields on ATP and oxygen levels in the slime Mold, Physarum polycephalum. Bioelectromagnetics. 7, 307-314.
14. McCann, J., Dietrich, F., Rafferty, C., 1998. The genotoxic potential of electric and magnetic fields: an update. Mutat. Res. 411, 45-86.
15. Okatani, Y., Wakatsuki, A., Shinohara, K., Kaneda, C., Fukaya, T., 2001. Melatonin stimulates glutathione peroxidase activity in human chorion. J. Pineal. Res. 30, 199–205.
16. Reiter, R.J., 1991. Pineal melatonin: Cell biology of its synthesis and of its physiological interactions. Endocr. Rev. 12, 151-180.
17. Reiter, R.J., 1995. The pineal gland and melatonin in relation to aging: A summary of the theories and of the data. Exp. Gerontol. 30, 199–212.
18. Repacholi, M.H., 2001. Health risks from the use of mobile phones. Toxicol. Lett. 120, 323–331.
19. Rodriguez, C., Mayo, J.C., Sainz, R.M., Antolin, I., Herrera, F., Martin, V., Reiter, R.J., 2004. Regulation of Antioxidant Enzymes: A Significant Role for Melatonin. J. Pineal. Res. 36, 1-9. Selmaoui, B., Lambrozo, J., Touitou, Y., 1997. Endocrine function in young men exposed for one night to a 50Hz magnetic field. A Circadian Study of Pituitary, Thyroid and Adrenocortical Hormones. Life. Sci. 61, 473-484.
20. Selmaoui, B., Bogdan, A., Auzeby, A., Lambrozo, J., 1999. Acute exposure to 50 Hz magnetic field does not affect hematologic or immunologic functions in healthy young men: a circadian study. Bioelectromagnetics.1, 364-372.
21. Sirav, B., Seyhan, N., 2011. Effects of radiofrequency radiation exposure on the blood brain barrier permeability in male and female rats. Electromag. Biol. Med. 30, 253–260.
22. Takhtfooladi, H., Takhtfooladi, M., Moayer, F., Mobarakeh, S., 2015. Melatonin attenuates lung injury in a hind limb ischemia-reperfusion rat model. Rev. Port. Pneumol. (2006). 21, 30-35.
23. Torres, F., González-Candia, A., Montt, C., Ebensperger, G., Chubretovic, M., Serón-Ferré, M., Reyes, R.V., Llanos, A.J., Herrera, E.A., 2015. Melatonin reduces oxidative stress and improves vascular function in pulmonary hypertensive newborn sheep. J. Pineal. Res. 58, 362-373.
24. Tahamtan, R., Shabestani Monfared, A., Tahamtani, Y., Tavassoli, A., Akmali, M., Mosleh-Shirazi, M.A., Naghizadeh, M.M., Ghasemi, D., Keshavarz, M., Haddadi, G.H., 2015. Radioprotective effect of melatonin on radiation-induced lung injury and lipid peroxidation in rats. Cell. J. 17 ,111-120.
25. Tan, D.X., Manchester, L.C., Reiter, R.J., Plummer, B.F., Limson, J., Weintraub, S.T., Qi, W, 2000. Melatonin directly scavenges hydrogen peroxide: a potentially new metabolic pathway of melatonin biotransformation. Free. Radic. Biol. Med. 29, 1177-1185.
26. Tang, J., Zhang, Y., Yang, L., Chen, Q., Tan, L., Zuo, S., Feng, H., Chen, Z., Zhu, G., 2015. Exposure to 900 MHz electromagnetic fields activates the mkp-1/ERK pathway and causes blood-brain barrier damage and cognitive impairment in rats. Brain. Research. 1601, 92-101.
27. Tenuzzo, B., Vergallo, C., Dini, L., 2009. Effects of 6mT static magnetic field on the bc2-bax, p53 and hsp 70 expression in freshly isolated in vitro aged human lymphocytes. Tissue. Cell. 41, 169-179.
28. Ulubay, M., Yahyazadeh, A., Deniz, Ö.G., Kıvrak, E.G., Altunkaynak, B.Z., Erdem, G., Kaplan, S., 2014. Effects of prenatal 900 MHz electromagnetic field exposures on the histology of rat kidney. Int. J. Radiat. Biol. 1, 1-25. Wolf, F.I., Torsello, A., Tedesco, B., Fasanella, S., Boninsegna, A., D'Ascenzo, M., Grassi, C., Azzena, G.B., Cittadini, A., 2005. Hz extremely low frequency electromagnetic fields enhance cell proliferation and DNA damage: possible involvement of a redox mechanism. Biochim. Biophys. Acta. 1743, 120-129.
29. Yahyazedeh, A., Altunkaynak, B.Z., Akgül, N., Akgül, H.M., 2017. A histopathological and stereological study of liver damage in female rats caused by mercury vapor. Biotech. Histochem. 92, 338-346.
30. Yahyazadeh, A., Altunkaynak, B.Z., 2019a. Protective effects of luteolin on rat testis following exposure to 900 MHz electromagnetic field. Biotech. Histochem. 1-10.
31. Yahyazadeh, A., Altunkaynak, B.Z., 2019b. Investigation of the neuroprotective effects of thymoquinone on rat spinal cord exposed to 900 MHz electromagnetic field. J. Chem. Neuroanat. 100, 101657.
32. Yahyazadeh A, Altunkaynak B.Z., Kaplan, S., 2020. Biochemical, Immunohistochemical and Morphometrical Investigation of the Effect of Thymoquinone on the Rat Testis Following Exposure to a 900-MHz Electromagnetic Field. Acta. Histochem. 122, 151467.