Stereological Examination of the Effect on the Hippocampal Dentate Gyrus Granule Cells Number of Rats Subjected to Wireless Internet During Prenatal Period

Abstract

As a result of industrialisation and the many recent advances in technology, we are being intensely subjected to non-ionised radiation sources. As such, the effects of non-ionised radiation on human tissue are now being researched. In this study, the effects of Wi-Fi waves found in some third-generation mobile phones to facilitate internet connection—on the brain hippocampal dentate gyrus granule cell quantity of rats during the prenatal period was stereologically examined. During the study, mated albino rats were subjected to Wi-Fi throughout pregnancy. A month after giving birth, six rats from each group, for a total of 12 rats, was sacrificed under perfusion and anaesthesia. Their skulls were opened and brains were removed to undergo routine checks in which the granule cells were counted. Strategic sections were defined, and every 45th section couple (with a thickness of 5 µm) was taken and dyed with haematoxylin and eosin (H&E) and cresyl violet stain. The granule cells were counted using a combination of the stereological dissector method and Cavalier’s principle. The results were analysed using the Mann–Whitney U test. No statistically significant difference has been observed between the groups (p > 0.05). The findings were discussed in light of the relevant literature, and it was determined that throughout pregnancy, Wi-Fi modem device did result in any changes in the dentate gyrus granule cell count of the hippocampus of postnatal rats’ brains.

Keywords

• Wi-fi
• Cerebellar Granule Cells Numbers
• Rats Prenatal Period

References

1. Alchalabi ASH, Aklilu E, Aziz AR, Malek F, Ronald SH and Khan MA.,2016. Different periods of intrauterine exposure to electromagnetic field: Influence on female rats' fertility, prenatal and postnatal development. Asian Pacific Journal of Reproduction, 5, 1, 14–23.
2. Baan R, Grosse Y, Lauby-Secretan B, El Ghissassi F, Bouvard V, Benbrahim-Tallaa L, Guha N, Islami F, Galichet L and Straif K., 2011. Carcinogenicity of radiofrequency electromagnetic fields. Lancet Oncol, 12, 7, 624–6.
3. Balıkçı K, Serhatlioğlu I, Kutlu S, Poyraz M., 2016. Effect of electromagnetıc field exposure in vivo on catecholaminergic neurotransmitter concentrations in brain tissues in rats. Medical Sciences. 11(3): 1-8
4. Chung MK, Lee SJ, Kim YB, Park SC, Shin DH, Kim SH and Kim JC., 2005. Evaluation of spermatogenesis and fertility in F1 male rats after in utero and neonatal exposure to extremely low frequency electromagnetic fields. Asian J Androl, 7, 2, 189-94.
5. Çelik Ö, Kahya MC, Nazıroğlu M., 2016. Oxidative stress of brain and liver is increased by Wi-Fi (2.45GHz) exposure of rats during pregnancy and the development of newborns. J. Chemical. Neuroanatomy. 75(Pt B):134-9. doi: 10.1016/j.jchemneu.2015.10.005. Epub 2015 Oct 28.
6. Daniels WM, Pitout IL, Afullo TJ and Mabandla MV., 2009. The effect of electromagnetic radiation in the mobile phone range on the behaviour of the rat. Metab Brain Dis, 24, 4, 629-641.
7. Foster, K.R., Moulder, J.E., 2013. Wi-Fi and health: review of current status of research. Health Phys. 105, 561–565.
8. Gundersen HJ and Jensen EB., 1987. The efficiency of systematic sampling in stereology and its prediction. J. Microsc, 147, 229-63.

9. Gundersen HJG, Bendtsen TF, Korbo L, Marcussen N, Moller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sorensen FB, Vesterby A and West MJ., 1988. Some new simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS, 96, 5, 379-394.
10. Hardell L, Mild Kh, Carlberg M and Söderqvist F., 2006. Tumour risk associated with use of cellular telephones or cordless desktop telephones. World Journal of Surgical Oncology, 4, 74.
11. He WB, Zhang JL, Hu JF, Zhang Y, Machida T and Chen NH., 2008. Effects of glucocorticoids on age-related impairments of hippocampal structure and function in mice. Cell Mol Neurobiol, 28, 2, 277–291.
12. Hines M., 2006. Prenatal testosterone and gender-related behaviour. European journal of endocrinology. 155, 1, 115-121.
13. Howard CV and Reed MG., 1998. Unbiased Stereology Three-Dimensional Measurement in Microscopy. BIOS Scientific Publishers, Oxford, UK. Khurana V, Moulder J and Orton C., 2008. There is currently enough evidence and technology available to warrant taking immediate steps to reduce exposure of consumers to cell-phone-related electromagnetic radiation. Med. Phys, 35, 12, 5203-5206.
14. Khurana V, Teo C, Kundi M, Hardell L and Carlberg M., 2009. Cell phones and brain tumors: a review including the long-term epidemiologic data. Surg. Neurol, 72, 205–214.
15. Kiray M., 2010. The immunohistochemicaland structural effects of carnitine on oxidative stress in electromagnetic field exposed rat brains. Dokuz eylul university medical school departments of Histology&Embryology. Ph.D. Thesis, İzmir-Turkey.
16. Kundi M., 2009. The controversy about a possible relationship between mobile phone use and cancer. Environ Health Perspect, 117, 3, 316–24.
17. Lai H, Singh NP., 1997. Acute exposure to a 60 Hz magnetic field increases DNA strand breaks in rat brain cells. Bioelectromagnetics 18:156–165.
18. Moore KL, Persaud TVN and Torchia MG., 2013. The Developing Human Clinically Oriented Embryology. Elsevier Saunders, 9th Edition, Canada.
19. Odacı E, Baş O and Kaplan S., 2008. Effects of prenatal exposure to a 900 Mhz electromagnetic field on the dentate gyrus of rats: a stereological and histopathological study. Brain Research, 1238, 224-229.
20. Odacı E, Hancı H, İkinci A, Sönmez O.F, Aslan A, Şahin A, Kaya H, Çolakoğlu S, Baş O., 2016. Maternal exposure to a continuous 900-MHz electromagnetic field provokes neuronal loss and pathological changes in cerebellum of 32-day-old female rat offspring. Journal of Chemical Neuroanatomy. Volume 75, Part B, Pages 105-110.
21. Ohtani S, Ushiyama A, Maeda M, Ogasawara Y, Wang J, Kunugita N and Ishii K., 2015. The effects of radio-frequency electromagnetic fields on T cell function during development. J Radiat Res, 56, 3, 467-474.
22. Pall M L., 2018. Wi-Fi is an important threat to human health. Environmental Research, 164 405–416.
23. Rağbetli MÇ, Aydinlioğlu A, Koyun N, Rağbetli C and Karayel M., 2009. Effect of prenatal exposure to mobile phone on pyramidal cell numbers in the mouse hippocampus: A stereological study. International Journal of Neuroscience, 119, 7, 1031-1041.
24. Rağbetli M.Ç., Aydinlioglu A., Koyun N., Rağbetli C., Bektas Ş., Ozdemir S.,2010 The effect of mobile phone on the numberof Purkinje cells: A stereological study, International Journal of Radiation Biology, vol.86, pp.548-554.
25. Röösli M, Lörtscher M, Egger M, Pfluger D, Schreier N, Lörtscher E, Locher P, Spoerri A, Minder C., 2007. Mortality from neurodegenerative disease and exposure to extremely low-frequency magnetic fields: 31 years of observations on Swiss railway employees. Neuroepidemiology, 28 (4), pp. 197-206.
26. Sahin D, Ozgur E, Guler G, Tomruk A, Unlu I, Sepici-Dinçel A, Seyhan N., 2016. The 2100 MHz radiofrequency radiation of a 3G-mobile phone and the DNA oxidative damage in brain. Journal of Chemical Neuroanatomy 75, 94–98.
27. Sambucci M, Laudisi F, Nasta F, Pinto R, Lodato R, Altavista P, Lovisolo GA, Marino C and Pioli C., 2010. Prenatal exposure to non-ionizing radiation: effects of WiFi signals on pregnancy outcome, peripheral B-Cell compartment and antibody production. Radiation Research, 732-740.
28. Sangun O, Dundar B, Darici H, Comlekci S, Kumbul Doguc D and Celik S., 2015. The effects of long-term exposure to a 2450 Mhz electromagnetic field on growth and pubertal development in female wistar rats. Electromagn Biol Med, 34, 1, 63–71.
29. Schirmacher A, Winters S, Fischer S, Goeke J, Galla HJ, Kullnick U, Ringelstein EB and Stögbauer F., 2000. Electromagnetic fields (1.8 GHz) increase the permeability to sucrose of the blood-brain barrier in vitro. Bioelectromagnetics, 21, 5, 338-345.
30. Schmitz C, Rhodes ME, Bludau M, Kaplan S, Ong P, Ueffing I, Vehoff J, Korr H and Frye CA., 2002. Depression: Reduced number of granule cells in the hippocampus of female, but not male, Rats due to prenatal restraint stres. Molecular Psychiatry, 7, 7, 810-3.
31. Sharma A, Sharma S, Shrivastavaa S, Singhalb PK, Shuklaa S., 2019. Mobile phone induced cognitive and neurochemical consequences. Journal of Chemical Neuroanatomy 102, 1–10.
32. Sırav B., Seyhan, N., 2016. Effects of GSM modulation radio-frequency radiation on permeability of blood-brain barrier in male and female rats. Journal of Chemical Neuroanatomy, 75, 123-127.
33. Takahashi S, Imai N, Nabae K, Kanako K, Kawai H, Wang J, Watanabe S, Kawabe M, Fujiwara O, Ogawa K, Tamano S and Shirai T., 2010. Lack of Adverse Effects of Whole-Body Exposure to a Mobile Telecommunication Electromagnetic Field on the Rat Fetus. Radiation Research, 173, 3, 362-372.
34. Tök L, Nazıroğlu M, Doğan S, Kahya MC and Tök Ö., 2014. Effects of melatonin on Wi-Fi-induced oxidative stress in lens of rats. Indian Journal of Ophthalmology, 62, 12-15.
35. West MJ, Gundersen HJG., 1990. Unbiased stereological estimation of the number of neurons in the human hippocampus. J.Comp.Neurol., 296: 1–22.
36. Wilen J, Johansson A, Kalezic N, Lyskov E and Sandstrom M., 2006. Psychophysiological tests and provocation of subjects with mobile phone related symptoms. Bioelectromagnetics, 27, 3, 204-214.